1株感染多种致病性大肠杆菌噬菌体的生物学特性研究

doi:10.11843/j.issn.0366-6964.2022.01.028

Abstract

Abstract: This study aimed to determine and characterize the biological and infection characteristics of an Escherichia coli phage C6 which was previous isolated from feacal samples of pre-weaned piglet. Bacteriophage classification was confirmed by TEM observation and sequencing. Host range was determined by spot assay and double-layer agar plates method. Infecting characteristics including Optimal multiplicity of infection (MOI), efficiency of plating (EOP), adsorption rate, one-step growth curve as well as bacteriostatic curves of phage C6 were determined. Results were as follows: Escherichia coli phage C6 was a T4-like phage. Phage C6 infected a Escherichia coli O157 strain (E. coli W1), two Shiga toxin-producing Escherichia coli strains (STEC W3, STEC W5) and a enteropathogenic Escherichia coli strain (EPEC 143). The results of each infection index were shown in descending order as follows. Plaque size and EOP: E. coli W1>EPEC 143>STEC W3>STEC W5; optimal MOI: EPEC 143, STEC W3, STEC W5>E. coli W1; EOP and adsorption rate: E. coli W1>EPEC 143, STEC W3, STEC W5; phage proliferation and bacteriostatic efficacy in LB medium: E. coli W1>STEC W3>EPEC 143, STEC W5. E. coli phage C6 was a T4-like phage that infected different pathogenic E. coli strains and had significant bacteriostatic effects on pathogenic E. coli strains despite of varied infection characteristics.

Key words: Escherichia coli phage, Escherichia coli O157, Shiga toxin-producing Escherichia coli, enteropathogenic Escherichia coli, infection characteristics, bacteriostatic effect

CLC Number:

Q939.48

LIN Yan, LUO Runbo, LIU Yankun, ZHU Weiyun. Comparison of Infection Characteristics of an Escherichia coli Phage C6 on Different Pathogenic Escherichia coli Strains[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 282-289.

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Comparison of Infection Characteristics of an Escherichia coli Phage C6 on Different Pathogenic Escherichia coli Strains

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