SIRT1对牦牛卵母细胞体外成熟与老化的影响

doi:10.11843/j.issn.0366-6964.2019.12.008

Abstract

Abstract: The purpose of this study was to explore the role of SIRT1 on the in vitro maturation and aging of yak oocytes. The SIRT1 specific agonist (SRT2104, SRT group) and inhibitor (Inauhzin, INZ group) were added to the in vitro maturation medium of yak oocytes, respectively. After 24 h in vitro culture of yak cumulus-oocyte complexes (COCs), the expansion of cumulus cells and the excretion of the first polar body were observed. The ROS level in oocyte was measured by immunofluorescence after 24 and 36 h in vitro culture. The expression levels of SIRT1, FOXO3a, SOD2 and Bax in oocytes were detected by RT-qPCR method. Then, yak oocytes were fertilized after 24 and 36 h in vitro culture, and the cleavage and blastocyst formation rates were observed, respectively. The results showed that, after 24 h culture, the cumulus cells expansion degree in SRT group was significantly higher than those in the control group (P<0.05), while the cumulus cells expansion degree and the first polar body excretion rate in INZ group were significantly lower than those in the control group (P<0.05). With the increasing of culture time, the ROS level in oocyte increased significantly (P<0.05); SRT2104 addition significantly inhibited the ROS accumulation in oocytes (P<0.05), while Inauhzin addition significantly increased the ROS level in oocytes (P<0.05). After 24 h culture, the expressions of SIRT1, FOXO3a and SOD2 in SRT group were significantly higher than those in the control group (P<0.05), but the expression of Bax was significantly lower (P<0.05); the expressions of SIRT1, FOXO3a and SOD2 in INZ group were significantly lower than those in the control group (P<0.05), but the expression of Bax was significantly increased (P<0.05). After 24 h culture, the cleavage rate and blastocyst formation rate of oocytes in SRT group were significantly higher than those in INZ group and control group (P<0.05). While after 36 h culture, the cleavage rate and blastocyst formation rate of oocytes in INZ group were significantly lower than those in other groups (P<0.05). In conclusion, SIRT1 is involved in regulating in vitro maturation of yak oocytes. Adding SIRT1 agonist properly in in vitro culture medium can promote in vitro maturation and alleviate aging of yak oocytes, simultaneously improve the developmental capacity of early embryos.

Key words: SIRT1, oocyte, maturation, aging, yak

CLC Number:

S823.85

XIONG Xianrong, WANG Yan, LI Jian, XIONG Yan, ZI Xiangdong, QIU Xiang. Effects of SIRT1 on the in vitro Maturation and Aging of Yak Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2440-2448.

References 29

[1]	兰道亮,熊显荣,位艳丽,等.基于RNA-Seq高通量测序技术的牦牛卵巢转录组研究:进一步完善牦牛基因结构及挖掘与繁殖相关新基因[J].中国科学:生命科学,2014,44(3):307-317.LAN D L,XIONG X R,WEI Y L,et al.RNA-Seq analysis of yak ovary:improving yak gene structure information and mining reproduction-related genes[J].Science China:Life Sciences,2014,44(3):307-317.(in Chinese)
[2]	BEI W L, ZHANG X H.Nucleus or cytoplasm? The mysterious case of SIRT1's subcellular localization[J].Cell Cycle,2016, 15(24):3337-3338.
[3]	TATONE C,DI EMIDIO G,VITTI M,et al.Sirtuin functions in female fertility:possible role in oxidative stress and aging[J].Oxid Med Cell Longev, 2015,2015:659687.
[4]	ARUL NAMBI RAJAN K,KHATER M,SONCIN F,et al.Sirtuin1 is required for proper trophoblast differentiation and placental development in mice[J].Placenta, 2018,62:1-8.
[5]	KOJIMA Y A,ASSYLBEKOVA B,ZHAO B H,et al.Morphoproteomics identifies the EZH2 and SIRT1 pathways as potential blocks to differentiation in yolk sac tumor of the ovary and provides therapeutic options:a case study[J].Ann Clin Lab Sci, 2017,47(1):88-91.
[6]	DOU X W,SUN Y,LI J Z,et al.Short-term rapamycin treatment increases ovarian lifespan in young and middle-aged female mice[J].Aging Cell,2017,16(4):825-836.
[7]	ZHANG J J,FANG L,LU Z Y,et al.Are sirtuins markers of ovarian aging?[J].Gene,2016,575:680-686.
[8]	LIU M Y,YIN Y,YE X Y,et al.Resveratrol protects against age-associated infertility in mice[J].Hum Reprod,2013,28(3):707-717.
[9]	KHAN I,KIM S W,LEE K L,et al.Polydatin improves the developmental competence of bovine embryos in vitro via induction of sirtuin 1(Sirt1)[J].Reprod Fert Develop,2017,29(10):2011-2020.
[10]	SATO D,ITAMI N,TASAKI H,et al.Relationship between mitochondrial DNA copy number and SIRT1 expression in porcine oocytes[J].PLoS One,2014,9(4):e94488.
[11]	SANTOS L,ESCANDE C,ESCANDE A.Potential modulation of sirtuins by oxidative stress[J].Oxid Med Cell Longev,2016, 2016:9831825.
[12]	田长永,杨景晁,马泽芳,等.卵丘细胞对辽宁绒山羊卵母细胞体外成熟与孤雌发育的影响[J].畜牧兽医学报,2010,41(5):543-548.TIAN C Y,YANG J C,MA Z F,et al.Effects of cumulus cells on in vitro maturation and parthenogenetic development of Liaoning cashmere goats oocytes[J].Acta Veterinaria et Zootechnica Sinica,2010,41(5):543-548.(in Chinese)
[13]	FERNANDES H,CASTRO F C,SCHEFER L,et al.Effect of melatonin and its receptors on bovine oocyte maturation and cumulus cell gene expression after heat shock in vitro:preliminary results[J].Reprod Fert Develop,2018,31(1):206-207.
[14]	LEE S,JIN J X,TAWEECHAIPAISANKUL A,et al.Sonic hedgehog signaling mediates resveratrol to improve maturation of pig oocytes in vitro and subsequent preimplantation embryo development[J].J Cell Physiol,2018,233(6):5023-5033.
[15]	WANG F,TIAN X Z,ZHANG L,et al.Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization[J].Fertil Steril,2014,101(2):577-586.e1.
[16]	RIEPSAMEN A,WU L,LAU L,et al.Nicotinamide impairs entry into and exit from meiosis I in mouse oocytes[J].PLoS One,2015,10(5):e0126194.
[17]	BORRADAILE N M,PICKERING J G.Polyploidy impairs human aortic endothelial cell function and is prevented by nicotinamide phosphoribosyltransferase[J].Am J Physiol Cell Physiol,2010,298(1):C66-C74.
[18]	THAKER R,MISHRA V,GOR M,et al.The role of stimulation protocol,number of oocytes retrieved with respect to follicular fluid oxidative stress and IVF outcome[J].Hum Fert,2019,doi:10.1080/14647273.2018.1551630.
[19]	HORI Y S,KUNO A,HOSODA R,et al.Regulation of FOXOs and p53 by SIRT1 modulators under oxidative stress[J].PLoS One,2013,8(9):e73875.
[20]	ZHANG T,CHI Y Q,REN Y Z,et al.Resveratrol reduces oxidative stress and apoptosis in podocytes via sir2-related enzymes,Sirtuins1(SIRT1)/peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) axis[J].Med Sci Monit,2019, 25:1220-1231.
[21]	SONG S B,HWANG E S.A rise in ATP,ROS,and mitochondrial content upon glucose withdrawal correlates with a dysregulated? mitochondria turnover mediated by the activation of the protein deacetylase SIRT1[J].Cells,2019,8(1):11.
[22]	DI EMIDIO G,FALONE S,VITTI M,et al.SIRT1 signalling protects mouse oocytes against oxidative stress and is deregulated during aging[J].Hum Reprod,2014,29(9):2006-2017.
[23]	CAITO S,RAJENDRASOZHAN S,COOK S,et al.SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by oxidants and carbonyl stress[J].FASEB J,2010,24(9):3145-3159.
[24]	PECK B,CHEN C Y,HO K K,et al.SIRT inhibitors induce cell death and p53 acetylation through targeting both SIRT1 and SIRT2[J]. Mol Cancer Ther,2010,9(4):844-855.
[25]	LORD T,MARTIN J H,AITKEN R J.Accumulation of electrophilic aldehydes during postovulatory aging of mouse oocytes causes reduced fertility,oxidative stress,and apoptosis[J].Biol Reprod,2015,92(2):1-13.
[26]	SUN S C,GAO W W,XU Y N,et al.Degradation of actin nucleators affects cortical polarity of aged mouse oocytes[J].Fertil Steril,2012,97(4):984-990.
[27]	ZHENG J,YIN X Q,GE W,et al.Post-ovulatory aging of mouse oocytes in vivo and in vitro:effects of caffeine on exocytosis and translocation of cortical granules[J].Anim Sci J,2016,87(11):1340-1346.
[28]	ZHAO S,LIU Z X,AO Z J,et al.Age-associated potency decline in bovine oocytes is delayed by blocking extracellular Ca2+ influx[J].Theriogenology,2015,83(9):1493-1501.
[29]	DEMYDA-PEYRÁS S,DORADO J,HIDALGO M,et al.Effects of oocyte quality,incubation time and maturation environment on the number of chromosomal abnormalities in IVF-derived early bovine embryos[J].Reprod Fertil Dev,2013,25(7):1077-1084.

Effects of SIRT1 on the in vitro Maturation and Aging of Yak Oocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 29

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[2]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[3]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[4]	LI Zaiwen, LI Xiang, LI Xiaowei, LI Biao, JIANG Mingfeng. The Pedigree Reconstruction of the Maiwa Yak Preserved Population Based on GBS Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3710-3721.
[5]	SHEN Yingchao, DAVSHILT Toli, REN Hong, WANG Xisheng, TIAN Shuyue, DU Ming, DUGARJAVIIN Manglai, BOU Gerelchimeg. Differential Expression of Oocyte Development-related Hormone and Growth Factor Receptors in Equine Expanded and Compact Cumulus-oocyte Complexes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3735-3744.
[6]	XU Xi, YANG Baigao, ZHANG Hang, FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3348-3357.
[7]	WANG Jingyu, PAN Yangyang, XU Gengquan, ZHANG Rui, ZHANG Wenlan, WANG Xiaoshan, WU Rentaodi, ZHAO Rigetu, CUI Yan, YU Sijiu. Preparation and Preliminary Application of Yak (Bos Grunniens) Fas-associated Factor 1 Polyclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3369-3382.
[8]	ZHANG Hang, YANG Baigao, XU Xi, FENG Xiaoyi, DU Weihua, HAO Haisheng, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Research Progress on the Mechanism of Heat Stress Affecting the Development of Dairy Cow Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2692-2700.
[9]	ZHANG Peng, WANG Mingxiu, JING Kemin, PENG Wei, TIAN Yuan, LI Yuqian, FU Changqi, SHU Shi, ZHONG Jincheng, CAI Xin. Abnormal Expression of FGFs/FGFRs and Their Mediated Signaling Pathway Genes Affect the Proliferative Activity of Undifferentiated Spermatogonia in Cattleyak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2886-2897.
[10]	JIAO Zhengxing, PAN Yangyang, WANG Meng, WANG Jinglei, MA Wenbin, GAO Xiang, ZHANG Hui, CUI Yan, YU Sijiu, WANG Libin. Preparation of Polyclonal Antibody against Yak LC3B Protein and Its Application in Detection of Expression in Reproductive Organs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2436-2447.
[11]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[12]	ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.
[13]	ZHANG Peipei, HAO Haisheng, DU Weihua, ZHU Huabin, LI Shujing, YU Wenli, ZHAO Xueming. A Review of Optimization of in vitro Maturation System of OPU Oocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1359-1369.
[14]	XIAO Shiyu, LU Chang, MA Juan, WANG Chuang, QI Meiyu, YAO Yuchang. Effects of N-acetylcysteine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1046-1057.
[15]	MENG Zhaoyi, WANG Yunlu, XU Yefen, NIU Jiaqiang, SUOLANG Sizhu, GUO Min, XI Guangyin. Construction of Yak lncRNA ENSBGRT00000000387.1 Lentivirus Vector and Its Effect on Apoptosis of Yak Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1058-1070.