C5a-C5aR轴在鸭疫里氏杆菌感染致鸭肝损伤中的作用

doi:10.11843/j.issn.0366-6964.2022.10.036

Abstract

Abstract: This experiment aimed to clarify the role of C5a-C5aR axis in the liver injury of ducklings caused by Riemerella anatipestifer (R. anatipestifer) infection. The preserved R. anatipestifer was resuscitated and duck anticoagulant blood was also prepared. The whole blood experiments were conducted by mixing R. anatipestifer with duck anticoagulant blood and then incubating with anti-C5a antibody and anti-C5aR antibody, respectively. ELISA was used to detect the expression levels of C5a and various inflammatory factors. Then the ducklings were infected with R. anatipestifer and treated with anti-C5a antibody and anti-C5aR antibody, respectively to conduct the animal experiments. The main tissues of ducklings were collected to conduct the histopathological examination. Peripheral blood and liver tissues of ducklings were collected for bacterial counts. Peripheral blood was collected to separate the serum for serum enzyme activity measurement. ELISA was used to detect the expression levels of C5a and inflammatory factors in the serum. The liver tissues were collected to detect the mRNA transcriptional levels of main inflammatory factors by fluorescent quantitative PCR. The results showed that in the whole blood experiments and animal experiments, compared with R. anatipestifer group, the expression levels of C5a, TNF-α, IL-6 and IL-1β in R. anatipestifer + anti-C5a group and R. anatipestifer + anti-C5aR group were significantly decreased (P<0.05). Blocking the C5a-C5aR axis could significantly reduce the morbidity and mortality of ducklings infected with R. anatipestifer, and meanwhile alleviated the tissue damage of the heart, liver and spleen. Blocking the C5a-C5aR axis could significantly reduce the number of bacteria in the peripheral blood and liver tissues, and decreased the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of the ducklings infected with R. anatipestifer. The results of fluorescence quantitative PCR showed that compared with R. anatipestifer group, the mRNA transcriptional levels of C5aR, Sphk1, FGL2, TNF-α and IL-1β in R. anatipestifer + anti-C5a and R. anatipestifer + anti-C5aR groups were significantly decreased (P<0.05). The study successfully confirmed that the activation of C5a-C5aR axis could help R. anatipestifer infect ducklings, and blocking the C5a-C5aR axis could significantly alleviate the tissue damages and inflammatory responses of ducklings, and provided new ideas for further research on anti-R. anatipestifer infection drugs.

Key words: C5a-C5aR axis, Riemerella anatipestifer, complement system, liver injure

CLC Number:

S855.1

LI Delong, WANG Siyuan, YANG Yunchuan, LIU Hongyi, HE Yixiao, LIU Jinlin, GAO Jiye, LI Jixiang, CHEN Sihuai. Role of C5a-C5aR Axis in Liver Injury of Ducklings Caused by Riemerella anatipestifer Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3654-3666.

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Role of C5a-C5aR Axis in Liver Injury of Ducklings Caused by Riemerella anatipestifer Infection

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