天祝白牦牛NGB基因的克隆及生物信息学分析

Abstract

Abstract:

In order to elucidate the structures of NGB gene, the nearly complete NGB gene sequence of Tianzhu White yak was amplified and analyzed through PCR, cloning, sequencing and the bioinformatics tools. The results showed that the sequence of NGB gene was 3 840 bp in length, including four exons and three introns. 151 amino acids were encoded by the NGB gene and the cloning existed codon bias phenomenon. Compared with NGB gene and its amino acid sequences in taurine cattle (Bos taurus), there were two nucleotide deletions in intron 3 of NGB gene of Tianzhu White yak and two changes of amino acids occurred at the 83^rd (S→P) and the 97^th (R→Q) positions, respectively. The results lay a foundation for further studying on the genetic characteristics and physiological mechanisms of NGB.

CLC Number:

S823.85

LI Sheng-jie，DU Xiao-hua，LUO Yu-zhu，LIU Xia. Cloning and Bioinformatics Analysis of NGB Gene of Tianzhu White Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Cloning and Bioinformatics Analysis of NGB Gene of Tianzhu White Yak

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