miR-200c和miR-429靶向调节绵羊皮下脂肪细胞中SCD1表达的研究

doi:10.11843/j.issn.0366-6964.2019.07.004

Abstract

Abstract: This study aimed to predict and validate the key miRNAs that regulated SCD1 gene expression in ovine subcutaneous adipocytes. miRNAs targeting SCD1 were predicted by bioinformatics softwares of TargetScan, mirDB, StarBase and miRanda. The dual luciferase reporter system was used to verify the target relationships between SCD1 gene and miRNAs. Fluorescent quantitative PCR and Western-blotting techniques were used to detect the effect of miRNAs on SCD1 expression at mRNA and protein levels. The prediction results indicated that SCD1 gene was targeted by both miR-200c and miR-429. Dual luciferase reporter system assay demonstrated that there were target relationships between the 2 miRNAs and SCD1 gene. Fluorescent quantitative PCR analysis showed that miR-200c and miR-429 significantly inhibited SCD1 mRNA expression (P<0.05), and miR-200c was more effective. Western-blotting results showed that miR-200c and miR-429 reduced SCD1 protein level (P<0.01). Microscopical observation showed that miR-200c and miR-429 inhibited ovine subcutaneous lipid droplet formation. In conclusion, miR-200c and miR-429 can down-regulate SCD1 gene expression, thus inhibit ovine lipogenesis.

CLC Number:

S826.2

WANG Shufang, PAN Yangyang, REN Duanyang, ZHAO Yanyan, LI Baojun, QIAO Liying, LIU Wenzhong. Regulation of SCD1 Gene Expression by miR-200c and miR-429 in Ovine Subcutaneous Adipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1347-1357.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.07.004

https://www.xmsyxb.com/EN/Y2019/V50/I7/1347

References

[1] HEINEMANN F S,OZOLS J.Stearoyl-CoA desaturase,a short-lived protein of endoplasmic reticulum with multiple control mechanisms[J].Prostaglandins Leukot Essent Fatty Acids,2003,68(2):123-133.
[2] DOBRZYN A,NTAMBI J M.The role of stearoyl-CoA desaturase in the control of metabolism[J].Prostaglandins Leukot Essent Fatty Acids,2005,73(1):35-41.
[3] ALJOHANI A M,SYED D N,NTAMBI J M.Insights into stearoyl-CoA desaturase-1 regulation of systemic metabolism[J].Trends Endocrinol Metab,2017,28(12):831-842.
[4] MAULUCCI G,COHEN O,DANIEL B,et al.Fatty acid-related modulations of membrane fluidity in cells:detection and implications[J].Free Radic Res,2016,50(S1):S40-S50.
[5] KAKUMA T,LEE Y,UNGER R H.Effects of leptin,troglitazone,and dietary fat on stearoyl CoA desaturase[J].Biochem Biophys Res Commun,2002,297(5):1259-1263.
[6] GIVEL A M,KIEFFER Y,SCHOLER-DAHIREL A,et al.miR200-regulated CXCL12β promotes fibroblast heterogeneity and immunosuppression in ovarian cancers[J].Nat Commun,2018,9(1):1056.
[7] HYUN S,LEE J H,JIN H,et al.Conserved microRNA miR-8/miR-200 and its target USH/FOG2 control growth by regulating PI3K[J].Cell,2009,139(6):1096-1108.
[8] LIU X L,GAO H X,WANG B C,et al.miR-192-5p regulates lipid synthesis in non-alcoholic fatty liver disease through SCD-1[J].World J Gastroenterol,2017,23(46):8140-8151.
[9] QIANG J,TAO Y F,HE J,et al.miR-29a modulates SCD expression and is regulated in response to a saturated fatty acid diet in juvenile genetically improved farmed tilapia (Oreochromis niloticus)[J].J Exp Biol,2017,220(8):1481-1489.
[10] GUO Y, YU J, WANG C, et al. miR-212-5p suppress lipid accumulation by targeting FAS and SCD1[J]. J Mol Endocrinol, 2017, 59(3):205-217.
[11] CHENG X, XI Q Y, WEI S, et al. Critical role of miR-125b in lipogensis by targeting stearoyl-CoA desaturase-1(SCD1)[J]. J Anim Sci,2016, 94(1):65-76.
[12] LEWIS B P,BURGE C B,BARTEL D P.Conserved seed pairing,often flanked by adenosines,indicates that thousands of human genes are microRNA targets[J].Cell,2005,120(1):15-20.
[13] WONG N,WANG X W.miRDB:an online resource for microRNA target prediction and functional annotations[J].Nucleic Acids Res,2015,43(D1):D146-D152.
[14] LI J H,LIU S,ZHOU H,et al.starBase v2.0:decoding miRNA-ceRNA,miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data[J].Nucleic Acids Res,2014,42(D1):D92-D97.
[15] JOHN B,ENRIGHT A J,ARAVIN A,et al.Human microRNA targets[J].PLoS Biol,2004,2(11):e363.
[16] FENG X L,WANG Z M,FILLMORE R,et al.miR-200,a new star miRNA in human cancer[J].Cancer Lett,2014,344(2):166-173.
[17] UHLMANN S,ZHANG J D,SCHWÄGER A,et al.miR-200bc/429 cluster targets PLCγ1 and differentially regulates proliferation and EGF-driven invasion than miR-200a/141 in breast cancer[J].Oncogene,2010,29(30):4297-4306.
[18] TAO C,REN H Y,XU P,et al.Adipocyte miR-200b/a/429 ablation in mice leads to high-fat-diet-induced obesity[J].Oncotarget, 2016,7(42):67796-67807.
[19] CHARTOUMPEKIS D V,ZARAVINOS A,ZIROS P G,et al.Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice[J].PLoS One,2012,7(4):e34872.
[20] ENDO K,WENG H C,KITO N,et al.miR-216a and miR-216b as markers for acute phased pancreatic injury[J].Biomed Res,2013,34(4):179-188.
[21] GOODWIN D,ROSENZWEIG B,ZHANG J,et al.Evaluation of miR-216a and miR-217 as potential biomarkers of acute pancreatic injury in rats and mice[J].Biomarkers,2014,19(6):517-529.
[22] PAN Y Y,JING J J,QIAO L Y,et al.miR-124-3p affects the formation of intramuscular fat through alterations in branched chain amino acid consumption in sheep[J].Biochem Biophys Res Commun,2018,495(2):1769-1774.
[23] 赵艳艳,乔利英,景炅婕,等.miR-142和miR-144靶向FoxO1基因调节绵羊前体脂肪细胞分化的研究[J].畜牧兽医学报,2018,49(4):675-684.
ZHAO Y Y,QIAO L Y,JING J J,et al.Study on the differentiation regulation of miR-142 and miR-144 on ovine preadipocytes by targeting FoxO1 gene[J].Acta Veterinaria et Zootechnica Sinica,2018,49(4):675-684.(in Chinese)
[24] ZHANG H N,LIU J,QU D,et al.Inhibition of miR-200c restores endothelial function in diabetic mice through suppression of COX-2[J].Diabetes,2016,65(5):1196-1207.
[25] WU T T,ZHOU H B,HONG Y F,et al.miR-30 family members negatively regulate osteoblast differentiation[J].J Biol Chem, 2012, 287(10):7503-7511.
[26] YAO D, LUO J, HE Q, et al. SCD1 alters long-chain fatty acid (LCFA) composition and its expression is directly regulated by SREBP-1 and PPARγ1 in dairy goat mammary cells[J]. J Cell Physiol, 2017,232(3):635-649.
[27] IKEDA J, ICHIKI T, TAKAHARA Y, et al. PPARγ agonists attenuate palmitate-induced ER stress through up-regulation of SCD1 in macrophages[J]. PLoS One, 2015,10(6):e0128546.
[28] CHU K K,MIYAZAKI M,MAN W C,et al.Stearoyl-coenzyme A desaturase 1 deficiency protects against hypertriglyceridemia and increases plasma high-density lipoprotein cholesterol induced by liver X receptor activation[J].Mol Cell Biol,2006, 26(18):6786-6798.
[29] ZHU D X,ZHU W,FANG C,et al.miR-181a/b significantly enhances drug sensitivity in chronic lymphocytic leukemia cells via targeting multiple anti-apoptosis genes[J].Carcinogenesis,2012,33(7):1294-1301.
[30] CHU X L,WANG Y Q,PANG L W,et al.miR-130 aggravates acute myocardial infarction-induced myocardial injury by targeting PPAR-γ[J].J Cell Biochem,2018,119(9):7235-7244.
[31] ILIOPOULOS D,DROSATOS K,HIYAMA Y,et al.microRNA-370 controls the expression of microRNA-122 and Cpt1α and affects lipid metabolism[J].J Lipid Res,2010,51(6):1513-1523.
[32] GUO J,FANG W W,SUN L B,et al.Reduced miR-200b and miR-200c expression contributes to abnormal hepatic lipid accumulation by stimulating JUN expression and activating the transcription of srebp1[J].Oncotarget,2016,7(24):36207-36219.
[33] XU Z H,BU Y W,CHITNIS N,et al.miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis[J].Nat Commun,2016,7:11422.

Regulation of SCD1 Gene Expression by miR-200c and miR-429 in Ovine Subcutaneous Adipocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Yang, YU Qian, LIU Yucheng, YANG Hua, ZHAO Zhuo, WANG Limin, ZHOU Ping, YANG Qingyong, DAI Rong. Identification and Tissue Expression Analysis of the Sheep MYL Gene Family [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1551-1564.
[2]	KANG Jia, DUAN Xiangru, YIN Xuejiao, YANG Ruochen, LI Taichun, SHAN Xinyu, CHEN Meijing, ZHANG Yingjie, LIU Yueqin. Effects of Cysteine and Methionine on Secondary Hair Follicle Growth and Hair Dermal Papilla Cell Proliferation in vitro in Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 515-527.
[3]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[4]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[5]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[6]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[7]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[8]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[9]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[10]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[11]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[12]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[13]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[14]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[15]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.