北京黑猪FUBP3和USP43基因多态性与眼肌面积性状的关联分析

doi:10.11843/j.issn.0366-6964.2022.12.007

Abstract

Abstract: The study aimed to investigate the relationship between far upstream element binding protein 3 (FUBP3) or ubiquitin specific peptidase 43 (USP43) gene polymorphisms and loin muscle area traits in Beijing black pigs, so as to guide the breeding work at the molecular level. A total of 408 Beijing black pigs were selected to collect data on loin muscle area traits and meat samples for DNA extraction. A total of 43 pairs of primers were designed for PCR amplification and sequencing according to FUBP3 and USP43 genes sequence, and the sequencing results were analyzed by DNAstar software. Association analysis was performed on different genotypes of FUBP3 and USP43 genes with loin muscle area traits of Beijing black pigs, and gene expression differences of the two genes were analyzed by fluorescent quantitative PCR. There were 8 mutations in the promoter region of FUBP3 gene, among which rs701769847G>A was significantly associated with the 5-6 rib loin muscle area and the last rib loin muscle area(P<0.05), rs332131528C>G was only significantly associated with 5-6 rib loin muscle area (P<0.05), rs325550799T>C, rs339464012T>C and rs326069041T>C were only significantly associated with the last rib loin muscle area (P<0.05). Differential gene expression analysis showed that the mRNA level of FUBP3 gene in individuals with GG genotype at rs701769847G>A was significantly lower than those with AA genotype(P<0.05). rs335310752C>T in the promoter region and rs323463345G>A in the shear region of USP43 gene were both significantly associated with the last rib loin muscle area (P<0.05). Differential gene expression analysis showed that the mRNA levels of USP43 gene in rs323463345G>A were not significantly different between GG-type individuals and AA-type individuals. Two loci in the above two genes were significantly associated with 5-6 rib loin muscle area trait (P<0.05), and 6 loci were significantly associated with the last rib loin muscle area traits(P<0.05)， which can be used as candidate genes for loin muscle area trait variation in Beijing black pigs, and as potential molecular markers for loin muscle area traits.

Key words: Beijing black pigs, FUBP3, USP43, loin muscle area

CLC Number:

S828.2

HOU Renda, ZHANG Run, NIU Naiqi, YANG Man, HUANG Xiaoyu, LI Huihui, ZHANG Longchao. Association of FUBP3 and USP43 Gene Polymorphisms with Loin Muscle Area in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4197-4206.

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Association of FUBP3 and USP43 Gene Polymorphisms with Loin Muscle Area in Beijing Black Pigs

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