内皮素-3对羊驼黑色素细胞特征及细胞内毛色基因表达的影响

doi:10.11843/j.issn.0366-6964.2013.07.012

Abstract

Abstract:

The study aimed to investigate the effects of endothelin-3 (EDN3) on the expression of hair color genes of alpaca. The activity of melanocyte, the production of melanin in melanocytes and the expressions of related genes and proteins including Endothelin receptor B (EDNRB), KIT, Microphthalmia-associtated transcription factor (MITF) and Tyrosinase (TYR) in alpaca melanocytes in cultures with addition of different dose of EDN3（0, 10^-9, 10^-8, 10^-7 mol·L^-1）, were examined by MTT, ultraviolet spectrophotometry, qRT-PCR and Western blotting, respectively. The results showed that the amount of melanocytes was increased with the increasing double- or tri- dendrites; At the addition dose of 10^-8 mol·L^-1 for 72 h after adding EDN3, compared to control group, the proliferation of melanocytes was obvious and the expressions of EDNRB, KIT, MITF and TYR significantly up-regulated at the level of both transcription and translation in melanocytes（P<0.05）. Moreover, the production of melanin in melanocytes significantly increased（P<0.05）. These findings indicate that EDN3 play an important role in regulating melanins production in melanocytes in alpaca.

CLC Number:

S813.3

GENG Jian-jun, BAI Jun-ming, FAN Rui-wen, YANG Shan-shan, PANG Ya-miao, DONG Chang-sheng. The Effects of Endothelin-3 on the Melanocyte Characteristic and Expression of Hair Color Genes in Melanocytes from Alpaca (Lama Pacos) in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1070-1077.

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The Effects of Endothelin-3 on the Melanocyte Characteristic and Expression of Hair Color Genes in Melanocytes from Alpaca (Lama Pacos) in vitro

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