在慢性热应激环境下外源性褪黑素干预对肉鸡皮质酮合成的影响

doi:10.11843/j.issn.0366-6964.2025.11.046

Abstract

Abstract:

Chronic heat stress seriously affects the production performance of poultry. The purpose of this study was to investigate the mechanism of exogenous melatonin (Mel) on the synthesis and secretion of corticosterone in broilers under chronic heat stress, and to provide a theoretical basis for melatonin to alleviate chronic heat stress. Ninety 2-week-old broilers were randomly divided into 6 groups: normal control group (CON), chronic heat stress (HS) group, melatonin intervention group: CONH (2.0 mg·kg^-1) group, HSL (0.5 mg·kg^-1) group, HSM (1.0 mg·kg^-1) group, HSH (2.0 mg·kg^-1) group. The experiment lasted for 21 days. Results: Chronic heat stress resulted in a decrease in broiler performance and a significant increase in serum corticosterone content, while the performance of broilers treated with 0.5 mg·kg^-1 Mel was significantly improved (P < 0.001), and the feed-to-weight ratio of broilers in the HSH group was significantly decreased (P < 0.05). Dietary supplementation with 0.5-2.0 mg·kg^-1 Mel significantly reduced serum corticosterone content in broilers under chronic heat stress (P < 0.000 1). In addition, Mel could effectively improve the decrease of reduced glutathione (GSH) content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity caused by chronic heat stress. The network pharmacology analysis showed that Mel intervention in chronic heat stress was enriched in the cAMP signaling pathway. It was detected that the mechanism of Mel was related to the melatonin receptor MT1, and by inhibiting the cAMP/PKA/CREB signaling pathway, the synthesis and secretion of adrenocortical ketone were weakened, and the stress response was alleviated.

Key words: broiler chicken, chronic heat stress, corticosterone, exogenous melatonin, adrenal gland

CLC Number:

S852.2

KANG Xin, XU Xiangyang, HAN Aige, SONG Fei, RUI Huiyuan, JIANG Xiaowen, GE Ming, YU Wenhui. Effect of Exogenous Melatonin Intervention on Corticosterone Synthesis in Broilers under Chronic Heat Stress Conditions[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5912-5924.

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抗体 Antibodys	来源 Source	货号 Identifier	稀释比例 Dilution ratio
Anti-HSP70 antibody	Bioss	bs-0244R	1∶1 000
Anti-MT1 antibody	Bioss	bs-0027R	1∶1 000
Anti-MT2 antibody	Bioss	bs-0963R	1∶1 000
Anti-PKA antibody	Bioss	bs-0520R	1∶1 000
Anti-CYP11A1 antibody	Bioss	bs-3608R	1∶1 000
Anti-CREB antibody	Wanleibio	WL01848	1∶1 000
Anti-CYP11B1 antibody	Wanleibio	WL05422	1∶1 000
Anti-P-CREB antibody	Abways	CY5043	1∶1 000
Anti-StAR antibody	Abclonal	A22166	1∶2 000
Anti-Rabbit IgG H&L antibody	Bioss	bs-0295G-HRP	1∶10 000
Anti-Beta tubulin antibody	Bioss	bs-4511R	1∶10 000

基因名称 Gene names	序列(5′ → 3′) Sequence	方向 Orientation	NCBI编号 NCBI No.
GAPDH	TATCTTCCAGGAGCGTGAC	Forward	NM_204305.2
	ATCTGCCCATTTGATGTTGC	Reverse
MT1	TCCTGGTCATCCTCTCGGT	Forward	NM_205362.2
	CGTTCCGCAGTTTCTTGTTG	Reverse
MT2	TGCTGATCTTCACCACCGT	Forward	NM_001293103.2
	AGGTTGCCCAAAATGTCCAC	Reverse
PKA	ATCACGTCTCTGAACTTGCT	Forward	XM_025152948.2
	ACTCTTCCGAATGATCCTGT	Reverse
CREB	CTAATAAGGCAGTAGATACCG	Forward	XM_040653773.2
	GAACACGTTTAACTTCCACT	Reverse
CYP11A1	TGGCTCAACCTGTACCACT	Forward	NM_001001756.2
	ACGTTGTGGAAGCCTCCCTC	Reverse
HSD3B1	CCCTCAATCATCCCCAGA	Forward Reverse	XM_046906757.1
	TTATTCAACATCTCCGTGCT	Reverse

项目Item	组别Group
项目Item	CON	CONH	HS	HSL	HSM	HSH
0-7 d ADG	57.14±4.59	48.57±4.19	42.38±2.42	44.76±1.67	49.05±2.68	32.62±6.67
7-14 d ADG	51.11±5.96	61.98±2.63	51.98±3.02	55.02±4.68	38.10±1.46	39.60±1.93
14-21 d ADG	78.17±3.61	57.22±2.24^*	38.49±3.95^####	63.17±3.79^@@	53.25±4.40	46.35±2.01
0-21 d ADG	62.14±1.17	55.93±1.20	44.29±1.55^###	54.32±2.91^@	46.80±0.88	39.52±1.85
胸肌指数/% Breast muscle index	0.182±0.001	0.165±0.005	0.161±0.006	0.143±0.002	0.165±0.008	0.192±0.006^@
F/G	2.770±0.028	2.624±0.058	2.783±0.028	2.671±0.028	2.802±0.010	2.587±0.065^@

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Effect of Exogenous Melatonin Intervention on Corticosterone Synthesis in Broilers under Chronic Heat Stress Conditions

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