CRISPR/Cas9基因编辑系统研究进展及其在动物基因编辑研究中的应用

doi:10.11843/j.issn.0366-6964.2016.07.001

Abstract

Abstract:

CRISPR/Cas (Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated(Cas)) system is a DNA modified gene editing tools regulated by a kind of short RNA.This technology is a novel tool，which is more rapid，efficient and accurate than ZFN (zinc-fingers nuclease) and TALEN (transcription activator-like (TAL) effector nucleases) in genetic engineering.It is easy to be designed and has specificity.This paper reviews the structure and function of CRISPR/Cas9 system and the design strategy of Cas9 and its application in animal genetic engineering.CRISPR/Cas9 is successfully used in a variety of animal genetic editing，it is likely to become a new way to establish animal models and to develop a new feasible approach to prevent disease.

CLC Number:

S813.3

WANG Wei-wei，LIU Rui-qi，WU Yong-yan，YANG Yan-ge，WANG Yong-sheng，QING Su-zhu. The Progress of CRISPR/Cas9 System and Its Application in Animal Genetic Engineering[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1299-1305.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.07.001

https://www.xmsyxb.com/EN/Y2016/V47/I7/1299

References

［1］ISHINO Y，SHINAGAWA H，MAKINO K，et al.Nucleotide sequence of the iap gene，responsible for alkaline phosphatase isozyme conversion in Escherichia coli，and identification of the gene product［J］.J Bacteriol，1987，169：5429-5433.
［2］GAJ T，GERSBACH C A，BARBAS C F，et al.ZFN，TALEN，and CRISPR/Cas-based methods for genome engineering［J］. Cell，2013，31：397-405.
［3］MAKAROVA K S，ARAVIND L，GRISHIN N V，et al.A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis［J］.Nucleic Acids Res，2002，30(2)：482-496.
［4］GUY C P，MAJERNÍK A I，CHONG J P，et al.A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system［J］.Nucleic Acids Res，2004，32(21)：6176-6186.
［5］GRISSA I，VERGNAUD G，POURCEL C，et al.The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats［J］.BMC Bioinformatics，2007，8：172.
［6］HSU P D，LANDER E S，ZHANG F，et al.Development and Applications of CRISPR-Cas9 for Genome engineering［J］.Cell，2014，157：1262-1278.
［7］PELLETIER S，GINGRAS S，GREEN D R，et al.Mouse genome engineering via CRISPR-Cas9 for study of immune function［J］.Immunity，2015，42：18-27.
［8］HSU P D，SCOTT D A，WEINSTEIN J A，et al.DNA targeting specificity of RNA-guided Cas9 nucleases［J］.Nat Biotechnol， 2013，31(9)：827-832.
［9］DOUDNA J A，CHARPENTIER E.The new frontier of genome engineering with CRISPR-Cas9［J］.Science，2014，246：1258096(1-9).
［10］RAN F A，HSU P D，WRIGHT J，et al.Genome engineering using the CRISPR-Cas9 system［J］.Nat Protocols，2013，8：2281-2308.
［11］XIAO A，CHENG Z，KONG L，et al.CasOT：a genome-wide Cas9/gRNA off-target searching tool［J］.Bioinformatics，2014，30(8)：1180-1182.
［12］HOCKEMEYER D，WANG H，KIANI S，et al.Genetic engineering of human pluripotent cells using TALEnucleases［J］.Nat Biotechnol，2011，29(8)：731-734.
［13］CONG L，RAN F A，COX D，et al.Multiplex genome engineering using CRISPR/Cas systems［J］.Science，2013，339：819-823.
［14］SHALEM O，SANJANA N E，HARTENIAN E，et al.Genome-scale CRISPR-Cas9 knockout screening in human cells［J］.Science，2014，343：84-87.
［15］MALI P，YANG L，ESVELT K M，et al.RNA-guided human genome engineering via Cas9［J］.Science，2013，339(6121)：823-826.
［16］DIANOV G L，HÜBSCHER U.Mammalian base excision repair：the forgotten archangel［J］.Nucleic Acids Res， 2013，41(6)：3483-3490.
［17］KIM H，KIM J S.A guide to genome engineering with programmable nucleases［J］.Nat Rev Genet，2014，15(5)：321-334.
［18］方锐，畅飞，孙照霖，等.Crispr/Cas9介导的基因组定点编辑技术［J］.生物化学与生物物理进展，2013，40(8)：691-702.
FANG R，CHANG F，SUN Z L，et al.New method of genome editing derived from CRISPR/Cas9［J］.Progress in Biochemistry and Biophysics，2013，40(8)：691-702.(in Chinese)
［19］GAJ T，GERSBACH C A，BARBAS C F.ZFN，TALEN，and CRISPR/Cas-based methods for genome engineering［J］.Trends Biotechnol，2013，31(7)：397-405.
［20］SAPRANAUSKAS R，GASIUNAS G，FREMAUX C，et al.The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli［J］.Nucleic Acids Res，2011，39(21)：9275-9282.
［21］DOW L E，FISHER J，O’ROURKE K P，et al.Inducible in vivo genome editing with CRISPR-Cas9［J］. Nat Biotechnol， 2015，33(4)：390-394.
［22］KLEINSTIVER B P，PREW M S，TSAI S Q，et al.Engineered CRISPR-Cas9 nucleases with altered PAM specificities［J］.Nature，2015，523(7561)：481-485.
［23］JIANG W，BIKARD D，COX D，et al.RNA-guided editing of bacterial genomes using CRISPR-Cas systems［J］. Nat Biotechnol，2013，31(3)：233-239.
［24］CHO S W，KIM S，KIM J M，etal.Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease［J］.Nat Biotechnol，2013，31(3)：230-232.
［25］JINEK M，EAST A，CHENG A，et al.RNA-programmed genome editing in human cells［J］.Elife，2013，2(1)：e00471.
［26］NISHIMASU H，RAN F A，HSU P D，et al.Crystal structure of Cas9 in complex with guide RNA and target DNA［J］.Cell，2014，156(5)：935-949.
［27］CARROLL D.Staying on target with CRISPR-Cas［J］.Nature America，2013，31：807-809.
［28］MONTAGUE T G，CRUZ J M，GAGNON J A，et al.CHOPCHOP：a CRISPR/Cas9 and TALEN web tool for genome editing［J］.Nucleic Acids Res，2014，25：1-7.
［29］WILES M V，QIN W，CHENG A W，et al.CRISPR-Cas9-mediated genome editing and guide RNA design［J］.Mamm Genome，2015，26(9-10)：501-510.
［30］GUSMAO E G，DIETERICH C，ZENKE M，et al.Detection of active transcription factor binding sites with the combination of DNase hypersensitivity and histone modifications［J］.Bioinformatics，2014，30(22)：3143-3151.
［31］RAN F A，HSU P D，LIN C Y，et al.Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity［J］.Cell，2013，154(6)：1380-1389.
［32］BHAKTA M S，SEGAL D J.The generation of zinc finger proteins by modular assembly［J］.Methods Mol Biol，2010，649：3-30.
［33］KIM D，BAE S，PARK J，et al.Digenome-seq：genome-wide profiling of CRISPR-Cas9 off-target effects in human cells［J］.Nat Methods，2015，12(3)：237-243.
［34］CHO S W，KIM S，KIM Y，et al.Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases［J］. Genome Res，2014，24(1)：132-141.
［35］FU Y F，SANDER J D，REYON D，et al.Improving CRISPR-Cas nuclease specificity using truncated guide RNAs［J］.Nat Biotechnol，2014，32(3)：279-284.
［36］TREVINO A E，ZHANG F.Chapter eight-genome editing using Cas9 nickases［J］.Methods Enzymol，2014，546：161-174.
［37］YANG H，WANG H，SHIVALILA C S，et al.One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，153：1370-1379.
［38］WANG H，YANG H，SHIVALILA C S，et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，153：910-918.
［39］NIU Y，SHEN B，CUI Y，et al.Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos［J］.Cell，2014，156：836-843.
［40］CHEN F，WANG Y，YUAN Y，et al.Generation of B cell-deficient pigs by highly efficient CRISPR/Cas9-mediated gene targeting［J］.J Genet Genomics，2015，42(8)：437-444.
［41］TU Z，YANG W，YAN S，et al.CRISPR/Cas9：a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases［J］.Mol Neurodegener，2015，10：35-37.
［42］ZHOU X，XIN J，FAN N，et al.Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer［J］.Cell Mol Life Sci，2015，72(6)：1175-1184.
［43］CRISPO M，MULET A P，TESSON L，et al.Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes［J］.PLoS One，2015，10(8)：366-375.
［44］WANG X，YU H，LEI A，et al.Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system［J］.Sci Rep，2015，5：13878.
［45］YAN Q，ZHANG Q，YANG H，et al.Generation of multi-gene knockout rabbits using the Cas9/gRNA system［J］.Cell Regen(Lond)，2014，3(1)：12.
［46］VÉRON N，QU Z，KIPEN P A，et al.CRISPR mediated somatic cell genome engineering in the chicken［J］.Dev Biol，2015，407(1)：68-74.
［47］KOTANI H，TAIMATSU K，OHGA R，et al.Efficient multiple genome modifications induced by the crRNAs，tracrRNA and Cas9 protein complex in zebrafish［J］.PLoS One，2015，10(5)：128-134.
［48］HRUSCHA A，KRAWITZ P，RECHENBERG A，et al.Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish［J］.Development，2013，140(24)：4982-4987.
［49］WANG Z.Genome engineering in cattle：recent technological advancements［J］.Chromosome Res，2015，23(1)：17-29.
［50］HE X，TAN C，WANG F，et al.Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair［J］.Nucleic Acids Res，2016，［Epub ahead of print］.
［51］ZETSCHE B，GOOTENBERG J S，ABUDAYYEH O O，et al.Cpfl is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system［J］.Cell，2015，163(3)：759-771.
［52］SLAYMAKER I M，GAO L，ZETSCHE B，et al.Rationally engineered Cas9 nucleases with improved specificity［J］.Science，2016，351(6268)：84-88.
［53］JIANG W，ZHOU H，BI H，et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis，tobacco，sorghum and rice［J］.Nucleic Acids Res， 2013，41(20)：188-193.

[1]	LI Chunyan, ZHANG Yan, Lü Chunrong, DENG Weidong, QUAN Guobo. Research Progress on Antioxidant Mechanisms of Melatonin and Its Application in Cryopreservation of Mammalian Spermatozoa [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4468-4476.
[2]	LIU Shuang, HE Lixia, MA Jun, FENG Xue, YANG Mengli, WANG Shuzhe, YANG Runjun, FANG Xibi, XIAN Hailong, WANG Yongkang, ZHANG Lupei, MA Yun. Analysis on Genetic Background and Body Size Indexes and Beef-purpose Index of Guyuan Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2376-2388.
[3]	YANG Sukun, DONG Yimeng, WANG Hongliang, ZHAO Xitang, CHEN Xu, XING Xiumei. Genetic Diversity Analysis of Stud Tahe Red Deer Based on the Gene Fragments of mtDNA and Y Chromosome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2402-2413.
[4]	LIU Ling, WANG Dandan, CUI Kai, MA Yuehui, JIANG Lin. Advances of Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 434-442.
[5]	WANG Honghao, REN Xiaokang, ZHANG Yi, GAO Huijiang, CHE Leijie, WANG Xi. Application of Gene Chip Technology in Jinnan Bull Breeding [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2803-2813.
[6]	LIU Xiaoqian, JIN Lanjie, DONG Yanqiu, LI Dongjie, ZHANG Cui, GU Shukai, LI Shijie. DNA Methylation Regulate the Genomic Imprinting of AQP1 Gene Specific in Bovine Placenta [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2181-2189.
[7]	GUO Xiaoxiao, LI Yinxia, WANG Yue, ZHANG Han, ZHANG Jun, QIAN Yong, MENG Chunhua, WANG Huili, ZHONG Jifeng, CAO Shaoxian. Polymorphisms in the 5'Regulatory Region of PLAG1 Gene and Their Association with Early Body Weight of Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 331-343.
[8]	GUO Jiankang, FAN Ziyao, NIU Pengxia, LIU Zhiguo, MU Yulian, ZHANG Mingrui, LI Kui, WANG Bingyuan. The Study of Ide Gene Regulating Myoblast Proliferation and Differentiation through AKT [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1784-1794.
[9]	YAO Dawei, MA Jing, CHEN Lili, WANG Tianzhen, SUN Huan, SONG Wenqin, MA Yi. Effects of Interfering PTEN Gene on the Transcription of Lipid Synthesis-related Genes and Fatty Acid Composition in Goat Mammary Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 851-860.
[10]	CUI Sheng, LIN Yaqiu, XU Qing, ZHU Jiangjiang, WANG Yong. Interfering Smad3 Promotes Goat Adipocyte Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 475-489.
[11]	MA Jing, YAO Dawei, YANG Chunlei, LI Qiuling, WANG Tianzhen, CHEN Chengbin, SONG Wenqin, MA Yi. Effects of Interfering with CREB Gene on Lipid Synthesis Related Gene Expression and Triacylglycerol Synthesis in Mammary Epithelial Cells of Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2413-2421.
[12]	ZHANG Cui, CHEN Weina, LI Junliang, GU Shukai, XU Da, LI Dongjie, LI Shijie. Abnormal Expression of LINC24065 in Somatic Cell Nuclear Transfer Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 44-51.
[13]	WANG Guan-nan, ZHAO Yu-peng, CHEN Wei-na, ZHANG Cui, XU Da, LI Dong-jie, LI Shi-jie. Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1000-1006.
[14]	LI Li-sha, PENG Yong-dong, ZHENG Xiao-ning, LI Xiang-long. Analysis of the Promoter Activity and Transcriptional Regulatory Elements of Goat PMEL Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 826-835.
[15]	WANG Ji-ying, WANG Yan-ping, XU Yun-hua, WANG Cheng, LIN Hai-chao, HU Hong-mei, WU Ying, GUO Jian-feng. Analyses of Intramuscular Fat Content, Fatty Acid Composition and the Related Traits in Lulai Black Pigs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 585-594.

The Progress of CRISPR/Cas9 System and Its Application in Animal Genetic Engineering

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments