精密组织切片的制备及其应用

doi:10.11843/j.issn.0366-6964.2022.02.002

Abstract

Abstract: Precision-cut tissue slices are widely used by many researchers as in vitro models of organs, preserving the major advantages of in vitro systems, compensating for the lack of functionality of cell lines, and maintaining tissue activity, functional activity sufficiently for toxicity, induction and infection studies. This paper reviews the general development process, advantages and application prospects of precision tissue sectioning techniques, and reviews the preparation methods, culture systems and activity determination indexes of precision-cut tissue slices,as well as the application of precision-cut tissue slices as a novel ex vivo model for the study of various organs. It is expected to provide reference for subsequent researchers to use precision-cut tissue slices as a novel biological in vitro, ex vivo model for research.

Key words: precision-cut tissue slices, ex vivo models, drug metabolism, viral infection, pathogenesis

CLC Number:

S852.16

ZHANG Yiling, KAN Zifei, NIU Zheng, YU Qiuhan, RAN Ling, ZHANG Shujuan, ZOU Hong, XU Shasha, ZHANG Jingyi, SONG Zhenhui. Preparation and Application of Precision-Cut Tissue Slices[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 339-348.

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Preparation and Application of Precision-Cut Tissue Slices

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