Table 1

Primers sequence information of CYP3A29 promoter fragment amplification"

Table 2

Primers sequence information of RUNX1 gene interference vector"

Fig. 1

Tissue expression profile of CYP3A29 gene A. The mRNA expression levels of CYP3A29 gene in various tissues; B. The expression levels of CYP3A29 protein in various tissues. 1-7 represent heart, liver, spleen, lung, kidney, small intestine, muscle.HPRT, GAPDH are internal reference genes"

Fig. 2

Segmental amplification of CYP3A29 promoter and vector double enzyme digestion verification A. Segmental amplification of the CYP3A29 gene promoter: 1-4.Amplified fragments of CYP32A29 gene promoter from-2 026-+62 bp, -1 526-+62 bp, -1 026-+62 bp and -528- +62 bp; B. Validation of a luciferase reporter vector for the CYP329 gene promoter by double enzymatic cleavage: 1-2. Validation of pRL-TK plasmid with double digestion; 3-4. pGL4.10-CYP3A29(-2 026- +62 bp) plasmid verified with double digestion; 5-6. pGL4.10-CYP3A29(-1 526- +62 bp) plasmid verified with double digestion; 7-8. pGL4.10-CYP3A29(-1 026- +62 bp) plasmid verified with double digestion; 9-10. pGL4.10-CYP3A29(-528- +62 bp) plasmid verified with double digestion"

Fig. 3

Identification of the core promoter of the CYP3A29 gene A. Validation of transfection efficiency of 293T and AML12 cells; B. Expression activity of different length promoters of CYP3A29 gene in 293T cells; C. Expression activity of different length promoters of CYP3A29 gene in AML12 cells"

Fig. 4

Analysis of the segmentation activity of the core promoter of the CYP3A29 gene A. Segmental amplification of the core promoter of CYP3A29 gene: 1-3.Amplified fragments of core promoter of CYP3A29 gene from-448-+62 bp, -289-+62 bp and-96-+62 bp. B. Validation of a luciferase reporter vector for the CYP329 gene promoter by double enzymatic cleavage: 1-2. pGL4.10-CYP3A29(-448-+62 bp) plasmid verified with double digestion; 3-4. pGL4.10-CYP3A29 (-289-+62 bp) plasmid verified with double digestion; 5-6. pGL4.10-CYP3A29(-96-+62 bp) plasmid verified with double digestion. C. Expression activity of different length of core promoter of CYP329 gene in AML12 cells"

Fig. 5

Expression levels of Spi1, Spib and RUNX1 transcription factors A. Expression levels of HPRT and Spi1 genes in AML12 cells; B. Expression levels of RUNX1 and Spib genes in AML12 cells.1-8 represent AML12 cell, heart, liver, spleen, lung, kidney, small intestine, muscle(mouse).HPRT is internal reference gene"

Fig. 6

Effect of the RUNX1 mutant site on the activity of CYP3A29 core promoter A. Binding sites for the RUNX1 transcription factor; B. Mutant sequences of the RUNX1 transcription factor; C. Expression activity of RUNX1 wild-type and mutant promoters in AML12 cells"

Fig. 7

Effect of RUNX1 interference on the core promoter activity of the CYP3A29 gene A. Efficiency assay of RUNX1 interference at the mRNA level; B. Efficiency assay of RUNX1 interference at the protein level; C. Effect of RUNX1 interference on the activity of the RUNX1 wild-type promoter; D. Effect of RUNX1 interference on the activity of the RUNX1 mutant promoter"