猪肠道大肠杆菌噬菌体对产肠毒素大肠杆菌的抑菌作用

doi:10.11843/j.issn.0366-6964.2020.07.027

Abstract

Abstract: Previously 57 Escherichia coli phages were isolated and identified from the intestines of healthy pigs, and 12 of them were found to lyse enterotoxigenic Escherichia coli (ETEC). To gain a deep insight of the protective effect of the 12 coliphages on the animal hosts, we determined the host range and bacteriostatic efficacy of the 12 coliphages. 89 E.coli strains (including five ETEC strains) isolated from pig feces previously were used as indicator strains for host spectrum determination. The host range of coliphages was conducted by the spot test method, double-layer agar plate method and liqud LB culture. Bacteriostatic ability of coliphages on ETEC was evaluated by turbidity measurement. The results showed that all the 12 coliphages could lyse one or more ETEC strains, however, none could produce progeny by infecting ETEC. Nevertheless, seven coliphages (S143_1, S143_2, S144_2, S35, S86_1, L86 and S172) could significantly inhibit the growth of ETEC at MOI of 10 or 100. TEM observation revealed that 8 of the 12 coliphages were T4-like coliphages and the other four belonged to Myoviridae. These results suggest that the coliphages isolated from porcine gut which could not infect ETEC but lyse ETEC still inhibit growth of ETEC.

Key words: coliphages, lysis spectrum, enterotoxigenic Escherichia coli, bacteriostatic effect

CLC Number:

Q939.48

ZHOU Bei, LIN Yan, ZHU Weiyun. Study on the Bacteriostatic Effect of Porcine Coliphages on Enterotoxigenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1748-1755.

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Study on the Bacteriostatic Effect of Porcine Coliphages on Enterotoxigenic Escherichia coli

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