Table 1

the sequences of the primers"

Fig. 1

Identification of PCR products of variable regions of heavy and light chains of mouse-derived McAb G12 by electrophoresis A. Identification of PCR product of G12 VH (M. DL5000 DNA marker; 1-2. PCR product of VH; C. Control); B. Identification of PCR product of G12 VL (M. DL5000 DNA marker; 1-4. PCR product of VL; C. Control)"

Fig. 2

Identification of the expression of scFv by induction and its purified products A, B. Identification of the expression of scFv by SDS-PAGE, Western blot [M. Molecular weight standard for prestained proteins; 1. pET28a/BL21 (DE3) empty vector control; 2. pET28a-scFv/BL21 (DE3) induced by IPTG; 3. The supernatant of pET28a-scFv/BL21 (DE3) after ultrasonication and centrifugation; 4. The precipitate of pET28a-scFv/BL21 (DE3) after ultrasonication and centrifugation]; C. SDS-PAGE identification of purified scFv by urea gradient dialysis method [M. molecular weight standard for prestained proteins; 1. pET-28a/BL21 (DE3) empty vector control; 2. Purified scFv protein (0.433 5 mg·mL-1); 3. Purified scFv protein (0.313 5 mg·mL-1)]"

Fig. 3

Identification of the expression of scFv-Fc by induction and its purified products A, B. Identification of the expression of scFv- Fc by SDS-PAGE, Western blot [M. Molecular weight standard for prestained proteins; 1. pET28a/TSsetta (DE3) empty vector control; 2. pET28a-scFv-Fc/TSsetta (DE3) by IPTG]; C. Identification of the expression form of scFv-Fc by SDS-PAGE [M. Molecular weight standard for prestained proteins; 1. pET28a/TSsetta (DE3) empty vector control; 2. pET28a-scFv-Fc/TSsetta (DE3) induced by IPTG; 3. Supernatant of pET28a-scFv-Fc/TSsetta (DE3) after ultrasonication and centrifugation; 4. Precipitate of pET28a-scFv-Fc/TSsetta (DE3) after ultrasonication and centrifugation]; D. SDS-PAGE identification of purified scFv-Fc by urea gradient dialysis method [M. Molecular weight standard for prestained proteins; 1, 2. Purified scFv-Fc protein (0.2 mg ·mL-1)]"

Fig. 4

Detection of antibody titers of purified scFv and scFv-Fc by ELISA"

Fig. 5

Identification of the specificity of scFv and scFv-Fc by IPMA A. Identification of the reactivity of scFv, scFv-Fc with CDV H protein by IPMA(bar=100 μm); B. Identification of the reactivity of scFv, scFv-Fc with CDV by IPMA(bar=50 μm)"

Fig. 6

Effects of scFv and scFv-Fc on H/F-mediated membrane fusion(bar=100 μm) A. CDV H/F-mediated membrane fusion depends on SLAM receptor; B. The inhibitory effects of the purified G12、scFv-Fc、scFv and 2D9 antibodies on H/F/SLAM-mediated membrane fusion"