新疆幼畜致泻性大肠埃希菌的毒力基因、耐药性和生物膜的相关性分析

doi:10.11843/j.issn.0366-6964.2025.10.032

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (10): 5125-5136.doi: 10.11843/j.issn.0366-6964.2025.10.032

新疆幼畜致泻性大肠埃希菌的毒力基因、耐药性和生物膜的相关性分析

白怡馨¹(), 张梦菲¹(), 迪娜拉·叶尔波力¹, 王磊¹, 靳婉婧¹, 黄颖¹, 谢金鑫¹, 郑晓风¹, 王美玲¹, 王传锋², 苏战强¹, 张炜³, 佟盼盼¹^,*()

1. 新疆农业大学动物医学学院, 新疆草食动物新药研究与创制重点实验室, 乌鲁木齐 830052
2. 伊犁职业技术学院, 伊宁 835000
3. 南京农业大学动物医学学院, 南京 210095

收稿日期:2024-12-24 出版日期:2025-10-23 发布日期:2025-11-01
通讯作者: 佟盼盼 E-mail:1527026733@qq.com;zmf2049@163.com;tongpanpan123@163.com
作者简介:白怡馨(2002-), 女, 新疆乌鲁木齐人, 主要从事动物病原菌的耐药性研究, E-mail: 1527026733@qq.com
张梦菲(1992-), 男, 河南周口人, 博士, 讲师, 主要从事病原宿主互作、抗病育种相关研究, E-mail: zmf2049@163.com
第一联系人：
白怡馨和张梦菲为同等贡献作者
基金资助:
自治区重大科技专项(2023A02007-2);国家重点研发项目(2021YFD1600702-3);国家自然科学基金(31960695);新疆维吾尔自治区奶产业技术体系(XJARS-11);新疆农业大学校级大学生创新创业训练计划项目(dxscx2024427)

Analysis of Virulence Genes, Drug Resistance and Biofilm of Diarrheagenic Escherichia coli from Young Livestock in Xinjiang

BAI Yixin¹(), ZHANG Mengfei¹(), Dinala·Yeerboli ¹, WANG Lei¹, JIN Wanjing¹, HUANG Ying¹, XIE Jinxin¹, ZHENG Xiaofeng¹, WANG Meiling¹, WANG Chuanfeng², SU Zhanqiang¹, ZHANG Wei³, TONG Panpan¹^,*()

1. College of Veterinary Medicine, Xinjiang Agricultural University/Xinjiang Key Laboratory of New Drug Research and Development for Herbivores, Urumqi 830052, China
2. Yili Vocational and Technical College, Yining, Xinjiang 835000, China
3. College of Animal Medicine, Nanjing Agricultural University, Nanjing 210095

Received:2024-12-24 Online:2025-10-23 Published:2025-11-01
Contact: TONG Panpan E-mail:1527026733@qq.com;zmf2049@163.com;tongpanpan123@163.com

摘要/Abstract

摘要：

近年来细菌耐药性越来越成为威胁畜牧养殖和人类健康的重要问题, 但细菌耐药性的调控机制非常复杂, 本研究旨在分析致泻性大肠埃希菌(diarrheagenic Escherichia coli, DEC)毒力基因、耐药性和生物膜形成能力的相关性。对新疆腹泻幼畜76株E. coli进行毒力基因、系统进化分群、药物敏感性检测、生物膜形成能力测定及溶血试验。结果表明, 76株DEC包括ETEC(n=28)、STEC(n=6)、EPEC(n=5)、ETEC/STEC(n=23)、ETEC/EPEC(n=1)、ETEC/EPEC/STEC(n=13), 以B1(40.8%)和A群(28.9%)为主, 对阿莫西林、氨苄西林、四环素、头孢噻肟的耐药率在53.9%~80.3%, 对链霉素、氟苯尼考、磺胺甲基异恶唑、头孢曲松、头孢他啶、庆大霉素、环丙沙星的耐药率为21.1%~47.4%, 对左氧氟沙星、氨苄西林-舒巴坦、阿米卡星、磷霉素、多黏菌素B的耐药率为1.3%~15.8%, 73.7%强成膜, 17.1%中成膜, 7.9%弱成膜, 1.3%未成膜, 14株呈β溶血。强成膜菌株在携带STb、stx1、hly和eae的DEC中更常见(P < 0.001)。成膜能力与溶血相关(P＜0.05), 与多重耐药性无关(P＞0.05), 与头孢他啶耐药性显著相关(P < 0.001), 与氨苄西林-舒巴坦耐药性相关(P=0.027 9, P＜0.05)。研究结果表明, 新疆地区腹泻幼畜DEC类型复杂, 多数菌株都具有成膜能力, 其成膜能力与毒力基因和溶血性相关, 与头孢他啶和氨苄西林-舒巴坦耐药性有关, 生物膜形成能力可能与抗生素治疗失败和持续感染相关, 本研究为防治幼畜腹泻提供了参考。

关键词: 致泻性大肠杆菌, 生物膜, 耐药性, 毒力基因, 幼畜

Abstract:

In recent years, bacterial drug resistance has become an increasingly significant issue threatening animal husbandry and human health. However, the regulatory mechanisms of bacterial drug resistance are extremely complicated. This study aims to analyze the correlations between virulence genes, drug resistance and biofilm formation ability of diarrheagenic Escherichia coli(DEC). Virulence genes, phylogenetic grouping, drug sensitivity testing, biofilm formation ability assay and hemolysis tests were carried out on 76 strains of E. coli from young livestock with diarrhea in Xinjiang. The results showed that 76 strains of DEC included ETEC (n=28), STEC (n=6), EPEC (n=5), ETEC/STEC (n=23), ETEC/EPEC (n=1), ETEC/EPEC/STEC (n=13), mainly belonging to group B1 (40.8%) and group A (28.9%). The resistance rates to amoxicillin, ampicillin, tetracycline and cefotaxime were 53.9%-80.3%, to streptomycin, florfenicol, sulfamethoxazole, ceftriaxone, ceftazidime, gentamicin and ciprofloxacin were 21.1%-47.4%, and to levofloxacin, ampicillin sulbactam, amikacin, fosfomycin and polymyxin B were 1.3%-15.8%. About 73.7% of the isolates had strong biofilm formation ability, 17.1% had moderate biofilm formation ability, 7.9% had weak biofilm formation ability, and 1.3% had no biofilm formation ability. Fourteen strains showed β-hemolysis. Strong biofilm-forming strains were more common among DEC strains carrying STb, stx1, hly and eae (P＜0.001). The biofilm formation ability was related to hemolysis (P＜0.05), not related to multidrug resistance (P>0.05), significantly related to ceftazidime resistance (P＜0.001), and related to ampicillin-sulbactam (P=0.027 9, P＜0.05). The research results indicated that the types of DEC in young livestock with diarrhea in Xinjiang were complex, and most strains had the biofilm formation ability. The biofilm formation ability was related to virulence genes and hemolysis, and the related to resistance to cefotaxime and ampicillin sulbactam. The biofilm formation ability might be associated with the failure of antibiotic treatment and persistent infection. This study provides a reference for the prevention and treatment of diarrhea in young livestock.

Key words: diarrheagenic Escherichia coli, biofilm, drug resistance, virulence genes, young livestock

中图分类号:

S852.612

白怡馨, 张梦菲, 迪娜拉·叶尔波力, 王磊, 靳婉婧, 黄颖, 谢金鑫, 郑晓风, 王美玲, 王传锋, 苏战强, 张炜, 佟盼盼. 新疆幼畜致泻性大肠埃希菌的毒力基因、耐药性和生物膜的相关性分析[J]. 畜牧兽医学报, 2025, 56(10): 5125-5136.

BAI Yixin, ZHANG Mengfei, Dinala·Yeerboli , WANG Lei, JIN Wanjing, HUANG Ying, XIE Jinxin, ZHENG Xiaofeng, WANG Meiling, WANG Chuanfeng, SU Zhanqiang, ZHANG Wei, TONG Panpan. Analysis of Virulence Genes, Drug Resistance and Biofilm of Diarrheagenic Escherichia coli from Young Livestock in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 5125-5136.

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动物 Animal	DEC类型(数量) DEC type (No.)	进化分群(数量) Phylo-group (No.)	溶血菌株(数量) Hemolytic isolates (No.)	生物膜形成能力(数量) Biofilm formation (No.)
猪 Pig	STEC (1)	B1 (1)	ETEC (8), ETEC/STEC (4), STEC (1) ETEC/STEC/EPEC (1)	强(20) Strong (20)
	ETEC (12)	A (6), B1 (5), C (1)
	ETEC/STEC (6)	A (2), B1 (2), C (2)
	ETEC/STEC/EPEC (1)	E (1)
牛 Cattle	STEC (2)	A (2)	0	强(18), 中(13), 弱(6), 无(1) Strong (18), medium (13), weak (6) and non (1)
	ETEC (9)	A (1), B1 (2), C (2), E (1), unknow (3)
	EPEC (1)	A (1)
	ETEC/STEC (13)	A (5), B1 (5), C (1), E (1), unknow (1)
	ETEC/EPEC (1)	A (1)
	ETEC/STEC/EPEC (12)	A (3), B1 (8), E (1)
羊 Sheep	STEC (3)	A (1), C (1), unknow (1)	0	强(18) Strong (18)
	ETEC (7)	B1 (4), C (2), unknow (1)
	EPEC (4)	B1 (2), C (1), unknow(1)
	ETEC/STEC (4)	B1 (2), C (2)
合计 Total	ETEC (28), ETEC/STEC (23), ETEC/EPEC/STEC (13), STEC (6), EPEC (5), ETEC/EPEC (1),	B1 (31), A (22), C (12), E (4), unknow (7)	ETEC (8), ETEC/STEC (4), STEC (1) ETEC/STEC/EPEC (1)	强(56), 中(13), 弱(6), 无(1) Strong (56), medium (13), weak (6) and non (1)

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新疆幼畜致泻性大肠埃希菌的毒力基因、耐药性和生物膜的相关性分析

Analysis of Virulence Genes, Drug Resistance and Biofilm of Diarrheagenic Escherichia coli from Young Livestock in Xinjiang

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