噬菌体降低铜绿假单胞菌所致腐蛋的效果

doi:10.11843/j.issn.0366-6964.2020.07.028

Abstract

Abstract: Pseudomonas aeruginosa is the main pathogen causing rotten eggs during incubation, we attempted to control it with lytic phage. In this work, a strain of virulent phage MH12-Q was sifted from the river water sample by using Pseudomonas aeruginosa PA-MH12 isolated from the rotten eggs. The biological characteristics of the phage were studied and its prevention and control effect on Pseudomonas aeruginosa induced rotten egg were tested. The phage MH12-Q was sequenced and identified as Myoviridae. The titer of phage MH12-Q can reach 10¹³pfu·mL^-1, the complete lysis concentration (CLC) of phage MH12-Q is 10¹⁰pfu·mL^-1, and the optimal growth temperature is 37℃. The optimum pH is 6-8 for the stability of the phage and its titer can maintain a high level at pH 4-10. Its optimal multiplicity of infection (OMOI) is 0.001, the incubation period of infected hosts is 30 min, and the outbreak period is 70 min. The phage with the biological properties above can reduce the infection rate of high concentration Pseudomonas aeruginosa from 70% to 40%. The biological properties of Pseudomonas aeruginosa phage MH12-Q are suitable for production and application, which has a good prevention and control effect on rotten eggs in hatchery and can improve the survival rate of chicken embryos infected with Pseudomonas aeruginosa.

Key words: Pseudomonas aeruginosa, rotten eggs, lytic phage, biological property, prevention and control

CLC Number:

LIU Xiaowen, ZHANG Qing, SONG Xinhui, LUO Yanbo, HU Ming, LI Lulu, QI Jing, ZHANG Yin, ZHAO Xiaonan, LIU Yuqing. Effect of Phage on the Reduction of Rotten Eggs Caused by Pseudomonas aeruginosa[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1756-1763.

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Effect of Phage on the Reduction of Rotten Eggs Caused by Pseudomonas aeruginosa

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