西藏羊蜱蝇中巴尔通体检测及gltA基因序列分析

doi:10.11843/j.issn.0366-6964.2020.05.023

Abstract

Abstract: This study aimed to clarify the infection of the pathogenic Bartonella in the ectoparasite Melophagus ovinus in sheep in parts of Tibet. From January to September 2019,298 sheep parasites in Linzhi, Xigaze and Naqu were collected. The morphological identification and PCR amplification of the 18S rRNA gene of the Melophagus ovinus were used to identify the body and the pathogen gltA gane of Bartonella was detected, pMD^TM-18T was connected to some positive PCR products and transferred into DH5α competent cells. The positive results were sequenced and analyzed for genetic and evolutionary analysis. The results showed that the positive rate of female and male were 49.8% (113/227) and 42.3%(30/71), there was no significant difference between male and female(χ²=0.944, P=0.267). The total infection rate of the pathogen Bartonella was 48.0% (143/298). Positive rate of Linzhi was significantly higher than that of Xigaze and Naqu area(χ²=13.801, P<0.01; χ²=17.067,P<0.01), there was no significant difference between Xigaze and Naqu areas(χ²=0.084, P=0.771).The positive rate of Melophagus ovinus in free culture, captivity, and slaughterhouse were 44.3% (102/230), 85.0% (34/40) and 23.3% (7/30), respectively. There were significant differences between captive culture and free host culture (χ²=20.929, P<0.01)or slaughterhouse (χ²=24.38,P<0.01).There was significant differences between host free culture and slaughterhouse(χ²=3.989,P=0.046).The sequencing results were uploaded to GenBank database, and obtained three Bartonella gltA gene accession numbers, MN623006, MN623007 and MN623008. The sequence alignment showed that the homology of Bartonella in Yunnan and Xinjiang was 99.6%-100%. In this study, the pathogen Bartonella of Tibetan Melophagus ovinus was detected for the first time, which provided the basis for understanding the pathogen Bartonella carried by Tibetan sheep parasites in vitro and the prevention and control of the pathogen.

Key words: Tibet, Melophagus ovinus, Bartonella, gltA gene, gene evolution analysis

CLC Number:

ZHOU Saisai, XIE Taifeng, QIAN Wenxian, WANG Yifei, LI Tianjiao, ZHU Jiaping, LUO Runbo, GONG Ga, GESANG Zhuoma, SUOLANG Sizhu. Detection of Bartonella and Sequence Analysis of gltA Gene in Tibetan Melophagus ovinus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1119-1125.

References 32

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Detection of Bartonella and Sequence Analysis of gltA Gene in Tibetan Melophagus ovinus

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