Abstract:

This study aimed to investigate the expression abundance of miR-1 and miR-133 in the highest longissimus dorsi muscle from 180-day-old pigs including Landrace, Lantang and Dahuabai pigs using Real-time quantification of microRNAs by stem-loop RT-PCR method. The results showed that miR-1 abundance was the highest in Lantang, and 4.7 times higher than that in Landrace and 6.4 times higher than that in Dahuabai pigs(P<0.05), respectively, but not significantly different(P>0.05) between Landrace and Dahuabai pigs. miR-133 abundance was also the highest in Lantang, followed by Landrace and Dahuabai pigs, the former two were significantly 7.2 and 5.7 times higher(P<0.05) than that in the latter, respectively, but there was no significant differences(P>0.05) between Landrace and Lantang pigs. The results indicate that miR-1 and miR-133 expression are specific among the pig breeds, suggesting that the abundance of miR-1 and miR-133 are associated with meat quality traits in pigs.