ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (3): 402-406.doi: 10.11843/j.issn.0366-6964.2015.03.008

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Preliminary Study Parthenogenetic Activation of Phospholipase C zeta (PLCζ) Gene of Sheep Oocytes

MU ERZATI•A Letengbieke,LIU Qiang,HUANG Zeng-wen,MI LAI•Xia Rifu,SAI Wujiafu*   

  1. (College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)
  • Received:2014-10-28 Online:2015-03-23 Published:2015-03-23

Abstract:

Sheep phospholipase C zeta (PLCζ) was used to activate sheep oocytes,to explore whether PLCζ can be used as a new kind of oocyte activator.A pEGFP-N1-PLCζ recombinant plasmid were constructed and then injected into MⅡ sheep oocytes.The effect of PLCζ on activation of oocytes and embryo development were observed and counted.The results showed that pEGFP-N1-PLCζ recombinant plasmid could express PLCζ in oocytes,and could spontaneously activate parthenogenetic development of sheep oocytes.

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