崂山奶山羊硬脂酰-CoA去饱和酶基因（SCD）多态性及其与泌乳性状的相关分析

doi:10.11843/j.issn.0366-6964.2013.07.019

Abstract

Abstract:

The current study was aimed to analyze the association of exon 3 of stearoyl-CoAdesaturase gene (stearoyl-CoA desaturase, SCD) with milk traits in Laoshan dairy goat (Capra hircus). Direct sequencing was applied to identify SNPs in exon 3 and its flanking regions of SCD gene in Laoshan dairy goat population containing 174 samples, and least square method was performed to evaluate the association of polymorphism in SCD with milk traits. Four SNPs have been identified as followed, E315 E/e and E368H/h in exon3, I346 R/r and I355Q/q in in eron3 of SCD gene. The distribution of alleles and genotypes in this group were all under Hardy-Weinberg equilibrium. And the alleles and genotypes distribution of E315 were consistent with that of I355, so the two sites were linked sites. The difference between genotypes EE/QQ and ee/qq was statistically significant for all milk ingredients (P<0.05 or P<0.01).And the difference between genotypes EE/QQ and Ee/Qq was significant for protein rate, milk density (P<0.05). Allele E/Q had positive effects on all milk ingredients, in which the additive effects were highly significant (P<0.01) and the dominant effects were not significant (P>0.05). The difference between genotype hh with genotypes HH and Hh was statistically significant for all milk ingredients (P<0.05 or P<0.01). Meanwhile the allele h had positive effects on all milk ingredients. The additive effects and the dominant effects of allele on all milk ingredients were both highly significant (P<0.01). The milk yield of genotype rr was significantly higher than that of RR (P<0.01). Allele r had positive effect on milk yield, while it had no significant effect on milk ingredients (P>0.05). Then we analyzed the association of polymorphisms in SCD gene with milk traits in Group F and Group P respectively. The difference among genotypes were very low on milk traits in Group F, while that was higher in Group P. Four SNPs have been identified in exon 3 and its flanking regions of SCD gene, E315 E/e, E368H/h I355Q/q and I346 R/r, in which I346 R/r had not yet been found in other domestic dairy goats. Alleles E/h/Q had positive effects on all milk ingredients and allele r had positive effect on milk yield. The effects of SCD gene on milk traits of Laoshan dairy goats mainly be owing to the influence of Group P.

CLC Number:

WANG Gui-zhi,WANG Jin-feng, QIN Zi-juan, WANG Jian-min,ZHAO Jin-shan, CHENG Ming, WANG Jian-hua. Association between Polymorphism of Stearoyl-CoA Desaturase Gene (SCD) and Milk Production Traits in Laoshan Dairy Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1131-1139.

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Association between Polymorphism of Stearoyl-CoA Desaturase Gene (SCD) and Milk Production Traits in Laoshan Dairy Goat

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