1株髓细胞瘤型J亚群禽白血病病毒感染性克隆的构建与病毒拯救

doi:10.11843/j.issn.0366-6964.2013.05.012

Abstract

Abstract:

The objective of this study was to construct the infectious molecular clone with molecular marker of subgroup J avian myeloid leukosis virus (ALV-J) strain SCGS-1. A full-length infectious clone of ALV-J (pUC-SCGS) was constructed by cloning and combining of three fragments using PCR method from SCGS-1. SalⅠ site was introduced on 4684nt of SCGS-1 by overlapping PCR to form another infectious clone and named pUC-△SCGS. The two plasmids, pUC-SCGS and pUC-△SCGS, were transfected into CEF, and the rescued viruses were detected by PCR, avian leukosis virus antigen test kit and indirect immunofluorescence assay (IFA). Digestion and sequence analysis revealed that the infectious clone pUC-SCGS and pUC-△SCGS were constructed correctly. PCR, ELISA test and IFA results showed that the 3rd and 4th generation of rescued virus were positive, while the controlled CEF were negative. Rescued virus and the virus with molecular marker of subgroup J avian myeloid leukosis virus were successfully constructed, named rSCGS-1 and r△SCGS-1.

CLC Number:

S852.659.3

LIN Yan, XIA Jing, ZOU Nian-li, GUO Ming-ping, WANG Fu-yan, ZHAO Yang, WEN Xin-tian, CAO San-jie, HUANG Yong. Construction and Virus Rescue of Infectious Molecular Clones of Subgroup J Avian Myeloid Leukosis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 754-759.

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Construction and Virus Rescue of Infectious Molecular Clones of Subgroup J Avian Myeloid Leukosis Virus

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