艾叶精油体外抑制鸡马立克病病毒增殖的效应分析

doi:10.11843/j.issn.0366-6964.2025.11.045

Abstract

Abstract:

This study aims to investigate the effects of Artemisia argyi essential oil on the in vitro replication of Marek's disease virus and elucidate the underlying molecular mechanisms involved. The cytotoxicity of AAEO on chicken embryo fibroblast (CEF) cells was determined by the CCK-8 method, and the optimal concentration range of AAEO was screened; The cells were treated and samples were collected according to the selected optimal concentration. The effect of AAEO on the proliferation of MDV in vitro was detected by plaque analysis and virus titer assay.The mechanism of AAEO inhibiting MDV proliferation was analyzed at the molecular level by RT-qPCR and Western blot. The results showed that AAEO had no cytotoxic effect on CEF cells in the range of 60-360 μg·mL^-1.In the AAEO treatment group, the virus plaque was significantly reduced and reduced, the virus titer was decreased, and the expression of virus at the gene level and protein level were decreased, indicating that AAEO can inhibit the replication of MDV in a dose-dependent manner. Through the screening of intracellular signaling pathways of AAEO, it was found that AAEO could down regulate the expression levels of phosphorylated ERK and STAT3 proteins, and down regulate the expression levels of IL-6 mRNA. Treatment of cells with the ERK inhibitor U0126 reduced viral replication when the intracellular ERK pathway was inhibited. AAEO effectively inhibits the in vitro proliferation of MDV in chickens. The antiviral mechanism may be associated with its suppression of IL-6/STAT3 signaling pathway and ERK pathway activation. This study demonstrates that AAEO exhibits significant anti-MDV activity, and the findings provide novel evidence for developing therapeutic agents against herpesviruses. The research outcomes offer new insights into the exploration of antiviral drug candidates targeting herpesvirus infections through natural product derivatives.

Key words: Marek's disease virus (MDV), Artemisia argyi essential oil (AAEO), Chicken embryo fibroblast cells, in vitro antiviral

CLC Number:

S859.7

ZHANG Yuan, LI Mingyang, LIANG Yue, YU Xiangqian, WANG Yan, CHEN Jiyi, LIU Haixia, LIAN Xue, QIAN Yingjuan. Analysis of the Effect of Artemisia argyi Essential Oil on the Inhibition of the Proliferation of Chicken Marek's Disease Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5901-5911.

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References 29

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抗体 Antibodies	编号 Identifier	来源 Source	宿主 Host	稀释比例 Dilution rate
actin	66009-1-Ig	武汉三鹰生物技术有限公司	鼠	1∶30 000
ERK1/2	11257-1-AP	武汉三鹰生物技术有限公司	兔	1∶1 000
pERK	Cat No. 28733-1-AP	Cell Signaling Technology	兔	1∶500
STAT3	AP0365	bioworld	兔	1∶1 000
p-STAT3(B-7)	#10222	Santa Cruz Biotechnology	鼠	1∶500
VP22	无	以VP22全长为抗原免疫小鼠制备，本实验室保存	鼠	1∶20 000
Goat Anti-Rabbit IgG (H+L) HRP	BS13278	bioworld	兔	1∶10 000
Goat Anti-Mouse IgG (H+L) HRP	BS12478	bioworld	鼠	1∶10 000

引物名称 Primers name	引物对序列(5′→3′) Primer paris suquences
actin	F：GAGACCTTCAACACCCCAGCCATG；R：GCGACGTAGCACAGCTTCTCCTTG
vp22	F：ATCGGATCCATGGGGGATTCTGAAAG；R：ACACTCGAGTTATTCGCTATCACTGC
IL-6	F：CAGGACGAGATGTGCAAG；R：GTAGCACAGAGACTCGACGT

Analysis of the Effect of Artemisia argyi Essential Oil on the Inhibition of the Proliferation of Chicken Marek's Disease Virus in vitro

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