基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

doi:10.11843/j.issn.0366-6964.2025.12.022

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (12): 6191-6203.doi: 10.11843/j.issn.0366-6964.2025.12.022

基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

刘彬¹, 朱康平¹, 周程鹏^2,3,4, 周立新⁵, 薛佳⁵, 沈林園^2,3,4, 甘麦邻^2,3,4, 朱砺^2,3,4*

1. 四川德康农牧食品集团股份有限公司农业农村部西南生猪育种与扩繁重点实验室, 成都 610200;
2. 四川农业大学动物科技学院, 猪禽种业全国重点实验室, 成都 611130;
3. 四川农业大学动物科技学院农业农村部畜禽生物组学重点实验室, 成都 611130;
4. 四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室, 成都 611130;
5. 成都市动物疫病预防控制中心, 成都 610041

收稿日期:2025-05-16 发布日期:2025-12-24
通讯作者: 朱砺,主要从事猪遗传育种研究,E-mail:zhuli7508@163.com
作者简介:刘彬(1994-)，男，四川德阳人，硕士，主要从事猪遗传育种研究，E-mail：13018240830@163.com
基金资助:
国家重点研发计划(2021YFD1200801)；四川省科技计划(2021ZDZX0008；2021YFYZ0030)；国家现代农业产业技术体系四川生猪创新团队(SCCXTD-2025-08)；国家生猪产业技术体系(CARS-35)

Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning

LIU Bin¹, ZHU Kangping¹, ZHOU Chengpeng^2,3,4, ZHOU Lixin⁵, XUE Jia⁵, SHEN Linyuan^2,3,4, GAN Mailin^2,3,4, ZHU Li^2,3,4*

1. Key Laboratory of Southwest Pig Breeding and Expansion of Ministry of Agriculture and Rural Affairs, Sichuan Dekang Agriculture, Animal Husbandry and Food Group Co., Ltd., Chengdu 610200, China;
2. National Key Laboratory of Pig and Poultry Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
3. Key Laboratory of Livestock and Poultry Biomics of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
4. Sichuan Provincial Key Laboratory of Exploration and Innovation Utilization of Livestock and Poultry Genetic Resources, Sichuan Agricultural University, Chengdu 611130, China;
5. Chengdu Animal Disease Prevention and Control Center, Chengdu 610041, China

Received:2025-05-16 Published:2025-12-24

摘要/Abstract

摘要： 旨在评估体细胞克隆技术(SCNT)在托佩克E系超级公猪快速扩群中的应用效果。本研究以12头超级公猪为供体，进行细胞分离培养、冻存和扩增传代培养出1 100个胚胎并移植至6头母猪，最终4头分娩29头仔猪(克隆效率2.63%)，并对克隆猪与非克隆猪的生长发育、精液品质及所配母猪繁殖性能进行比较分析。结果，克隆猪与供体正常后代在生长发育上无显著差异，克隆猪在精液体积((188.17±49.27)mL)和有效精子数((486.35±159.56)亿)相较于供体同胞后代和供体半同胞无显著差异(P＞0.05)，配种后的母猪繁殖性能无明显差异，且SNP检测证明克隆猪与供体基因不一致率均在0.14%以下，表明遗传信息来源于供体。综上，克隆猪相较于供体后代繁殖性能和生长性能无明显差异，SCNT技术可有效用于超级公猪扩群生产。

关键词: 体细胞克隆, 种公猪, 扩繁

Abstract: The study aimed to evaluate the application effect of somatic cell cloning technology (SCNT) in the rapid expansion of Topigs E line super boars. Using 12 super boars as donors, 1 100 embryos were isolated, cultured, frozen, amplified and subcultured, and transferred to 6 sows. Finally, 4 sows delivered 29 piglets (cloning efficiency 2.63%). The growth and develo-pment, semen quality and reproductive performance of cloned and non-cloned pigs were compared and analyzed. The results showed that there was no significant difference in growth and development between cloned pigs and donor normal offspring. There was no significant difference in semen volume ((188.17±49.27) mL) and effective sperm number ((486.35±159.56) million) between cloned pigs and donor siblings and donor half-sibs. There was no significant difference in reproductive performance of sows after mating. SNP detection showed that the inconsistency rate between cloned pigs and donor genes was below 0.14%, indicating that genetic information was derived from donors. In summary, there was no significant difference in reproductive performance and growth performance between cloned pigs and donor offspring. SCNT technology can be effectively used for super boar expansion production.

Key words: somatic cell cloning, breeding boars, expanding reproduction

中图分类号:

S828.3

刘彬, 朱康平, 周程鹏, 周立新, 薛佳, 沈林園, 甘麦邻, 朱砺. 基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析[J]. 畜牧兽医学报, 2025, 56(12): 6191-6203.

LIU Bin, ZHU Kangping, ZHOU Chengpeng, ZHOU Lixin, XUE Jia, SHEN Linyuan, GAN Mailin, ZHU Li. Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6191-6203.

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基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning

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