猪ADAR2基因全长cDNA克隆、序列特征及表达模式分析

doi:10.11843/j.issn.0366-6964.2021.03.006

Abstract

Abstract: This study aimed to clone the full-length cDNA of the porcine ADAR2 gene and explore its expression pattern in different tissues of pigs. Rapid-amplification of cDNA ends (RACE) was used to clone the full-length cDNA sequence of the ADAR2 gene in Large White pigs and the sequence was analyzed by bioinformatics. Real-time PCR was used to detect the ADAR2 mRNA expression in the heart, liver, lung, kidney, spleen, brain, small intestine, muscle and backfat of 35-day-old pigs. Porcine ADAR2 cDNA sequence of 6 305 bp was cloned, which contained 12 exons and encoded 704 amino acids. The nucleic acid and amino acid sequences shared a high identity (>84%) with that of other mammals including human, chimpanzee, macaque, gibbon, cow, goat and sheep. The deduced ADAR2 had two double-stranded RNA binding motifs and an adenosine deaminase domain. Real-time PCR results showed that the ADAR2 expressed in all the detected tissues, and had the highest expression in the lung. The full-length cDNA sequence of porcine ADAR2 gene was successfully cloned, and it was widely expressed in tissues of pigs. These findings provide a solid foundation for further function study of ADAR2.

Key words: ADAR2, expression, cDNA clone, RACE, swine

CLC Number:

S828.2

ZHANG Yuebo, WANG Ligang, HOU Xinhua, LIU Xin, YAN Hua, ZHANG Longchao, WANG Lixian. The Full-length cDNA Cloning, Sequence Characterization and Expression Pattern Analysis of Porcine ADAR2 Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 620-629.

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The Full-length cDNA Cloning, Sequence Characterization and Expression Pattern Analysis of Porcine ADAR2 Gene

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