乌骨鸡J亚群白血病的综合诊断及病毒分子生物学特性研究

doi:10.11843/j.issn.0366-6964.2014.03.016

Abstract

Abstract:

The Silkie is a typical Chinese breed of chicken.In recent years，numbers of Silkies eliminated from farms in Liaocheng，were found to be infected with tumor diseases，showing hepatomegaly and splenomegaly.On Dec.2012，6 dead Silkies from a parent farm were found hepatomegaly and splenomegaly with tumor-like nodules by necropsy.By histological detection，PCR testing，virus isolation and identification，ultimately the Silkies were diagnosed myelocytoma and hemangioma，infected by avian leukemia virus subgroup J (ALV-J).To obtain virus genome sequences，four pairs of primers were designed.And finally 7 647 bp sequence，include gag gene (2 016 bp)，pol gene (2 622 bp) and env gene (1 689 bp) were obtained.There was a 205 bp deletion cross rTM (redundant TM) and DR (direct repeat) area，equivalent to 7 049-7 253 position of the prototype ALV-J strain HPRS-103.In the leader sequence of 5′UTR (untranslated region)，there was a 19 bp insertion sequence.The isolate was quite different with the British prototype strain HPRS-103，but was highly similar with stains isolated from commercial layers in recent years.Therefore，this isolate may come from commercial layer，which will bring great challenge to the control.

CLC Number:

S852.659.3

QIN Si-hai，LIU Li-tao，YUE Rui-chao，LI Ning，QU Ya-jin,WANG Guang-wen，HU Dong-fang，LIU Si-dang. Study on Comprehensive Diagnosis and Molecular Characterization of Subgroup J Avian Leukosis Virus from Silkies[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 458-462.

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Study on Comprehensive Diagnosis and Molecular Characterization of Subgroup J Avian Leukosis Virus from Silkies

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