山羊肌肉组织microRNA Solexa测序与生物信息学分析

Abstract

Abstract:

Characterization of microRNA expression in goat muscle was studied, with the purposes of providing basic information for further studies of goat growth and development activities such as goat muscle cell proliferation and differentiation. The small RNAs isolated from total RNA of goat muscle were sequenced by Solexa and then bioinformatics analysis was perfomed. Meanwhile, the expression of selected miRNA was validated by q-PCR. Based on Solexa sequencing and bioinformatics analysis, 517 muscle miRNAs, which are conservative on evolution of mammals (sheep, bovine, swine, horse, dog), and 2 goat genome-specific miRNA were identified. Among them, 306 exceeded 100 in the expression levels. The expression of 8 selected miRNA in muscle tissues obtained by q-PCR was agreement with Solexa sequencing results. MiRNA target gene prediction and functional analysis showed that many miRNAs among them may affect cell signaling pathways and animal muscle development. Succeed in construction of expression profiling of miRNA which are abundant and differentially expressed in goat muscle tissues. This study provides important information on the role of miRNA regulation in muscle growth and development.

CLC Number:

S827.2

LING Ying-hui, ZHANG Xiao-dong,WANG Li-juan1, YIN Jie, XIANG Hao, LI Yun-sheng, ZHANG Yun-hai,ZHANG Zi-jun, DING Jian-ping, ZHANG Xiao-rong. Solexa Sequencing and Bioinformatics Analysis on MicroRNA from the Goat Muscle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Solexa Sequencing and Bioinformatics Analysis on MicroRNA from the Goat Muscle

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