绵羊痒螨重组丝氨酸蛋白酶抑制剂对小鼠银屑病样皮肤炎症的影响

doi:10.11843/j.issn.0366-6964.2024.12.039

Abstract

Abstract:

Our team previously found that recombinant serine protease inhibitors 4 and 5 (rPsoSP4 and rPsoSP5) of Psoroptes ovis could inhibit the enzymatic activities of chymotrypsin, trypsin and elastase in vitro, thus we speculated that these two recombinant proteins might have anti-inflammation activities. To confirm the above speculation, we assessed whether rPsoSP4 and rPsoSP5 could improve psoriatic-like skin inflammation in mice. BALB/c mice were randomly divided into 5 groups (n=6), Vaseline or imiquimod cream (IMQ) was applied daily on the back skin continuously for 6 days to establish the control group and psoriasis model groups. During this period, the psoriasis model groups were intradermal injected with PBS (IMQ group) or pET32a(+)-vector protein (IMQ+pET32a(+) group) or rPsoSP4 (IMQ+rPsoSP4 group) or rPsoSP5(IMQ+rPsoSP5 group), then the changes of psoriasis-like skin lesions were observed every day, and mice were sacrificed on the 7^th day. Subsequently, the pathological changes and the transcription levels of IL-1β, IL-6, and TNF-α in the skin lesions among each group were compared. Results showed that IMQ induced psoriasis-like lesions were successfully established, and the mice appeared typical psoriasis-like lesions. Compared with the model group (IMQ group), IMQ+rPsoSP4 group can effectively attenuated psoriasis-like skin lesions, including significant inhibition of weight loss (from 4^th to 6^th day, P < 0.05, P < 0.01, P < 0.001) and reductions of skin erythema (5^th and 6^th day), skin scales (from 4^th to 6^th day), skin thickness (from 4^th to 6^th day) and PASI score (from 3^rd to 6^th day) in mice (P < 0.05, P < 0.001). Additionally, compared with the model group (IMQ group), IMQ+rPsoSP4 group showed that the pathological damage in the skin lesion was significantly reduced, accompanied by the significant decrease in epidermal thickness (P < 0.05) and the number of infiltrated inflammatory cells (P < 0.001). Moreover, there was a significantly decreased in mRNA levels of IL-1β, IL-6, and TNF-α in the skin lesion of IMQ+rPsoSP4 group in comparison with IMQ groups, while IMQ+rPsoSP5 group had no significant improvement on psoriatic lesions. In conclusion, rPsoSP4 could significantly improve psoriasis skin lesion in mice and has significant anti-inflammatory activity, while rPsoSP5 seemed to be no obvious effect.

Key words: Psoroptes ovis, rPsoSP4, rPsoSP5, psoriasis-like skin inflammation, anti-inflammatory activity

CLC Number:

S857.5

TIAN Yan, YANG Fusheng, LI Yan'e, LIANG Youping, FAN Jie, WU Fangyan, GU Xiaobin. Effect of Recombinant Serpins of Psoroptes ovis on Psoriasis-like Skin Inflammation in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5774-5783.

Figures/Tables 4

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References 34

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Effect of Recombinant Serpins of Psoroptes ovis on Psoriasis-like Skin Inflammation in Mice

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