猪METTL3基因表达水平与DON诱导IPEC-J2细胞损伤的关系

doi:10.11843/j.issn.0366-6964.2022.03.003

Abstract

Abstract: This study aimed to investigate the relationship between the expression level of porcine m⁶A methylases METTL3 and deoxynivalenol (DON) induced injury of intestinal porcine epithelial cells (IPEC-J2). In this study, IPEC-J2 cell lines with METTL3 knockdown was successfully established. IPEC-J2 cells from the METTL3 interference group and control group were treated with 1 μg·mL^-1 DON for 48 h, respectively. Real-time quantitative PCR was used to detect the mRNA expression differences of apoptosis-related genes, immune-related genes and oxidase-related genes, and cell proliferation activity, cell cycle, cell apoptosis, and reactive oxygen species (ROS) levels were measured. The results showed that, compared with control group, the expression of immune-related genes TNF-α, IL-6 and IL-12 in the interference group changed significantly (P<0.05); The levels of apoptosis-related genes Bax, Caspase3 and Caspase9 were significantly decreased(P<0.05); The levels of oxidase-related genes GPXs, CuZn-SOD and CAT were significantly increased(P<0.05). The cell proliferation activity was significantly increased, and the total cell apoptosis rate was significantly decreased (P<0.05); The level of ROS was extremely significantly decreased (P<0.01). The results indicate that the down-regulation expression of METTL3 gene contributes to the enhancement of IPEC-J2 cells resisting apoptosis and inflammation induced by DON in a certain extent. The down-regulation expression of METTL3 gene is beneficial to alleviate a series of inflammatory responses and cell damage caused by DON, and will provide a theoretical basis for in-depth study of the mechanism of RNA methylation in the regulation of piglet resistance to DON damage in the future.

Key words: pigs, METTL3, deoxynivalenol

CLC Number:

S828.2

XU Xie, SHI Dongfeng, WU Jiayun, WU Shenglong, WANG Haifei, WU Zhengchang, BAO Wenbin. Relationship between Expression Level of Porcine METTL3 Gene and the Injury of IPEC-J2 Cells Induced by DON[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 688-699.

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Relationship between Expression Level of Porcine METTL3 Gene and the Injury of IPEC-J2 Cells Induced by DON

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