添加红景天苷的全价粮对犬血液生化指标和肝转录组学的影响

doi:10.11843/j.issn.0366-6964.2025.01.041

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (1): 455-465.doi: 10.11843/j.issn.0366-6964.2025.01.041

添加红景天苷的全价粮对犬血液生化指标和肝转录组学的影响

王盛琪¹^,²(), 季新雨¹^,², 黄福青²^,³^,⁴, 胡曼丽²^,³^,⁴, 王柔淇¹, 耿玉欣¹^,², 孙迎雪¹^,², 齐智利¹^,*(), 张鑫²^,³^,⁴^,*()

1. 华中农业大学动物科学技术学院, 武汉 430070
2. 赣南医科大学赣南创新与转化医学研究院重大疾病新药靶发现及新药创制全国重点实验室, 赣州 341000
3. 赣南医科大学附属第一医院心脑血管疾病防治教育部重点实验室, 赣州 341000
4. 赣州大健康宠物科学研究院, 赣州 341000

收稿日期:2024-01-02 出版日期:2025-01-23 发布日期:2025-01-18
通讯作者: 齐智利,张鑫 E-mail:KKY@webmail.hzau.edu.cn;zhiliqi@mail.hzau.edu.cn;zhangxin@gmu.edu.cn
作者简介:王盛琪(1999-)，女，黑龙江佳木斯人，硕士，主要从事宠物营养研究，E-mail: KKY@webmail.hzau.edu.cn
基金资助:
湖北省自然科学基金(2022CFB327)；华中农业大学-中国农业科学院深圳农业基因组研究所合作基金(SZYJY2022008)；“十四五”国家重点研发计划项目(2023YFD1301000)

Effects of Salidroside-added Complete Nutrition Food on Blood Biochemical Indexes and Liver Transcriptomics in Dogs

WANG Shengqi¹^,²(), JI Xinyu¹^,², HUANG Fuqing²^,³^,⁴, HU Manli²^,³^,⁴, WANG Rouqi¹, GENG Yuxin¹^,², SUN Yingxue¹^,², QI Zhili¹^,*(), ZHANG Xin²^,³^,⁴^,*()

1. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2. State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases, Gannan Innovation and Transformation Medical Research Institute, Gannan Medical University, Ganzhou 341000, China
3. Key Laboratory of Cardiovascular Disease Prevention and Control of Ministry of Education, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China
4. Ganzhou Pet Health Science Research Institute, Ganzhou 341000, China

Received:2024-01-02 Online:2025-01-23 Published:2025-01-18
Contact: QI Zhili, ZHANG Xin E-mail:KKY@webmail.hzau.edu.cn;zhiliqi@mail.hzau.edu.cn;zhangxin@gmu.edu.cn

摘要/Abstract

摘要：

旨在研究全价粮添加红景天苷对犬血液和肝转录组学的影响，以期为犬的肝养护及肝疾病预防工作提供试验数据支持。选取10只成年中华田园犬(平均体重8.67±1.00 kg)，随机分为对照组(n=5)和试验组(n=5)，分别饲喂对照犬粮和红景天苷全价粮。通过血液检查来评估红景天苷全价粮的饲喂效果和安全性，并通过转录组学技术探究红景天苷全价粮对犬潜在的调节机制，试验期为8周。血液检查结果显示，红景天苷全价粮显著降低了犬血清谷丙转氨酶的浓度(P＜0.05)，对其他大部分指标没有显著影响(P>0.05)，但相比于对照犬粮，采食红景天苷全价粮的犬血液指标波动小，能保持血液指标的稳定。转录组学分析结果显示，差异表达基因显著富集在钙信号通路、PI3K-Akt信号通路和MAPK信号通路等。针对表型的基因集富集分析结果表明，红景天苷全价粮在脂代谢、糖代谢、炎症、氧化应激和纤维化方面起调节作用，其中对于糖脂代谢的调节更显著，相关信号通路在试验组显著上调。综上所述，红景天苷全价粮具有降低犬血清谷丙转氨酶浓度的作用，能够稳定犬的血液指标浓度，调节了犬的肝糖脂代谢，这可能主要通过调节钙信号通路、PI3K-Akt信号通路和MAPK信号通路来实现。

关键词: 红景天苷, 转录组学, 糖脂代谢, 犬

Abstract:

The aim of this study was to investigate the effects of salidroside-added complete nutrition food on blood and liver transcriptomics in dogs, with a view to provide experimental data to support liver conservation and prevent liver disease in dogs. Ten adult Chinese field dogs (8.67±1.00 kg) were selected and randomly divided into control (n=5) and test (n=5) groups. The control group was fed with control food, while the test group was fed with complete nutrition food supplemented with salidroside. The study evaluated the feeding effect and safety of complete nutrition food supplemented with salidroside through blood examination. Additionally, the potential regulatory mechanism of salidroside-added complete nutrition food on dogs was explored using transcriptomics technology. The trial period lasted 8 weeks. The blood test results showed that salidroside-added complete nutrition food significantly reduced the concentration of serum alanine aminotransferase in dogs (P < 0.05), and did not significantly affect most of the other indexes (P>0.05). However, compared to the control food, the complete nutrition food with added salidroside did not cause greater fluctuations in blood indexes and could maintain their stability. The transcriptomics analysis results revealed that differentially expressed genes were significantly enriched in the calcium signaling pathway, PI3K-Akt signaling pathway, and MAPK signaling pathway. The results of gene set enrichment analysis for phenotypes revealed that salidroside-added complete nutrition food played a regulatory role in lipid metabolism, glucose metabolism, inflammation, oxidative stress, and fibrosis. It showed a more significant regulation of glucose and lipid metabolism, with the related signaling pathways significantly up-regulated in the test group. In conclusion, salidroside-added complete nutrition food has been found to reduce serum alanine aminotransferase concentration in dogs and help stabilize the concentration of blood indexes in dogs, and regulate hepatic glucolipid metabolism in dogs. These effects may be primarily achieved by regulating the calcium signaling pathway, the PI3K-Akt signaling pathway, and the MAPK signaling pathway.

Key words: salidroside, transcriptomics, glucolipid metabolism, dog

中图分类号:

S858.292

王盛琪, 季新雨, 黄福青, 胡曼丽, 王柔淇, 耿玉欣, 孙迎雪, 齐智利, 张鑫. 添加红景天苷的全价粮对犬血液生化指标和肝转录组学的影响[J]. 畜牧兽医学报, 2025, 56(1): 455-465.

WANG Shengqi, JI Xinyu, HUANG Fuqing, HU Manli, WANG Rouqi, GENG Yuxin, SUN Yingxue, QI Zhili, ZHANG Xin. Effects of Salidroside-added Complete Nutrition Food on Blood Biochemical Indexes and Liver Transcriptomics in Dogs[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 455-465.

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成分 Ingredient	红景天苷全价粮 Salidroside-added complete nutrition food
水分 Moisture	9.23
粗蛋白质 Crude protein	22.33
粗脂肪 Crude fat	10.56
粗纤维 Crude fibre	2.30
粗灰分 Crude ash	4.90
钙 Calcium	0.93
总磷 Total phosphorus	0.55
铜/(mg·kg^-1)Copper	6.37
水溶性氯化物 Water-soluble chloride	0.21
能量/J Gross energy	18 088.67

组别 Group	对照组 Control group	试验组 Test group
体重/kg Body weight	8.76±0.62	8.58±1.36
鼻肛体长/cm Naso-anal length	79.80±4.66	74.20±3.96
腰围/cm Waistline	33.80±2.59	35.80±3.35
臀围/cm Hipline	49.60±5.55	52.80±4.60
背部皮下脂肪厚度/mm Thickness of subcutaneous fat on the back	4.70±0.84	5.00±0.50
BCS评分 BCS score	3.80±0.45	4.20±0.45

指标 Indices	对照组 Control group		试验组 Test group
指标 Indices	第0周	第8周	第0周	第8周
白细胞计数/(10⁹·L^-1) WBC	12.41±1.89	15.94±4.81	10.89±6.34	13.67±2.18
淋巴细胞比例/% LYM	31.28±6.86^a	26.08±6.03	19.33±5.64^b	21.76±7.22
单核细胞比例/% MON	4.33±1.01	3.82±1.22	3.52±2.19	3.88±2.14
中性粒细胞比例/% NEU	59.14±7.41	66.28±6.45	71.35±8.12	70.28±8.44
嗜酸性粒细胞比例/% EOS	3.88±2.34	3.10±2.05	3.10±2.33	2.35±0.50
嗜碱性粒细胞比例/% BAS	0.30±0.19	0.68±0.55	0.84±0.57	0.86±0.38
红细胞计数/(10¹²·L^-1) RBC	9.10±1.54	8.59±0.79	8.55±1.02	9.24±0.37
血小板计数/(10⁹·L^-1) PLT	76.60±55.68^Aa	195.80±69.65^b	242.50±46.68^B	243.00±20.75

指标 Indices	对照组 Control group		试验组 Test group
指标 Indices	第0周	第8周	第0周	第8周
谷丙转氨酶/(U·L^-1)ALT	19.58±7.82	29.10±14.40	34.10±12.36^a	16.96±6.01^b
谷草转氨酶/(U·L^-1)AST	39.34±9.94	32.78±10.62	27.18±6.45	21.30±3.95
碱性磷酸酶/(U·L^-1)ALP	65.44±47.99	39.08±17.14	50.83±13.76	53.52±37.40
血清总蛋白/(g·L^-1)TP	59.62±3.64	55.59±2.44	59.89±5.73	59.25±6.18
白蛋白/(g·L^-1)ALB	37.38±3.85	34.83±2.16	34.60±4.82	35.74±1.61
肌酐/(μmol·L^-1)CREA	64.30±8.43	59.53±0.66	56.07±9.52	67.19±2.07
尿/(mmol·L^-1)UREA	3.61±0.94	4.44±0.77	4.21±1.02	3.78±1.15
总胆固醇/(mmol·L^-1)TC	4.79±0.34	4.19±0.72	3.96±0.72	5.08±1.99
甘油三酯/(mmol·L^-1)TG	0.72±0.02^a	0.52±0.11^b	0.78±0.36	0.75±0.33
肌酸激酶/(U·L^-1)CK	320.60±111.22^a	187.44±55.27^b	155.53±25.08^b	150.20±21.19
α-淀粉酶/(U·L^-1)AMY	852.18±204.53	1124.66±253.84^a	844.62±173.12	768.32±159.89^b

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添加红景天苷的全价粮对犬血液生化指标和肝转录组学的影响

Effects of Salidroside-added Complete Nutrition Food on Blood Biochemical Indexes and Liver Transcriptomics in Dogs

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