一株羊源尸毒梭菌的鉴定及生物学特性研究

doi:10.11843/j.issn.0366-6964.2020.02.022

Abstract

Abstract: To explore the biological characteristics of Clostridium cadaveris isolated from goat, physiological and biochemical analysis, 16S rDNA gene amplification, animal infection test, drug-resistant phenotype and genetic testing for drug resistance of LJH-1 strain were carried out. The results showed that strain LJH-1 was Gram-positive bacteria. A fragment of 1 023 bp was obtained by PCR amplification. LJH-1 and Clostridium cadaveris were clustered, so it was identified as Clostridium cadaveris. All mice in the experimental group died. There were many red blood cells and inflammatory cells in the central hepatic lobular vein of the mice. Blood vessels in the renal stroma were congested. Intestinal mucosa epithelial cells shed. LJH-1 was sensitive to cefotaxime and amoxicillin, and resistant to cefradine and amoxicillin. Ant(3″)-Ia, aac(3)-IIa, aph(3')-IIa, Sul2, Sul3 and TEM were detected. This provided scientific data for the detection, prevention and treatment of the bacterial diseases.

Key words: Clostridium cadaveris, identification, pathogenicity, drug resistance

CLC Number:

S852.61

DOU Pengpeng, WEI Yong, WANG Li. Identification and Biological Characteristics of Clostridium cadaveris Isolated from Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 399-403.

References 19

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Identification and Biological Characteristics of Clostridium cadaveris Isolated from Goat

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