猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析

doi:10.11843/j.issn.0366-6964.2020.09.019

Abstract

Abstract: The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared. In this study, the C. suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C. suis were conducted by iTRAQ labeling and LC-MS/MS. Part of the iTRAQ results were validated by qRT-PCR. Total 174 proteins were identified, among which 40 proteins were differentially expressed, 38 proteins were upregulated and 2 proteins were downregulated. The differentially expressed proteins were mainly involved in metabolism, antibiotics biosynthesis, secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways. The results of this study provide reference for elucidating the developmental mechanism of C. suis sporulated oocyst and facilitated discovering a new biomarker for C. suis.

Key words: Cystoisospora suis, sporulated oocyst, unsporulated oocyst, iTRAQ

CLC Number:

S852.723

YANG Shoushen, SUN Xiaoshuang, QIU Yunfei, YANG Fei, TANG Jie, LIAN Wenjian, HUANG Cuiqin, CHIU Hungchuan. Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2216-2226.

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Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis

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