2018年伪狂犬病病毒的流行特征及其遗传变异分析

doi:10.11843/j.issn.0366-6964.2020.03.018

Abstract

Abstract: This experiment was conducted to study the epidemiological and phylogenetic characteristics of swine pseudorabies virus (PRV) currently circulating in China. A total of 1 328 samples from pigs with suspected PRV infection were collected from 28 provinces of China in 2018 for PRV-gE detection by PCR and virus isolation; DNA sequencing was also performed on the gB, gC and gE genes of the PRVs isolated herein and the sequences were used for DNA alignments as well as phylogenetic analysis. The results showed that:1) A total of 92 samples collected herein were positive for PRV-gE by PCR, with an average positive detection rate of 6.93%. Thirteen PRV strains were finally isolated from these 92 PRV-gE positive samples; 2) Phylogenetic analysis on either gB, gC and/or gE showed that the 13 PRVs isolated herein were close to the epidemic PRV strains in China after 2012 but were not close to those circulating in China before 2012; most of the PRV strains isolated from China were located on a different evolutionary branch with those from the other countries such as NIA-3, Bartha, and Kaplan; 3) There were so many amino acid changes with the gB, gC and/or gE proteins in the 13 PRVs isolated herein compared to those in the viruses isolated from the other countries as well as those isolated from China before 2012. These results indicated that the PRVs currently circulating in China were mainly the variant strains that showed different phylogenetic and genetic characteristics from those prevalent in China before 2012 as well as those circulating in the other countries, representing a serious problem.

Key words: pseudorabies virus, PCR detection, virus isolation, phylogenetic analysis, pigs

CLC Number:

S852.659.1

SUN Ying, WANG Xueying, LIANG Wan, XIE Sisi, PENG Zhong, CHEN Hongjian, HUA Lin, SONG Wenbo, TANG Xibiao, CHEN Huanchun, WU Bin. Epidemiological and Evolutionary Characteristics of Pseudorabies Virus in China in 2018[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 584-593.

References 30

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Epidemiological and Evolutionary Characteristics of Pseudorabies Virus in China in 2018

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