近交陆川猪的遗传稳定性研究

doi:10.11843/j.issn.0366-6964.2020.08.003

Abstract

Abstract: The aim of this study was to explore the genetic stability of high-inbreeding Luchuan pig. Ear samples were collected from 20 inbreeding and 6 non-inbreeding Luchuan pigs and genomic re-sequencing was performed for each samples. Genetic diversity of inbreeding Luchuan pig population was analyzed by scanning the whole genome SNPs. The specific ROHs and associated genes existed in inbreeding Luchuan pig population were detected based on genomic homozygosity. The results showed that observed heterozygosity was 0.373 in inbreeding Luchuan pig population, which was less than expected heterozygosity (0.491), indicating that selection or inbreeding might happen in the population. The analysis of phylogenetic trees and population's genetic structure indicated that two inbred lines were generated during the inbreeding process of Luchuan pig and the number 230, 236, 239, 132, 130 and 126 individuals had closer relationship. In inbreeding population, the average of genomic homozygosity and ratio of genomic run of homozygosity were 59.64% and 0.12, respectively, which were extremely significantly higher than 53.18% and 0.05 in non-inbreeding Luchuan pig population (P<0.01), which indicated that the inbreeding Luchuan pig population had reached a high level of inbreeding. One thousand two hundred and fifty and 633 ROHs were found in inbreeding and non-inbreeding Luchuan pig populations, respectively. The results of overlapping analysis suggested that 224 pROHs were specifically existed in inbreeding population. Among them, homozygous mutations generated from inbreeding process might affect 1 322 genes, which involved in hormone biosynthetic process, pancreatic secretion, membrane lipid metabolic process, intestinal immune network for IgA production and Striated muscle cell differentiation. These results indicate that the inbreeding Luchuan pig population has lower genetic diversity, higher genomic homozygosity and reaches a high inbreeding level, which illustrate that the population has high genetic stability. Moreover, the homozygous mutations generated from inbreeding process might be associated with immune, growth and other production traits.

Key words: Luchuan pig, runs of homozygosity, genomic homozygosity, production traits

CLC Number:

S828.2

WANG Hongyang, ZHANG Yingying, TU Weilong, WU Huali, CAO Jianguo, TAN Yongsong. Genetic Stability of Inbreeding Luchuan Pig[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1795-1803.

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Genetic Stability of Inbreeding Luchuan Pig

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