贮精能力差异母鸡的贮精腺形态、激素水平及激素受体基因表达量的研究

doi:10.11843/j.issn.0366-6964.2020.08.008

Abstract

Abstract: The aim of this study was to explore the differences in sperm storage tubules morphology, hormone concentration, and hormone receptor genes expression in hens with divergent sperm storage ability, and to further reveal the mechanism underlying sperm storage. A total of 158 White Leghorn laying hens and 28 rosters of 27 weeks of age were used in this study. After the artificial insemination with mixed semen for two consecutive days, the eggs were collected for each laying hen starting from the 3rd day for 21 days and incubated. According to the fertilization status of the eggs within 21 days after insemination, the individual fertilization rate was counted as the hen's sperm storage ability. The top 4 hens with the highest sperm storage ability and the 4 with the lowest sperm storage ability were selected and named high and low sperm storage ability groups, respectively. The concentrations of progesterone, estrogen, testosterone, and prolactin hormone in serum were detected. The uterine-vaginal junction area of the 8 laying hens was dissected and divided into two parts in the longitudinal direction. One was made into paraffin sections and HE stained for the morphology observation, and the other one was used to detect the expression of the corresponding hormone receptor genes using qRT-PCR assay. The results showed that there was no significant difference in mucosal area of uterine-vaginal junction, sperm storage tubules numbers and density of hens between high and low sperm storage ability groups (P>0.05). The average cross section area of sperm storage tubules of hens in high sperm storage ability group was higher than that of the low sperm storage ability group (P<0.05). The concentration of progesterone in serum of hens in high sperm storage ability group was higher than that of hens in low sperm storage ability group (P<0.05), but there was no significant difference in concentration of estrogen, testosterone or prolactin in serum between groups (P>0.05). Compared with the low sperm storage ability group, hens in the high sperm storage ability group had up-regulated expression of testosterone and prolactin receptor genes, and down-regulated expression of estrogen α/β receptor and progesterone receptor genes, but the differences were not significant (P>0.05). In conclusion, the cross section area of sperm storage tubules may be related to the sperm storage ability of laying hens; in addition, progesterone hormone may play an important role in sperm activation in the sperm storage tubules, which affects the hen's ability to continuous fertilization.

Key words: hen, sperm storage ability, sperm storage tubules, hormone, hormone receptor

CLC Number:

S831.3

MA Tenghe, FAN Jing, WANG Panlin, SHI Lei, LI Yunlei, ADAMU Mani Isa, NI Aixin, GE Pingzhuang, JIANG Linlin, ZONG Yunhe, MA Hui, SUN Yanyan, CHEN Jilan. The Sperm Storage Tubules Morphology, Hormones Concentration, and Hormone Receptor Genes Expression of Laying Hens with Divergent Sperm Storage Ability[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1845-1852.

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The Sperm Storage Tubules Morphology, Hormones Concentration, and Hormone Receptor Genes Expression of Laying Hens with Divergent Sperm Storage Ability

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