中国美利奴羊皮肤POU2F3基因及其剪接体的克隆与表达分析

doi:10.11843/j.issn.0366-6964.2013.08.003

Abstract

Abstract:

The objective of this study was to explore the expression pattern of POU2F3 gene in sheep, and to clone the full-length CDS of POU2F3 and its alternatively spliced isoforms in Chinese Merino skin (Xinjiang Junken Type). The expression of POU2F3 gene was detected by Semi-quantitative reverse transcription PCR (SqRT-PCR) in tissues. The full-length CDS regions of POU2F3 gene and its isoforms were amplified by RT-PCR, cloned into T vector, and sequenced. The sequence was analyzed using online bioinformatics softwares. SqRT-PCR analysis showed that the POU2F3 gene was only expressed in some sheep internal organs and tissues. Comparatively, it was highly expressed in body side skin. No significant differences at the expression levels of POU2F3 gene between the skins from various anatomic locations of super fine wool strain were observed (P>0.05). Similarly, no significant differences at the expression levels of POU2F3 gene in body side skin between wool and hair sheep were observed (P>0.05). Sequence analysis indicated that sheep POU2F3 gene in skin generated at least four spliced mRNA isoforms encoding four POU2F3 protein isoforms of 429, 396, 221 and 221aa, respectively. POU2F3 gene is highly expressed in the skin of Chinese Merino (Xinjiang Junken Type). Besides the full-length POU2F3, sheep POU2F3 has at least two protein isoforms in skin. This study not only lays the foundation for clarifying the function of sheep POU2F3 gene, but also provides theoretical basis for breeding high quality Merino sheep.

CLC Number:

S826
S813.3

RONG En-guang, QIAO Shu-pei, YU Lei, YAN Xiao-hong,YANG Hua, LI Hui, WANG Ning. Cloning and Expression Characterization of Sheep POU2F3 Gene and Its Isoforms in Chinese Merino Skin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1189-1197.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/ 10.11843/j.issn.0366-6964.2013.08.003

https://www.xmsyxb.com/EN/Y2013/V44/I8/1189

References

［1］ANDERSEN B, SCHONEMANN M D, FLYNN S E, et al. Skn-1a and Skn-1i: two functionally distinct Oct-2-related factors expressed in epidermis［J］. Science, 1993, 260(5104): 78-82.

［2］GOLDSBOROUGH A S, HEALY L E, COPELAND N G, et al. Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11［J］. Nucleic Acids Res, 1993, 21(1): 127-134.

［3］YUKAWA K, YASUI T, YAMAMOTO A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells［J］. Gene, 1993, 133(2): 163-169.

［4］ANDERSEN B, ROSENFELD M G. POU domain factors in the neuroendocrine system: lessons from developmental biology provide insights into human disease［J］. Endocr Rev, 2001, 22(1): 2-35.

［5］JIMENEZ-MATEO O, RODRIGUEZ-TORRES A, AVILA S, et al. The transcription factor PLA-1/SKN-1A is expressed in human placenta and regulates the placental lactogen-3 gene expression［J］. Placenta, 2006, 27(4-5): 357-366.

［6］VEENSTRA G J, VAN DER VLIET P C, DESTREE O H. POU domain transcription factors in embryonic development［J］. Mol Biol Rep, 1997, 24(3): 139-155.

［7］LENA A M, CIPOLLONE R, AMELIO I, et al. Skn-1a/Oct-11 and DeltaNp63alpha exert antagonizing effects on human keratin expression［J］. Biochem Biophys Res Commun, 2010, 401(4): 568-573.

［8］BECK I M, MULLER M, MENTLEIN R, et al. Matrix metalloproteinase-19 expression in keratinocytes is repressed by transcription factors Tst-1 and Skn-1a: implications for keratinocyte differentiation［J］. J Invest Dermatol, 2007, 127(5): 1107-1114.

［9］JAEGLE M, MANDEMAKERS W, BROOS L, et al. The POU factor Oct-6 and Schwann cell differentiation［J］. Science, 1996, 273(5274): 507-510.

［10］PHILLIPS K, LUISI B. The virtuoso of versatility: POU proteins that flex to fit［J］. J Mol Biol, 2000, 302(5): 1023-1039.

［11］RYAN A K, ROSENFELD M G. POU domain family values: flexibility, partnerships, and developmental codes［J］. Genes Dev, 1997, 11(10): 1207-1225.

［12］WEGNER M, DROLET D W, ROSENFELD M G. POU-domain proteins: structure and function of developmental regulators［J］. Curr Opin Cell Biol, 1993, 5(3): 488-498.

［13］HILDESHEIM J, KUHN U, YEE C L, et al. The hSkn-1a POU transcription factor enhances epidermal stratification by promoting keratinocyte proliferation［J］. J Cell Sci, 2001, 114(Pt 10): 1913-1923.

［14］ENOMOTO Y, ENOMOTO K, KITAMURA T, et al. Keratinocyte-specific POU transcription factor hSkn-1a represses the growth of cervical cancer cell lines［J］. Oncogene, 2004, 23(29): 5014-5022.

［15］WELTER J F, GALI H, CRISH J F, et al. Regulation of human involucrin promoter activity by POU domain proteins［J］. J Biol Chem, 1996, 271(25): 14727-14733.

［16］JANG S I, KARAMAN-JURUKOVSKA N, MORASSO M I, et al. Complex interactions between epidermal POU domain and activator protein 1 transcription factors regulate the expression of the profilaggrin gene in normal human epidermal keratinocytes［J］. J Biol Chem, 2000, 275(20): 15295-15304.

［17］SUGIHARA T M, KUDRYAVTSEVA E I, KUMAR V, et al. The POU domain factor Skin-1a represses the keratin 14 promoter independent of DNA binding. A possible role for interactions between Skn-1a and CREB-binding protein/p300［J］. J Biol Chem, 2001, 276(35): 33036-33044.

［18］FISCHER D F, GIBBS S, VAN DE PUTTE P, et al. Interdependent transcription control elements regulate the expression of the SPRR2A gene during keratinocyte terminal differentiation［J］. Mol Cell Biol, 1996, 16(10): 5365-5374.

［19］HILDESHEIM J, FOSTER R A, CHAMBERLIN M E, et al. Characterization of the regulatory domains of the human skn-1a/Epoc-1/Oct-11 POU transcription factor［J］. J Biol Chem, 1999, 274(37): 26399-26406.

［20］HERR W, CLEARY M A. The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain［J］. Genes Dev, 1995, 9(14): 1679-1693.

［21］KATOH Y, KATOH M. Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: Transcriptional mechanisms of FZD5 in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer［J］. Int J Oncol, 2007, 30(3): 751-755.

［22］苏蕊. 内蒙古绒山羊绒毛生长、脱落期皮肤差异表达基因的研究［D］. 呼和浩特: 内蒙古农业大学, 2009.

［23］BLENCOWE B J. Alternative splicing: new insights from global analyses［J］. Cell, 2006, 126(1): 37-47.

［24］CABRAL A, FISCHER D F, VERMEIJ W P, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation［J］. J Biol Chem, 2003, 278(20): 17792-17799.

［25］王宁, 荣恩光, 闫晓红. 毛囊发育与毛发生产［J］. 东北农业大学学报, 2012, 43(9): 6-12.

［26］NIETO L, JOSEPH G, STELLA A, et al. Differential effects of phosphorylation on DNA binding properties of N Oct-3 are dictated by protein/DNA complex structures［J］. J Mol Biol, 2007, 370(4): 687-700.

［27］ZHANG Z, LIAO B, XU M, et al. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1［J］. FASEB J, 2007, 21(12): 3042-3051.

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[3]	SONG Pengyan, WANG Siwei, YUE Qiaoxian, ZHANG Yinliang, CHEN Xiaoyong, ZHOU Rongyan. Identification of oar-miR-200b Promoter and Effects on Mitochondrial Function in Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5066-5076.
[4]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[5]	CHEN Conghui, DUAN Chunhui, YANG Xinyu, XIA Cui, GUO Yunxia, JI Shoukun, YAN Hui, LIU Yueqin, ZHANG Yingjie. Effects of Different Treatments of Transportation Stress on Energy Metabolism of Lambs Muscle after Slaughter [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5101-5111.
[6]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[7]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[8]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[9]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[10]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[11]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[12]	LI Yuexin, LIU Aiju, MA Xiaofei, ZHENG Zhong, HU Boxin, ZHI Yunxia, TIAN Shujun. Effect of TGFβR1 on the Function of Sheep Granulosa Cells Mediated by TGF-β/Smad Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3335-3347.
[13]	MA Youji, CHEN Pengfei, MA Qing, WU Yi. Effects of Different Exercise Amounts on Rumen Microflora Diversity of Tan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3393-3405.
[14]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[15]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.

Cloning and Expression Characterization of Sheep POU2F3 Gene and Its Isoforms in Chinese Merino Skin

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments