广灵驴分子系谱的建立及群体遗传结构分析

doi:10.11843/j.issn.0366-6964.2022.02.007

Abstract

Abstract: This study aimed to construct the molecular pedigree based on investigating of Guangling donkey population in breeding farm, and analyze its genetic structure. A total of 107 (including 13 males and 94 females) jugular vein blood of healthy adult donkey (body weight:350-400 kg) were collected, after condensant treatment, the DNA was extracted. After using 12 SSR markers for fluorescence PCR amplification, the products were genotyped on ABI3730 sequencing instrument. The molecular pedigree was constructed by Cervus 2.0 and Pedigraph 2.4 softwares, and the population genetic parameters were calculated by STRUCTURE2.3 and Fstat softwares. Moreover, the phylogenetic tree(NJ tree)of 7 male donkey and their offspring was established by hclust function of R software. In all, 107 donkey were included in the molecular pedigree and only 30 offspring were matched with their fathers, and 7 offspring were matched with their mothers, the reliance level of pedigree was higher than 90%. The H_Ob and PIC of SSR markers were 0.676 5 and 0.593 9, respectively. NJ phylogenetic tree included 7 male families, the F_ST of the current population was 0.184, the F_IT was 0.033, and F_IS was -0.238, the population was in Hardy-Weinberg equilibrium. This study constructed a high reliable molecular pedigree of Guangling donkey and analyzed its genetic structure. The results show that the Guangling donkey population has high genetic polymorphisms, with low inbreeding coefficient. Therefore, it is worthful to preserve the genetic information and develop its breed potentials.

Key words: Guangling donkey, microsatellite markers, molecular pedigree, population genetic structure, polymorphism

CLC Number:

S822.2

NIU Xiaoyan, CAO Liang, MING Shiqing, LI Yanping, ZHAN Haijie, JIANG Zhiguang, WEI Shunsheng, ZHANG Yuanqing. Construction of Molecular Pedigree and Analysis of Population Genetic Structure of Guangling Donkey[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 391-401.

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Construction of Molecular Pedigree and Analysis of Population Genetic Structure of Guangling Donkey

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