基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

doi:10.11843/j.issn.0366-6964.2025.12.022

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (12): 6191-6203.doi: 10.11843/j.issn.0366-6964.2025.12.022

基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

刘彬¹(), 朱康平¹, 周程鹏²^,³^,⁴, 周立新⁵, 薛佳⁵, 沈林園²^,³^,⁴, 甘麦邻²^,³^,⁴, 朱砺²^,³^,⁴^,*()

1. 四川德康农牧食品集团股份有限公司农业农村部西南生猪育种与扩繁重点实验室, 成都 610200
2. 四川农业大学动物科技学院, 猪禽种业全国重点实验室, 成都 611130
3. 四川农业大学动物科技学院农业农村部畜禽生物组学重点实验室, 成都 611130
4. 四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室, 成都 611130
5. 成都市动物疫病预防控制中心, 成都 610041

收稿日期:2025-05-16 出版日期:2025-12-23 发布日期:2025-12-24
通讯作者: 朱砺 E-mail:13018240830@163.com;zhuli7508@163.com
作者简介:刘彬(1994-)，男，四川德阳人，硕士，主要从事猪遗传育种研究，E-mail：13018240830@163.com
基金资助:
国家重点研发计划(2021YFD1200801)；四川省科技计划(2021ZDZX0008；2021YFYZ0030)；国家现代农业产业技术体系四川生猪创新团队(SCCXTD-2025-08)；国家生猪产业技术体系(CARS-35)

Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning

LIU Bin¹(), ZHU Kangping¹, ZHOU Chengpeng²^,³^,⁴, ZHOU Lixin⁵, XUE Jia⁵, SHEN Linyuan²^,³^,⁴, GAN Mailin²^,³^,⁴, ZHU Li²^,³^,⁴^,*()

1. Key Laboratory of Southwest Pig Breeding and Expansion of Ministry of Agriculture and Rural Affairs, Sichuan Dekang Agriculture, Animal Husbandry and Food Group Co., Ltd., Chengdu 610200, China
2. National Key Laboratory of Pig and Poultry Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
3. Key Laboratory of Livestock and Poultry Biomics of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
4. Sichuan Provincial Key Laboratory of Exploration and Innovation Utilization of Livestock and Poultry Genetic Resources, Sichuan Agricultural University, Chengdu 611130, China
5. Chengdu Animal Disease Prevention and Control Center, Chengdu 610041, China

Received:2025-05-16 Online:2025-12-23 Published:2025-12-24
Contact: ZHU Li E-mail:13018240830@163.com;zhuli7508@163.com

摘要/Abstract

摘要：

旨在评估体细胞克隆技术(SCNT)在托佩克E系超级公猪快速扩群中的应用效果。本研究以12头超级公猪为供体，进行细胞分离培养、冻存和扩增传代培养出1 100个胚胎并移植至6头母猪，最终4头分娩29头仔猪(克隆效率2.63%)，并对克隆猪与非克隆猪的生长发育、精液品质及所配母猪繁殖性能进行比较分析。结果，克隆猪与供体正常后代在生长发育上无显著差异，克隆猪在精液体积((188.17±49.27)mL)和有效精子数((486.35±159.56)亿)相较于供体同胞后代和供体半同胞无显著差异(P＞0.05)，配种后的母猪繁殖性能无明显差异，且SNP检测证明克隆猪与供体基因不一致率均在0.14%以下，表明遗传信息来源于供体。综上，克隆猪相较于供体后代繁殖性能和生长性能无明显差异，SCNT技术可有效用于超级公猪扩群生产。

关键词: 体细胞克隆, 种公猪, 扩繁

Abstract:

The study aimed to evaluate the application effect of somatic cell cloning technology (SCNT) in the rapid expansion of Topigs E line super boars. Using 12 super boars as donors, 1 100 embryos were isolated, cultured, frozen, amplified and subcultured, and transferred to 6 sows. Finally, 4 sows delivered 29 piglets (cloning efficiency 2.63%). The growth and develo-pment, semen quality and reproductive performance of cloned and non-cloned pigs were compared and analyzed. The results showed that there was no significant difference in growth and development between cloned pigs and donor normal offspring. There was no significant difference in semen volume ((188.17±49.27) mL) and effective sperm number ((486.35±159.56) million) between cloned pigs and donor siblings and donor half-sibs. There was no significant difference in reproductive performance of sows after mating. SNP detection showed that the inconsistency rate between cloned pigs and donor genes was below 0.14%, indicating that genetic information was derived from donors. In summary, there was no significant difference in reproductive performance and growth performance between cloned pigs and donor offspring. SCNT technology can be effectively used for super boar expansion production.

Key words: somatic cell cloning, breeding boars, expanding reproduction

中图分类号:

S828.3

刘彬, 朱康平, 周程鹏, 周立新, 薛佳, 沈林園, 甘麦邻, 朱砺. 基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析[J]. 畜牧兽医学报, 2025, 56(12): 6191-6203.

LIU Bin, ZHU Kangping, ZHOU Chengpeng, ZHOU Lixin, XUE Jia, SHEN Linyuan, GAN Mailin, ZHU Li. Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6191-6203.

图/表 17

表 1

图 1

图 2

表 2

表 3

表 4

图 3

图 4

图 5

图 6

表 5

图 7

表 6

表 7

表 8

表 9

表 10

参考文献 40

1	李淞淋. 猪肉产量和消费量均呈现稳中有降趋势进口量减少[J]. 农产品市场, 2025 (9): 31- 32.
	LI S L . Pork production and consumption showed a steady downward trend. Imports decreased[J]. Agricultural Products Market, 2025 (9): 31- 32.
2	田明, 刘娣. 中国生猪育种历史与进程[J]. 中国畜禽种业, 2024, 20 (12): 10-18, 3.
	TIAN M , LIU D . History and process of pig breeding in China[J]. The Chinese Livestock and Poultry Breeding, 2024, 20 (12): 10-18, 3.
3	王洁. 生猪遗传改良育种对提高养殖效益的作用研究[J]. 猪业科学, 2025, 42 (6): 95- 96.
	WANG J . Study on the effect of pig genetic improvement breeding on improving breeding efficiency[J]. Swine Industry Science, 2025, 42 (6): 95- 96.
4	梁志刚. 生猪育种行业当前发展现状及未来展望[J]. 新农业, 2023 (19): 43- 45.
	LIANG Z G . Current development status and future prospects of pig breeding industry[J]. New Agriculture, 2023 (19): 43- 45.
5	蓝杰, 梁史锋. 育种在养猪业发展中的重要性分析[J]. 猪业观察, 2024 (3): 37- 39.
	LAN J , LIANG S F . Analysis of the importance of breeding in the development of pig industry[J]. Pig Industry Observation, 2024 (3): 37- 39.
6	傅衍. 猪的育种技术及其应用[J]. 猪业科学, 2021, 38 (12): 39- 41.
	FU Y . Pig breeding technology and its application[J]. Swine Industry Science, 2021, 38 (12): 39- 41.
7	段永邦. 生猪遗传改良育种与可持续养殖的关系探讨[J]. 猪业科学, 2025, 42 (8): 106- 107.
	DUAN Y B . Discussion on the relationship between pig genetic improvement breeding and sustainable breeding[J]. Swine Industry Science, 2025, 42 (8): 106- 107.
8	孙启峰. 生猪育种存在的问题及育种方式的选择[J]. 畜牧业环境, 2024 (6): 16- 17.
	SUN Q F . Problems in pig breeding and selection of breeding methods[J]. Animal Husbandry Environment, 2024 (6): 16- 17.
9	张琪, 于永生, 张树敏, 等. 我国引进猪种与地方猪种选育过程中的几个关键问题[J]. 猪业科学, 2022, 39 (1): 103- 105.
	ZHANG Q , YU Y S , ZHANG S M , et al. Several key problems in the breeding process of introduced pig breeds and local pig breeds in China[J]. Swine Industry Science, 2022, 39 (1): 103- 105.
10	WELLS D N . Animal cloning: problems and prospects[J]. Rev Sci Tech, 2005, 24 (1): 251- 264. doi: 10.20506/rst.24.1.1566
11	OUYANG H , HAN J , HUANG Y . Pig cloning using somatic cell nuclear transfer[J]. Methods Mol Biol, 2021, 2239, 1- 18.
12	GLANZNER W G , RISSI V B , BORDIGNON V . Somatic cell nuclear transfer in pigs[J]. Methods Mol Biol, 2023, 2647, 197- 210.
13	敖政, 陈祥, 吴珍芳, 等. 体细胞克隆猪发育异常研究进展[J]. 遗传, 2020, 42 (10): 993- 1003.
	AO Z , CHEN X , WU Z F , et al. Research progress on abnormal development of somatic cloned pigs[J]. Hereditas, 2020, 42 (10): 993- 1003.
14	陈海云, 林晓, 滕丽琼, 等. 体细胞克隆及胚胎移植技术在优质基因种猪群快速扩繁中的应用[J]. 兽医导刊, 2022 (1): 159- 160.
	CHEN H Y , LIN X , TENG L Q , et al. Application of somatic cell cloning and embryo transfer technology in rapid propagation of high-quality gene breeding pigs[J]. Veterinary Orientation, 2022 (1): 159- 160.
15	李爱民, 阳文攀, 邓政, 等. 利用体细胞克隆技术扩繁超级公猪的应用价值分析[J]. 中国畜牧杂志, 2024, 60 (10): 50- 54.
	LI A M , YANG W P , DENG Z , et al. Analysis of the application value of somatic cell cloning technology to expand super boars[J]. Chinese Journal of Animal Science, 2024, 60 (10): 50- 54.
16	陈龙, 雷胜辉, 李金龙, 等. 托佩克种猪生长性能、胴体性状及肉质性状测定报告[J]. 今日养猪业, 2022 (3): 97- 101.
	CHEN L , LEI S H , LI J L , et al. Report on the determination of growth performance, carcass traits and meat quality traits of Topeke breeding pigs[J]. Pig Industry Today, 2022 (3): 97- 101.
17	张似青, 王龙钦, 王晓兵, 等. 托佩克(Topigs)种猪繁殖与生长性能分析[J]. 猪业科学, 2020, 37 (5): 110- 113.
	ZHANG S Q , WANG L Q , WANG X B , et al. Analysis of reproductive and growth performance of Topigs breeding pigs[J]. Swine Industry Science, 2020, 37 (5): 110- 113.
18	沈林園, 甘麦邻, 郭芝忺, 等. 体细胞克隆在青峪猪遗传资源保护中的运用效果分析[J]. 中国畜牧杂志, 2021, 57 (S1): 200- 205.
	SHEN L Y , GAN M L , GUO Z X , et al. Analysis of the application effect of somatic cell cloning in the protection of genetic resources of Qingyu pig[J]. Chinese Journal of Animal Science, 2021, 57 (S1): 200- 205.
19	吴亚林, 李继良, 周伟良, 等. 猪体细胞克隆胚胎移植技术要点分析[J]. 农业生物技术学报, 2019, 27 (6): 1126- 1132.
	WU Y L , LI J L , ZHOU W L , et al. Analysis of the technical points of porcine somatic cell cloning embryo transfer[J]. Journal of Agricultural Biotechnology, 2019, 27 (6): 1126- 1132.
20	马红. 供核细胞来源对猪克隆胚胎发育的影响[J]. 黑龙江农业科学, 2013 (12): 54- 57.
	MA H . Effects of donor cell sources on the development of porcine cloned embryos[J]. Heilongjiang Agricultural Sciences, 2013 (12): 54- 57.
21	王维, 李运生, 曹鸿国, 等. 供体细胞与哺乳动物体细胞核移植[J]. 生命科学, 2010, 22 (9): 837- 845.
	WANG W , LI Y S , CAO H G , et al. Donor cell and mammalian somatic cell nuclear transfer[J]. Chinese Bulletin of Life Science, 2010, 22 (9): 837- 845.
22	SHI J , ZHOU R , LUO L , et al. Influence of embryo handling and transfer method on pig cloning efficiency[J]. Anim Reprod Sci, 2015, 154, 121- 127. doi: 10.1016/j.anireprosci.2015.01.006
23	RIM C H , FU Z , BAO L , et al. The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency[J]. Anim Reprod Sci, 2013, 143 (1-4): 91- 96. doi: 10.1016/j.anireprosci.2013.10.004
24	FENG T , QI X L , ZOU H Y , et al. TSA activates pluripotency factors in porcine recloned embryos[J]. Genes (Basel), 2022, 13 (4): 649. doi: 10.3390/genes13040649
25	SHI J , XIAO L , TAN B , et al. Comparative evaluation of production performances of cloned pigs derived from superior Duroc boars[J]. Anim Reprod Sci, 2022, 244, 107049. doi: 10.1016/j.anireprosci.2022.107049
26	纪慧丽, 卢晟盛, 潘登科. 体细胞核移植后表观遗传重编程的异常及其修复[J]. 遗传, 2014, 36 (12): 1211- 1218.
	JI H L , LU S S , PAN D K . Abnormal epigenetic reprogramming after somatic cell nuclear transfer and its repair[J]. Hereditas, 2014, 36 (12): 1211- 1218.
27	PARK M R , CHO S K , LEE S Y , et al. A rare and often unrecognized cerebromeningitis and hemodynamic disorder: a major cause of sudden death in somatic cell cloned piglets[J]. Proteomics, 2005, 5 (7): 1928- 1939. doi: 10.1002/pmic.200401079
28	JIN J , LI S , GAO Q , et al. Significant improvement of pig cloning efficiency by treatment with LBH589 after somatic cell nuclear transfer[J]. Theriogenology, 2013, 80 (6): 630- 635. doi: 10.1016/j.theriogenology.2013.06.006
29	ZHOU K, WANG T T, ZHANG J J, et al. LEUTX regulates porcine embryonic genome activation in somatic cell nuclear transfer embryos[J]. Cell Rep, 2024 Jun 25;43(6): 114372.
30	QIN G S , ZHAO J G , HUANG J J . RepSox increases porcine cloning efficiency by improving pluripotency of donor nuclei[J]. Cell Reprogram, 2019, 21 (4): 181- 186. doi: 10.1089/cell.2018.0064
31	WEI Y , ZHU J , HUAN Y , et al. Aberrant expression and methylation status of putatively imprinted genes in placenta of cloned piglets[J]. Cell Reprogram, 2010, 12 (2): 213- 222. doi: 10.1089/cell.2009.0090
32	SHEN C J , CHENG W T , WU S C , et al. Differential differences in methylation status of putative imprinted genes among cloned swine genomes[J]. PLoS One, 2012, 7 (2): e32812. doi: 10.1371/journal.pone.0032812
33	逯心玉, 刘彪, 胡魁, 等. 克隆种公猪生长状况和繁殖性能评价[J]. 畜牧与兽医, 2012, 44 (7): 6- 9.
	LU X Y , LIU B , HU K , et al. Evaluation of growth and reproductive performance of cloned boars[J]. Animal Husbandry & Veterinary Medicine, 2012, 44 (7): 6- 9.
34	刘建, 邹辉, 罗志文, 等. 克隆杜洛克种公猪及其扩繁后代的生产性能评价[J]. 猪业科学, 2021, 38 (7): 110- 114.
	LIU J , ZOU H , LUO Z W , et al. Production performance evaluation of cloned Duroc boars and their expanded offspring[J]. Swine Industry Science, 2021, 38 (7): 110- 114.
35	徐文文, 农素群, 马青艳, 等. 体细胞克隆桂科猪及后代生长性能评估[J]. 黑龙江畜牧兽医, 2025 (6): 44- 48.
	XU W W , NONG S Q , MA Q Y , et al. Growth performance evaluation of somatic cell cloned Guike pigs and their offspring[J]. Heilongjiang Animal Husbandry and Veterinary, 2025 (6): 44- 48.
36	LIAO Z , ZHANG J , SUN S , et al. Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer[J]. Nat Commun, 2024, 15 (1): 5. doi: 10.1038/s41467-023-43985-7
37	GAO R , WANG C , GAO Y , et al. Inhibition of aberrant DNA re-methylation improves post-implantation development of somatic cell nuclear transfer embryos[J]. Cell Stem Cell, 2018, 23 (3): 426- 435. doi: 10.1016/j.stem.2018.07.017
38	GLANZNER W G , DE MACEDO M P , GUTIERREZ K , et al. Enhancement of chromatin and epigenetic reprogramming in porcine SCNT embryos-progresses and perspectives[J]. Front Cell Dev Biol, 2022, 10, 940197. doi: 10.3389/fcell.2022.940197
39	CHEN C H , JIANG B H , HUANG S Y , et al. Genetic polymorphisms, growth performance, hematological parameters, serum enzymes, and reproductive characteristics in phenotypically normal Landrace boars produced by somatic cell nuclear transfer[J]. Theriogenology, 2013, 80 (9): 1088- 1096. doi: 10.1016/j.theriogenology.2013.08.010
40	SHI J , TAN B , LUO L , et al. Assessment of the growth and reproductive performance of cloned pietrain boars[J]. Animals, 2020, 10 (11): 2053. doi: 10.3390/ani10112053

序号 Serial number	供体公猪耳号 Donor boar ear number	料肉比 Feed-gain ratio	100 kg体重校正日龄/d 100 kg body weight corrected age	选择凭据 Select credentials
1	XYDKH01032	2.79	111.02	100 kg日龄
2	XYDKH01442	2.08	112.76	100 kg日龄
3	XYDKH01133	2.01	113.03	100 kg日龄
4	XYDKH01425	2.06	113.26	100 kg日龄
5	XYDKH01137	1.97	113.38	100 kg日龄、料肉比
6	XYDKH01029	2.23	114.49	100 kg日龄
7	XYDKH01354	2.15	114.99	100 kg日龄、血缘存栏少
8	XYDKH01131	2.26	115.33	100 kg日龄
9	XYDKH01364	2.19	117.48	100 kg日龄、血缘存栏少
10	XYDKH01355	2.22	119.17	100 kg日龄、血缘存栏少
11	XYDKH01072	1.95	121.46	料肉比
12	XYDKH01427	1.95	124.22	料肉比

胚胎分组 Embryo grouping	培养胚胎数/个 Number of embryos cultured	胚胎分裂数/个 Number of embryo division	分裂率/% Division rate	胚胎体外囊胚数/个 Embryo in vitro blastocyst	发育率/% Developmental rate
第一组Group 1	30	26	86.70	7	23.30
第二组Group 2	26	22	84.60	4	15.40

代孕母猪耳号 Surrogate sow ear number	供体名称及胚胎数目/个 Donor name and number of embryos	移植胚胎总数/个 Total number of embryos transferred	妊娠结果 Pregnancy outcome
FHSCG13901	XYDKHO1029 (85) XYDKHO1425 (97)	182	返情
FHSCG13155	XYDKHO1032 (87) XYDKHO1427 (97)	184	分娩
FHSCG12969	XYDKHO1137 (94) XYDKHO1072 (92)	186	分娩
FHSCG12915	XYDKHO1442 (82) XYDKHO1355 (106)	188	分娩
FHSCG13788	XYDKHO1131 (84) XYDKHO1364 (96)	180	分娩
FHSCG13181	XYDKHO1133 (93) XYDKHO1354 (87)	180	返情

代孕母猪耳号 Surrogate sow ear number	总仔数/头 Total litter size	活仔数/头 Number of live births	健仔数/头 Number of healthy piglets	弱仔数/头 Number of weak piglet	死胎数/头 Number of still birth	活仔率/% Live piglet percent	健仔率/% Healthy rate
FHSCG12915	6	5	4	1	1	83.33	66.67
FHSCG13788	8	7	5	2	1	87.50	62.50
FHSCG13155	6	6	3	3	0	100.00	50.00
FHSCG12969	9	7	3	4	2	77.78	33.33
总计Total	29	25	15	10	4	86.21	51.72

克隆个体 Clone individual	与供体不一致率/% The rate of inconsistency with the donor	与供体不一致位点数 Number of loci inconsistent with the donor	位点总数 Total number of loci
2_1	0.11	64	567 98
2_2	0.11	63	567 95
2_3	0.11	63	568 05
2_4	0.11	64	568 01
1_3	0.12	69	567 91
1_4	0.12	68	567 78
1_6	0.12	69	567 95
1_1	0.13	71	567 90
4_1	0.12	69	567 96
4_4	0.12	70	567 97
4_5	0.12	67	567 88
4_6	0.12	70	568 08

基于体细胞克隆的超级公猪快速扩繁技术的效果评价分析

Analysis on the Effect of Rapid Population Expansion of Excellent Boars Based on Somatic Cell Cloning

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 17

参考文献 40

相关文章 8

编辑推荐

Metrics

本文评价

分组 Grouping	数量/次 Quantity	体积/mL Volume	密度/(亿·mL^-1) Density	活力 Vitality	畸形率/% Teratological rate	有效精子/亿 Effective sperms
克隆猪Clone porcine	70	188.17±49.27^ab	3.52±0.87^a	81.13±2.11^b	8.23±1.77	486.35±159.56^ab
供体同胞后代 Donor compatriots offspring	30	193.87±64.48^ab	2.92±0.89^b	84.20±1.61^a	7.90±1.97	438.60±184.19^ab
供体半同胞 Donor half-siblings	6	208.17±67.86^a	3.34±1.37^ab	86.67±5.16^a	12.00±3.10	494.70±173.45^a
同品系Congenic strains	1 245	168.26±55.93^b	3.10±1.23^b	84.41±3.34^a	8.17±2.18	388.12±166.64^b

分组 Grouping	数量/窝 Quantity	分娩率/% Birth rate	流产率/% Abortion ratio	返情率/% Re-service rate	空怀率/% Empty rate	死淘率/% Death rate
克隆猪Clone porcine	827	81.02	2.78	5.80	8.59	1.81
供体同胞Donor compatriots	297	74.07	2.69	7.07	13.47	2.69
供体同胞后代Donor compatriots offspring	3 456	86.55	1.42	3.36	7.18	1.50

分组 Grouping	数量/窝 Quantity	畸形数/头 Number of deformity	总仔数/头 Total litter size	活仔数/头 Number of live births	健仔数/头 Number of healthy piglets	弱仔数/头 Number of weak births
克隆猪Clone porcine	647	0.03±0.26	12.89±2.87	12.13±2.89^b	11.41±2.57^a	0.70±1.02^a
供体同胞Donor compatriots	220	0.02±0.22	12.81±2.85	11.98±2.75^b	11.15±2.56^b	0.81±1.13^a
供体同胞后代 Donor compatriots offspring	766	0.01±0.10	13.20±2.32	12.30±2.57^a	11.83±2.32^a	0.45±0.97^b

[1]	卯升义, 李卓, 奎华, 角德灵, 李钰莹, 卿玉波, 郭建雄, 韦云芳, 魏红江. 体细胞克隆技术在乌金猪遗传资源保护上的应用[J]. 畜牧兽医学报, 2020, 51(9): 2130-2137.
[2]	李延鹤，刘军，张涌，权富生. 动物胚胎育种及应用中的技术策略[J]. 畜牧兽医学报, 2016, 47(10): 1954-1960.
[3]	尹多;朱玉霞;柳海星;李钟淑;方南洙. 自由基吸收类抗氧化剂对延边黄牛体细胞克隆重构胚发育的影响[J]. 畜牧兽医学报, 2012, 43(8): 1215-1221.
[4]	罗学明;;张卫红;;冯冲;龙川;闫军;赵会静;张兆旺;张子军;潘登科;. 受体母猪排卵状况对体细胞克隆胚胎移植效率的影响[J]. 畜牧兽医学报, 2010, 41(3): 366-370.
[5]	张诺 ;杜卫华 ;郝海生;王栋;武珅;朱化彬. 影响奶牛体细胞手工克隆技术因素的研究[J]. 畜牧兽医学报, 2009, 40(9): 1315-1319.
[6]	李世杰;李冬杰;杜卫华;樊宝良;;戴蕴平;李宁. 成纤维生长因子（FGF2）和成纤维生长因子受体（FGFR1）在新生死亡体细胞克隆牛组织中的表达[J]. 畜牧兽医学报, 2008, 39(10): 1289-1294.
[7]	王亮;彭涛;刘婷婷;汪汉卿;曹旭;朱海;吕自力;张达江;蔡涛;高红. 不同电融合条件对莎能奶山羊转基因（GFP）核移植胚胎发育的影响[J]. 畜牧兽医学报, 2006, 37(12): 1368-1372.
[8]	朱化彬;程金华;孙秀柱;王栋;戴蕴平;龚国春;万荣;郝海生 . 鲁西黄牛体细胞克隆保种技术的研究[J]. 畜牧兽医学报, 2006, 37(12): 1287-1292.