线粒体tRNA-Lys(T7719G)基因变异对绵羊产羔数的影响及遗传学分析

doi:10.11843/j.issn.0366-6964.2022.06.010

Abstract

Abstract: The aim of the present study was to investigate the effect of mitochondrial tRNA-Lys(T7719G) gene variation on the litter size in sheep population with Small-tailed Han sheep as female parent, and to explore the possibility of mitochondrial tRNA-Lys(T7719G) gene variation as molecular marker for assisted selection. Healthy and suitable breeding ewe with Small tail-Han sheep as female parent were selected. The polymorphism analysis of tRNA-Lys(T7719G) gene was conducted by sequencing of PCR amplificatin product, genetic analysis of the influence of tRNA-Lys (T7719G) gene, season and parity on the litter size were conducted. The results showed that the mitochondrial tRNA-Lys gene mutated into G and T alleles, with allele frequency of 57.8% and 42.2%, respectively. The average litter size of ewes with G allele was 0.08 more than that of ewes with T allele in average litter size. Litter size of population increased with the number of parities and the number of individual ewes with G allele in the same season. The formula of prediction model of litter size was established: Litter size = 1.201 + (－0.009)×dummy variable 1 + 0.043×dummy variable 2 + 0.194×dummy variable 3 + 0.061×parity + 0.123×ewes number carrying G allele, which indicated that adding an individual with G allele in population, the litter size was expected to increase 0.123. tRNA-Lys(T7719G) gene can be used as a molecular marker for litter size traits of population with Small-tailed Han sheep as female parent.

Key words: mitochondrion, tRNA-Lys gene, sheep, litter size, genetic analysis

CLC Number:

S826.2

Lü Shiqi, MA Xiaofei, LI Bing, JIA Chunhui, WANG Yanchao, TIAN Shujun, ZHOU Rongyan, CHEN Xiaoyong. Genetics Analysis and Effects of Mitochondrial tRNA-Lys (T7719G) Variation on Litter Size of Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1759-1767.

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Genetics Analysis and Effects of Mitochondrial tRNA-Lys (T7719G) Variation on Litter Size of Sheep

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