基于单细胞测序技术探讨动物骨骼肌卫星细胞与生态位细胞之间的“对话”

doi:10.11843/j.issn.0366-6964.2024.12.002

Abstract

Abstract:

Single-cell sequencing is a cutting-edge technology for high-throughput sequencing of genomes, transcriptomes, and epigenomes of individual cells, offering a powerful tool to characterize individual cells and elucidate interactions between cells, and has provided many new insights in tumorigenesis and stem cell biology. Mammalian skeletal muscle is a heterogeneous tissue dominated by myofibers, including various kinds of cells such as muscle satellite cells, fibro-adipogenic progenitors and macrophages, et al. Benefiting from the rapid development of single-cell sequencing technology, the understanding of the composition of cell populations and the interactions between different kinds of cells within skeletal muscle has gradually expanded. Here, the biological meanings of the interaction of muscle satellite cells with the niche cells on the growth, metabolism and regeneration of skeletal muscle has been reviewed, with the aim to provide new ideas to improve the growth rate and meat quality of meat-producing animals.

Key words: skeletal muscle, single-cell sequencing, muscle satellite cells, cell communication

CLC Number:

S852.2

CAO Guancong, MA Lu, REN Lingzhi, LI Yang, SHI Xin'e, YANG Gongshe, LI Xiao. Explore the "Cross-talk" between Skeletal Muscle Satellite Cells and the Niche Cells Based on Single-cell Sequencing Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5340-5348.

Figures/Tables 2

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测序技术 Method	测序细胞数 Cell number	cDNA覆盖度 cDNA coverage	扩增方式 Amplification method	唯一分子标识符 UMI	发表年份 Published year	细胞分选技术 Isolation strategy	参考文献 Reference
Smart-seq	1 000以下	全cDNA序列	PCR	×	2012	FACS	[20]
Smart-seq2	1 000以下	全cDNA序列	PCR	×	2013	FACS	[21]
Cel-seq	1 000以下	3′端	PCR	×	2012	FACS	[22]
cel-seq2	1 000以下	全cDNA序列	IVT	√	2016	FACS	[23]
Drop-seq	高通量	3'端	PCR	√	2015	Microdroplets	[24]
inDrop-seq	高通量	3'端	IVT	√	2015	Microdroplets	[25]
10×Genomics	高通量	3'端	PCR	√	2017	Microdroplets	[26]

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Explore the "Cross-talk" between Skeletal Muscle Satellite Cells and the Niche Cells Based on Single-cell Sequencing Technology

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