钻孔法增加犬半月板脱细胞基质支架孔隙率的可行性分析

doi:10.11843/j.issn.0366-6964.2020.01.020

Abstract

Abstract: In order to increase the porosity of the meniscus acellular matrix (MAM) scaffolds and improve the amount of cells implanted in them, canine menisci were drilled firstly and then decellularized in this experiment. Next, the decellularization effect and changes in porosity and mechanical properties of the scaffolds were detected. The amount of cells implanted and expression of glycosaminoglycan and type Ⅱ collagen in the scaffolds were examined after chondrogenic induction with BMSCs (bone marrow mesenchymal stem cells). The results showed that the decellularization effect of the drilled scaffold was good, no nuclei were found, and the collagen fibers were structurally intact. The porosity of the scaffold was significantly increased, but the compressive modulus did not change significantly. The amount of BMSCs implanted in the drill scaffold was significantly increased. The rate of cell proliferation and the ability to differentiate into cartilage were significantly improved. The results indicated that the drilling method used can increase the porosity of the meniscus matrix scaffold, which not only maintains the mechanical properties of the scaffold and increases the amount of cells implanted, but also promotes cell proliferation, differentiation and tissue remodeling. It laid a foundation for the wide application of MAM scaffolds in tissue engineering meniscus construction.

Key words: drilling method, meniscus acellular matrix, porosity, canine

CLC Number:

S857.16

ZOU Tong, ZHAO Wen, Lü Yang'ou, GAO Dengke, RUAN Chenmei, ZHANG Xia, ZHANG Yihua. Feasibility Analysis of Drilling Method to Increase Porosity of Canine Meniscus Acellular Matrix Scaffolds[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 179-186.

References 23

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Feasibility Analysis of Drilling Method to Increase Porosity of Canine Meniscus Acellular Matrix Scaffolds

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