LH/hCG受体与cAMP在玉米赤霉烯酮抑制小鼠Leydig细胞分泌睾酮中作用的研究

doi:10.11843/j.issn.0366-6964.2013.07.021

Abstract

Abstract:

To reveal the reproductive toxicity mechanism of zearalenone(ZEN) to male animals, the role of LH/hCG receptor and cAMP in zearalenone inhibiting testosterone secretion in mouse Leydig cells has been researched. Primary Leydig cells in vivo were taken to determine the testosterone level (hCG or cAMP stimulated) and cAMP level (hCG stimulated) after exposed to 0, 1, 5, 10, 20 μg·mL^－1 zearalenone for 12 h or to 2.5 μg·mL^－1 for 1, 6, 12, 24 h by ELISA, determined binding activity of LH/hCG receptor by Chemiluminescence Immunoassay (CLIA), and determined LH/hCG protein expression level by Western Blot. The results showed that in hCG environment long time and high dose zearalenone exposure inhibited testosterone secretion extremely significant (P<0.01), in cAMP environment every dosage and time exposure for zearalenone reduced testosterone secretion significant (P<0.05), and the time- or concentration- effect relationship was obvious. Every exposure condition effected neither LH/hCG receptor binding activity nor protein expression obviously (P>0.05), and low concentration and long time zearalenone exposure could increase the intercellular cAMP level significantly (P<0.05). These results indicate that zearalenone can inhibit testosterone secretion through cAMP pathway but not LH/hCG pathway.

CLC Number:

LIU Qing, WANG Ya-jun, XU Hui, HUANG Qin-yi, GU Jian-hong, YUAN Yan,LIU Xue-zhong, LIU Zong-ping, BIAN Jian-chun. The Role of LH/hCG Receptor and cAMP in Zearalenone Inhibiting Testosterone Secretion in Mouse Leydig Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1147-1154.

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The Role of LH/hCG Receptor and cAMP in Zearalenone Inhibiting Testosterone Secretion in Mouse Leydig Cells

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