牦牛SIRT3基因的克隆及其在各组织和睾丸不同发育阶段的表达

doi:10.11843/j.issn.0366-6964.2019.12.007

Abstract

Abstract: This research was conducted to clone the sirtuin 3 (SIRT3) gene in yak and to identify its expression pattern in various tissues and testis at different development ages, respectively. This research might provide reliable basis for studying the mechanism of SIRT3 in testis development of yak. A total of 12 healthy male yaks (3 in each group) in fetal (5-6 months), calve (1-2 years old), sexual maturity (3-5 years old) and old (7-9 years old) stages were selected. Three samples from the same age were considered as biological duplications. The samples of liver, heart, spleen, lung, brain, kidney, muscle, small intestine, testicle and large intestine were collected from yaks during sexual maturity stage, and testicles were collected from yaks at 4 different development stages, respectively. Total RNAs in different samples were extracted respectively and the coding sequence of yak SIRT3 gene was cloned by reverse transcription PCR(RT-PCR). The structure and function of the protein encoded by SIRT3 gene were predicted by related bioinformatics softwares. The expressions of SIRT3 mRNA in different tissues and testis at 4 different development stages were determined by quantitative real-time PCR (qRT-PCR). The results showed that the ORF(open reading frame) of SIRT3 was 1 002 bp, which encoded 333 amino acids. The sequence of SIRT3 showed 99.9% and 96.7% homology with that of cattle and sheep, respectively. The mRNA of SIRT3 was widely expressed in various tissues of yak, and its expression was relatively higher in spleen, testis, lung and muscle. The expression of SIRT3 mRNA in the testis of yak increased firstly and then decreased with age, with the highest expression in sexual maturity stage. This study provides basic data for revealing the regulation of SIRT3 in yak growth and development, especially in testicular development.

Key words: histone deacetylation, SIRT3, yak, tissue expression pattern, testis

CLC Number:

S823.85

WANG Bin, TONG Lianxin, QIN Wenchang, LI Jian, YIN Shi. Cloning of Yak SIRT3 Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2431-2439.

References 33

[1]	SHEN Y,WEI W,ZHOU D X.Histone acetylation enzymes coordinate metabolism and gene expression[J].Trends Plant Sci,2015,20(10):614-621.
[2]	YIN S,JIANG X H,JIANG H W,et al.Histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating reactive oxygen species levels[J].Development,2017,144(12):2165-2174.
[3]	MILLARD C J,WATSON P J,FAIRALL L,et al.Targeting class I histone deacetylases in a "Complex" environment[J]. Trends Pharmacol Sci,2017,38(4):363-377.
[4]	ALVES-FERNANDES D K,JASIULIONIS M G.The role of SIRT1 on DNA damage response and epigenetic alterations in cancer[J].Int J Mol Sci,2019,20(13):3153.
[5]	ZHOU L,XU D Y,SHA W G,et al.Long non-coding RNA MALAT1 interacts with transcription factor Foxo1 to regulate SIRT1 transcription in high glucose-induced HK-2 cells injury[J].Biochem Biophys Res Commun, 2018, 503(2):849-855.
[6]	MAHMUD Z,GOMES A R,LEE H J,et al.EP300 and SIRT1/6 co-regulate lapatinib sensitivity via modulating FOXO3-acetylation and activity in breast cancer[J].Cancers,2019,11(8):1067
[7]	BOTTA G,DE SANTIS L P,SALADINO R.Current advances in the synthesis and antitumoral activity of SIRT1-2 inhibitors by modulation of p53 and pro-apoptotic proteins[J].Curr Med Chem,2012,19(34):5871-5884.
[8]	KOSCIUK T,WANG M,HONG J Y,et al.Updates on the epigenetic roles of sirtuins[J].Curr Opin Chem Biol, 2019, 51:18-29.
[9]	KATWAL G,BARAL D,FAN X L,et al.SIRT3 a major player in attenuation of hepatic ischemia-reperfusion injury by reducing ROS via its downstream mediators:SOD2,CYP-D,and HIF-1α[J].Oxid Med Cell Longev,2018, 2018:2976957.
[10]	STORDER J,RENARD P,ARNOULD T.Update on the role of Sirtuin 3 in cell differentiation:a major metabolic target that can be pharmacologically controlled[J].Biochem Pharmacol,2019,169:113621.
[11]	CUI X X,LI X,DONG S Y,et al.SIRT3 deacetylated and increased citrate synthase activity in PD model[J].Biochem Biophys Res Commun,2017,484(4):767-773.
[12]	LIU X H,ZHANG L,WANG P,et al.Sirt3-dependent deacetylation of SOD2 plays a protective role against oxidative stress in oocytes from diabetic mice[J].Cell Cycle,2017,16(13):1302-1308.
[13]	YU W,DITTENHAFER-REED K E,DENU J M.SIRT3 protein deacetylates isocitrate dehydrogenase 2(IDH2) and regulates mitochondrial redox status[J].J Biol Chem,2012,287(17):14078-14086.
[14]	PARODI-RULLÁN R M,CHAPA-DUBOCQ X R,JAVADOV S.Acetylation of mitochondrial proteins in the heart:the role of SIRT3[J].Front Physiol,2018,9:1094.
[15]	WEI L,ZHOU Y,QIAO C,et al.Oroxylin a inhibits glycolysis-dependent proliferation of human breast cancer via promoting SIRT3-mediated SOD2 transcription and HIF1α destabilization[J].Cell Death Dis,2015,6(4):e1714.
[16]	权凯.牦牛超数排卵技术研究[D].兰州:甘肃农业大学,2004.QUAN K.Study on superovulation of yak[D].Lanzhou:Gansu Agricultural University,2004.(in Chinese)
[17]	蔡雯祎,熊显荣,陈通,等.KDM2B基因克隆及其在牦牛组织和卵母细胞减数分裂过程中的表达研究[J].畜牧兽医学报, 2018,49(3):534-541.CAI W Y,XIONG X R,CHEN T,et al.Molecular cloning of KDM2B gene and its expression pattern in tissues and the process of oocyte meiosis in yak[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):534-541.(in Chinese)
[18]	阮崇美.不同发育期白牦牛睾丸蛋白质组学分析及生殖相关候选基因HSP60生物学研究[D].兰州:甘肃农业大学,2017.RUAN C M.Proteomic analysis of white yak testis in different developmental stages and reproductive candidate gene HSP60 biology research[D].Lanzhou:Gansu Agricultural University,2017.(in Chinese)
[19]	SALVATORI I,VALLE C,FERRI A,et al.SIRT3 and mitochondrial metabolism in neurodegenerative diseases[J].Neurochem Int,2017,109:184-192.
[20]	QUAN Y Z,WANG N T,CHEN Q Q,et al.SIRT3 inhibits prostate cancer by destabilizing oncoprotein c-MYC through regulation of the PI3K/Akt pathway[J].Oncotarget,2015,6(28):26494-26507.
[21]	WEI T,HUANG G J,GAO J,et al.Sirtuin 3 deficiency accelerates hypertensive cardiac remodeling by impairing angiogenesis[J].J Am Heart Assoc,2017,6(8):e006114.
[22]	PAULIN R,DROMPARIS P,SUTENDRA G,et al.Sirtuin 3 deficiency is associated with inhibited mitochondrial function and pulmonary arterial hypertension in rodents and humans[J].Cell Metab,2014,20(5):827-839.
[23]	PENG Y H,YANG C,SHI X L,et al.Sirt3 suppresses calcium oxalate-induced renal tubular epithelial cell injury via modification of FoxO3a-mediated autophagy[J].Cell Death Dis,2019,10(2):34.
[24]	CHEN C J,FU Y C,YU W,et al.SIRT3 protects cardiomyocytes from oxidative stress-mediated cell death by activating NF-κB[J].Biochem Biophys Res Commun,2013,430(2):798-803.
[25]	方妙玉.琼玉膏经MAPK/ERK1/2信号通路延缓大鼠脾脏衰老机制研究[D].广州:广州中医药大学,2016.FANG M Y.Study on Qiongyugao aging mechanisms via MAPK/ERK1/2 signal pathway on the spleen of aging rats[D].Guangzhou:Guangzhou University of Chinese Medicine,2016.(in Chinese)
[26]	CORONA J C,DUCHEN M R.PPARγ and PGC-1α as therapeutic targets in Parkinson's[J].Neurochem Res,2015, 40(2):308-316.
[27]	EDGETT B A,HUGHES M C,MATUSIAK J B L,et al.SIRT3 gene expression but not SIRT3 subcellular localization is altered in response to fasting and exercise in human skeletal muscle[J].Exp Physiol,2016,101(8):1101-1113.
[28]	GUERRIERO G,TROCCHIA S,ABDEL-GAWAD F K,et al.Roles of reactive oxygen species in the spermatogenesis regulation[J].Front Endocrinol (Lausanne),2014,5:56.
[29]	DI-LUOFFO M,BROUSSEAU C,BERGERON F,et al.The transcription factor MEF2 is a novel regulator of gsta gene class in mouse MA-10 leydig cells[J].Endocrinology,2015,156(12):4695-4706.
[30]	BANIHANI S A.Effect of coenzyme Q10 supplementation on testosterone[J].Biomolecules,2018,8(4):172.
[31]	MALMIR M,SOLEIMANI MEHRANJANI M,NADERI NOREINI S,et al.Protective antioxidant effects of N-acetylcysteine against impairment of spermatogenesis caused by paranonylphenol[J]. Andrologia,2018, 50(10):e13114.
[32]	KWON Y,KIM J,LEE C Y,et al.Expression of SIRT1 and SIRT3 varies according to age in mice[J].Anat Cell Biol, 2015,48(1):54-61.
[33]	TATONE C,DI EMIDIO G,VITTI M,et al.Sirtuin functions in female fertility:possible role in oxidative stress and aging[J].Oxid Med Cell Longev,2015,2015:659687.

Cloning of Yak SIRT3 Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 33

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[2]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[3]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[4]	LI Zaiwen, LI Xiang, LI Xiaowei, LI Biao, JIANG Mingfeng. The Pedigree Reconstruction of the Maiwa Yak Preserved Population Based on GBS Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3710-3721.
[5]	WANG Jingyu, PAN Yangyang, XU Gengquan, ZHANG Rui, ZHANG Wenlan, WANG Xiaoshan, WU Rentaodi, ZHAO Rigetu, CUI Yan, YU Sijiu. Preparation and Preliminary Application of Yak (Bos Grunniens) Fas-associated Factor 1 Polyclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3369-3382.
[6]	HU Ting, ZHANG Yonghong, HOU Xiaolin, YAO Hua, CUI Defeng, PAN Zaozao, ZHANG Lingyu, ZHANG Jiaxi, WU Qiong. The Effects of Bisphenol A on Inflammation and Amino Acid Metabolism Pathways in Porcine Testis Sertoli Cells Based on Transcriptome Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2858-2871.
[7]	QING Enhua, TANG Bincheng, NIU Tian, WANG Junqi, CHEN Zhaoyan, HU Jiwei, HE Hua, LI Liang, WANG Jiwen, HU Shenqiang. Effects of Different Dry Rearing Systems on Histomorphology of Goose Testis and External Genitalia [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2872-2885.
[8]	ZHANG Peng, WANG Mingxiu, JING Kemin, PENG Wei, TIAN Yuan, LI Yuqian, FU Changqi, SHU Shi, ZHONG Jincheng, CAI Xin. Abnormal Expression of FGFs/FGFRs and Their Mediated Signaling Pathway Genes Affect the Proliferative Activity of Undifferentiated Spermatogonia in Cattleyak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2886-2897.
[9]	JIAO Zhengxing, PAN Yangyang, WANG Meng, WANG Jinglei, MA Wenbin, GAO Xiang, ZHANG Hui, CUI Yan, YU Sijiu, WANG Libin. Preparation of Polyclonal Antibody against Yak LC3B Protein and Its Application in Detection of Expression in Reproductive Organs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2436-2447.
[10]	SUN Junfeng, PAN Jianqiu, ZHANG Zhuoshen, JIANG Danli, SHENG Xu, CHEN Rong, XU Danning, TIAN Yunbo, HUANG Yunmao. The Expression Patterns of OPN5-TSH-DIO2/DIO3 and VIP-PRL in the Process of Repressing the Testicular Function of Male Shanma Duck under Short-day Photoperiod [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2001-2012.
[11]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[12]	MENG Zhaoyi, WANG Yunlu, XU Yefen, NIU Jiaqiang, SUOLANG Sizhu, GUO Min, XI Guangyin. Construction of Yak lncRNA ENSBGRT00000000387.1 Lentivirus Vector and Its Effect on Apoptosis of Yak Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1058-1070.
[13]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Transcriptome Analysis of the Cerebral Cortex in Newborn and Adult Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4605-4614.
[14]	ZHANG Qiqi, WANG Junmei, YUE Ziqi, GUO Yixin, SHI Liyuan, ZHANG Xiaohong, ZOU Huawei, PENG Quanhui, XUE Bai, WANG Lizhi, WANG Zhisheng, HU Rui. Effect of LPS on the Complement C3 Activation and ATP Production in the Rumen Epithelial Cells of Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4664-4675.
[15]	YANG Baigao, HAO Haisheng, DU Weihua, ZHU Huabin, ZHAO Xueming. Advances in Research on Plateau Adaptation of Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 12-23.