患呼吸道疾病羊鼻腔及生活环境微生物多样性分析

doi:10.11843/j.issn.0366-6964.2023.08.030

Abstract

Abstract: This experiment was conducted to understand the microbial diversity of the nasal cavity of sheep with respiratory disease and the surface deposits of their house, and analyze the relationship between the respiratory diseases of sheep and the microbial community composition of their environment. Our study is expected to provide references for prevention and treatment of sheep respiratory diseases. We collected the nasal swabs of sheep and the surface deposits of their house from a sheep farm in Yinchuan, Ningxia. Then, control with nasal swabs and surface deposits of the healthy sheep, 16S rRNA gene sequencing were performed, and the microbial community composition of the nasal cavity and the environment of healthy sheep with respiratory disease were analyzed by bioinformatics. The results showed that the diversity and richness of microbial flora in the nasal cavity and their environment of sheep with respiratory diseases were reduced compared with those healthy sheep. The microbial community composition of the nasal cavity in healthy sheep was significantly different from those sheep with respiratory diseases (P<0.01). Stenotrophomonas is the main genus in the nasal cavity of healthy sheep. Moraxella, Bergeyella and Filobacterium are the predominant genus in the nasal cavity of sheep suffering from respiratory diseases. The microbial community composition in the surface deposits of house between the healthy and the sheep with respiratory diseases also had a significant difference (P<0.05). Firmicutes was the most prominent phyla in diseased sheep. It is concluded that the microbial community composition and relative abundance were changed in the nasal cavity and their environment of sheep with respiratory diseases, but the environment microbial community is not directly related to the respiratory disease of sheep. The imbalance of microbial flora and the increased relative abundance of bacterial pathogens such as Moraxella, Bergeyella, and Filobacterium in diseased sheep may be important factors leading to respiratory diseases in sheep.

Key words: respiratory disease of sheep, nasal microbial community, surface deposits of sheep house, diversity

CLC Number:

S852.6

ZHANG Ying, JIN Hua, HAN Yang, SUI Dan, XIAO Xin, HAO Xiujing, LI Min. Analysis of Microbial Diversity in Nasal Cavity of Sheep with Respiratory Disease and Their Environment[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3455-3465.

References 35

[1]	REAGAN K L, SYKES J E. Canine infectious respiratory disease[J]. Vet Clin North Am Small Anim Pract, 2020, 50(2):405-418.
[2]	PESSOA J, RODRIGUES DA COSTA M, GARCÍA MANZANILLA E, et al. Managing respiratory disease in finisher pigs:combining quantitative assessments of clinical signs and the prevalence of lung lesions at slaughter[J]. Prev Vet Med, 2021, 186:105208.
[3]	CUMMINGS D B, MEYER N F, STEP D L. Bovine respiratory disease considerations in young dairy calves[J]. Vet Clin North Am Food Anim Pract, 2022, 38(1):93-105.
[4]	IQBAL YATOO M, RAFFIQ PARRAY O, TAUSEEF BASHIR S, et al. Contagious caprine pleuropneumonia-a comprehensive review[J]. Vet Quart, 2019, 39(1):1-25.
[5]	SCOTT P R. Treatment and control of respiratory disease in sheep[J]. Vet Clin North Am Food Anim Pract, 2011, 27(1):175-186.
[6]	吴艳茹, 陈巧玲, 刘志勇, 等. 多杀性巴氏杆菌脂多糖刺激羊支气管上皮细胞后的转录组测序与分析[J]. 中国预防兽医学报, 2022, 44(7):704-711, 717.WU Y R, CHEN Q L, LIU Z Y, et al. Transcriptome sequencing and analysis of goat bronchial epithelial cells stimulated by Pasteurella multocida Lipopolysaccharide[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(7):704-711, 717. (in Chinese)
[7]	EWERS C, LUBKE-BECKER A, WIELER L H. Mannheimia haemolytica and the pathogenesis of enzootic bronchopneumonia[J]. Berl Munch Tierarztl Wochenschr, 2004, 117(3-4):97-115. (in German).
[8]	MAO L, YANG L L, LI W L, et al. Epidemiological investigation and phylogenetic analysis of caprine parainfluenza virus type 3 in sheep of China[J]. Transbound Emerg Dis, 2019, 66(3):1411-1416.
[9]	LUO H, WU X, XU Z, et al. NOD2/c-Jun NH2-terminal kinase triggers Mycoplasma ovipneumoniae-induced macrophage autophagy[J]. J Bacteriol, 2020, 202(20):e00689-19.
[10]	TIMSIT E, WORKENTINE M, VAN DER MEER F, et al. Distinct bacterial metacommunities inhabit the upper and lower respiratory tracts of healthy feedlot cattle and those diagnosed with bronchopneumonia[J]. Vet Microbiol, 2018, 221:105-113.
[11]	MANNA S, MCAULEY J, JACOBSON J, et al. Synergism and antagonism of bacterial-viral coinfection in the upper respiratory tract[J]. mSphere, 2022, 7(1):e0098421.
[12]	ZHANG L D, FORST C V, GORDON A, et al. Characterization of antibiotic resistance and host-microbiome interactions in the human upper respiratory tract during influenza infection[J]. Microbiome, 2020, 8(1):39.
[13]	SHOBO C O, ALISOLTANI A, ABIA A L K, et al. Bacterial diversity and functional profile of microbial populations on surfaces in public hospital environments in South Africa:a high throughput metagenomic analysis[J]. Sci Total Environ, 2020, 719:137360.
[14]	ZENDOIA I I, BARANDIKA J F, HURTADO A, et al. Analysis of environmental dust in goat and sheep farms to assess Coxiella burnetii infection in a Q fever endemic area:Geographical distribution, relationship with human cases and genotypes[J]. Zoonoses Public Health, 2021, 68(6):666-676.
[15]	THIBEAULT C, SUTTORP N, OPITZ B. The microbiota in pneumonia:from protection to predisposition[J]. Sci Transl Med, 2021, 13(576):eaba0501.
[16]	GAETA N C, LIMA S F, TEIXEIRA A G, et al. Deciphering upper respiratory tract microbiota complexity in healthy calves and calves that develop respiratory disease using shotgun metagenomics[J]. J Dairy Sci, 2017, 100(2):1445-1458.
[17]	CASADEI E, SALINAS I. Comparative models for human nasal infections and immunity[J]. Dev Comp Immunol, 2019, 92:212-222.
[18]	NICOLA I, CERUTTI F, GREGO E, et al. Characterization of the upper and lower respiratory tract microbiota in Piedmontese calves[J]. Microbiome, 2017, 5(1):152.
[19]	CHURCH D L, CERUTTI L, GURTLER A, et al. Performance and application of 16S rRNA gene cycle sequencing for routine identification of bacteria in the clinical microbiology laboratory[J]. Clin Microbiol Rev, 2020, 33(4):e00053-19.
[20]	WILMANSKI T, RAPPAPORT N, EARLS J C, et al. Blood metabolome predicts gut microbiome α-diversity in humans[J]. Nat Biotechnol, 2019, 37(10):1217-1228.
[21]	ALEXANDER T W, TIMSIT E, AMAT S. The role of the bovine respiratory bacterial microbiota in health and disease[J]. Anim Health Res Rev, 2020, 21(2):168-171.
[22]	TAI J H, HAN M S, KWAK J, et al. Association between microbiota and nasal mucosal diseases in terms of immunity[J]. Int J Mol Sci, 2021, 22(9):4744.
[23]	CHAI J, CAPIK S F, KEGLEY B, et al. Bovine respiratory microbiota of feedlot cattle and its association with disease[J]. Vet Res, 2022, 53(1):4.
[24]	WELP A L, BOMBERGER J M. Bacterial community interactions during chronic respiratory disease[J]. Front Cell Infect Microbiol, 2020, 10:213.
[25]	RYAN R P, MONCHY S, CARDINALE M, et al. The versatility and adaptation of bacteria from the genus Stenotrophomonas[J]. Nat Rev Microbiol, 2009, 7(7):514-525.
[26]	BROOKE J S. Advances in the microbiology of Stenotrophomonas maltophilia[J]. Clin Microbiol Rev, 2021, 34(3):e0003019.
[27]	MCMULLEN C, ALEXANDER T W, LÉGUILLETTE R, et al. Topography of the respiratory tract bacterial microbiota in cattle[J]. Microbiome, 2020, 8(1):91.
[28]	LÓPEZ-SERRANO S, GALOFRÉ-MILÀ N, COSTA-HURTADO M, et al. Heterogeneity of Moraxella isolates found in the nasal cavities of piglets[J]. BMC Vet Res, 2020, 16(1):28.
[29]	董文龙, 魏菁, 耿昕颖, 等. 山羊源腔隙莫拉菌的分离与鉴定[J]. 中国兽医科学, 2015, 45(12):1231-1235.DONG W L, WEI J, GENG X Y, et al. Isolation and identification of Moraxella lacunata from goat[J]. Chinese Veterinary Science, 2015, 45(12):1231-1235. (in Chinese)
[30]	LI F X, ZHU P, LI Z H, et al. Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease[J]. Int J Syst Evol Microbiol, 2022, 72(9):005511.
[31]	LORENZO DE ARRIBA M, LOPEZ-SERRANO S, GALOFRE-MILA N, et al. Characterisation of Bergeyella spp. isolated from the nasal cavities of piglets[J]. Vet J, 2018, 234:1-6.
[32]	NA AČG ERADSKÁ M, PEKOVA S, DANESI P, et al. A novel Filobacterium sp can cause chronic bronchitis in cats[J]. PLoS One, 2021, 16(6):e0251968.
[33]	ZHANG Y, SHEN F X, YANG Y, et al. Insights into the profile of the human expiratory microbiota and its associations with indoor microbiotas[J]. Environ Sci Technol, 2022, 56(10):6282-6293.
[34]	GAO C X, LI Y G, WEI J J, et al. Multi-route respiratory infection:when a transmission route may dominate[J]. Sci Total Environ, 2021, 752:141856.
[35]	VALERIS-CHACIN R, SPONHEIM A, FANO E, et al. Relationships among fecal, air, oral, and tracheal microbial communities in pigs in a respiratory infection disease model[J]. Microorganisms, 2021, 9(2):252.

Analysis of Microbial Diversity in Nasal Cavity of Sheep with Respiratory Disease and Their Environment

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 35

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LONG Tanghui, ZHAN Yanbo, LIAO Guanxiang, CHEN Xinfeng, ZHANG Jian, LI Yanjiao, OUYANG Kehui, QIU Qinghua. Effects of Dietary Lysine Supplementation on Fecal Fermentation Parameters and Microbial Flora Structure of Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2042-2049.
[2]	SONG Kelin, YAN Zunqiang, WANG Pengfei, CHENG Wenhao, LI Jie, BAI Yaqin, SUN Guohu, GUN Shuangbao. Analysis on Genetic Diversity and Genetic Structure Based on SNP Chips of Huixian Qingni Black Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 995-1006.
[3]	YANG Qing, GONG Jing, ZHAO Xueyan, ZHU Xiaodong, GENG Liying, ZHANG Chuansheng, WANG Jiying. Comparison of Array and Resequencing in Pig Genetic Structure Studies [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2772-2782.
[4]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[5]	WU Yicheng, RAN Tao, ZHOU Chuanshe, TAN Zhiliang. Evaluation of the Viral Community Composition in Goat Rumen Fluid, Based on Metagenomic Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2932-2941.
[6]	ZHAO Zhenjian, WANG Shujie, CHEN Dong, JI Xiang, SHEN Qi, YU Yang, CUI Shengdi, WANG Junge, CHEN Ziyang, TANG Guoqing. Population Structure and Genetic Diversity Analysis of Neijiang Pigs Based on Low-coverage Whole Genome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2297-2307.
[7]	TAO Xuan, YANG Xuemei, LIANG Yan, LIU Yihui, WANG Yong, KONG Fanjing, LEI Yunfeng, YANG Yuekui, WANG Yan, AN Rui, YANG Kun, Lü Xuebin, HE Zhiping, GU Yiren. Analysis of Genetic Structure of Conservation Population in Yacha Pig Based on SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2308-2319.
[8]	YANG Sukun, DONG Yimeng, WANG Hongliang, ZHAO Xitang, CHEN Xu, XING Xiumei. Genetic Diversity Analysis of Stud Tahe Red Deer Based on the Gene Fragments of mtDNA and Y Chromosome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2402-2413.
[9]	LONG Xi, CHEN Li, WU Pingxian, ZHANG Tinghuan, PAN Hongmei, ZHANG Liang, WANG Jinyong, GUO Zongyi, CHAI Jie. Evaluation of the Genetic Structure and Selection Signatures in Hechuan Black Pigs Conserved Population [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1854-1867.
[10]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[11]	LIU Jiankui, XU Ye, LIU Chen, YU Hui, YANG Yuan, HE Le, LI Jiarui, DAN Huijuan, DAI Ailing, YANG Xiaoyan, WEI Chunhua. Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in Fujian Province from 2017 to 2021 based on Whole Genome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1579-1589.
[12]	CHEN Yexin, XIE Mengyuan, LI Wenhao, ZHANG Zhidan, WANG Xiaodan, CHEN Kejia, LIU Pingping, ZHOU Weiguang, WANG Jianlong, XU Xiaojing. Analysis of Intestinal Flora in Faecal Samples from Rotavirus and Shigella Positive Calves with Diarrhoea [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1624-1631.
[13]	YAN Qiongxian, CHEN Wenxun, HUI Haoyang, PENG Can, TANG Shaoxun, ZHOU Xiaoling, TAN Zhiliang. Effects of Zearalenone on Growth Performance, Gastrointestinal Fermentation and Microbiota Community Structure of Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1109-1123.
[14]	GAO Chaoqun, CAO Ranran, DU Wenping, HU Xiaoyu, LEI Yanru, LI Wenting, KANG Xiangtao. Genetic Diversity and Population Structure Analysis of Chinese Native Chicken Breeds using Genome-wide SNPs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 554-562.
[15]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.