Abstract:

This experiment was conducted to compare the pathogenicity of ALV-J related acute fibrosarcoma extract inoculated on embryos and chicks. Acute fibrosarcoma extract was inoculated on yolk sac of 5-day-old-embryos, chorioallantoic membrane of 11-day-old-embryos and intraperitoneal inoculation of 1-day-old SPF chicks to compare pathogenicity of different inoculation methods. The results showed that the mortality of yolk sac inoculation was 14/30 at the age of 18-22 days, the incidence of visceral sarcoma was 8/14. The mortality of Chorioallantoic membrane inoculation was 17/30 at the age of 18-22 days, the incidence of visceral sarcoma was 6/17. As to the pathogenicity of the chicks, 13 hatched chicks from the chorioallantoic membrane inoculation group were all died, 11 of them developed visceral sarcoma. These results indicated that the pathogenicity of chorioallantoic membrane inoculation is higher than those inoculated on yolk sac of 5-day-old-embryos and those intraperitoneal inoculation of 1-day-old SPF chicks. Chorioallantoic membrane inoculation not only caused high incidence of visceral sarcoma, but also the sarcoma occurs earlier and faster, so it can be used as further acute fibrosarcoma model of artificial disease.