豆状带绦虫Tp18基因的原核表达及其产物的抗原性分析

doi:10.11843/j.issn.0366-6964.2013.11.017

Abstract

Abstract:

In order to evaluate the effect of immunoprotection and diagnosis against rabbit Taenia pisiformis cysticercosis by recombinant Tp18 (rTp18) protein, Tp18 gene was screened from transcriptome of adult T. pisiformis, then was cloned and expressed. rTp18 protein was used as immunogen and atopen. The samples were grouped into three experimental groups, including rTp18 protein group, PBS group and adjuvant group. Immunization was performed twice at 14-day intervals. Seven days after the second immunization, each rabbit was experimentally infected orally with 5 000 mature viable T. pisiformis eggs, and sacrificed at 49 days post-infection. The number of cysticercoids was counted. By the end of the experiment, serum was collected for detection of IgG and IgA every week. Repeated trial of rTp18 protein group and PBS group was carried out using identical procedures and conditions. The molecular weight of rTp18 protein is about 32 kDa. rTp18 protein was proved to be well reacted with the positive sera against T. pisiformis from rabbit by Western blotting. Vaccination trials showed that the rTp18 protein induced effective immune protection against T. pisiformis cysticercosis with 95.59% reduction （97.38% reduction in repeated trial） in metacestode burdens of vaccinated rabbits, which was significantly higher than PBS and adjuvant group (P<0.01). IgG was the antibody of humoral immunity. Meanwhile, dot-ELISA was used to diagnose T. pisiformis cysticercosis for rabbits. Based on the results of rabbit necropsy of 169 abattoir rabbit samples, the relative sensitivity and specificity of the dot-ELISA were 90.74% (49/54) and 96.52% (111/115), respectively. These promising results indicate that rTp18 protein is suitable for the development of effective vaccination and diagnositic strategies against cysticercosis in rabbits, providing a foundation for control of T. pisiformis cysticercosis.

CLC Number:

S852.734

YANG De-ying, CHEN Lin, GU Xiao-bin, LAI Song-jia, SUN Jia-gang,YANG Guang-you. Prokaryotic Expression of Tp18 Gene from Taenia pisiformis and Antigenicity Analysis of the Expressed Product[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1819-1825.

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Prokaryotic Expression of Tp18 Gene from Taenia pisiformis and Antigenicity Analysis of the Expressed Product

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