沉默PREX1表达对CHMp细胞增殖及侵袭性的影响

doi:10.11843/j.issn.0366-6964.2024.08.042

摘要/Abstract

摘要：

犬乳腺肿瘤是犬常发肿瘤之一，且有50%左右为恶性，其转移倾向明显，易引起动物死亡。Rho-GTPase蛋白家族中的PREX1蛋白，过度激活会导致Rac-GEF功能紊乱，影响细胞周期，改变细胞生理功能，PREX1的改变可能对细胞癌变有促进作用。为探讨PREX1对犬乳腺肿瘤细胞生理功能的影响，本研究使用shRNA沉默PREX1表达，利用Western blot试验检测沉默后细胞PREX1蛋白表达量的变化，通过CCK-8试验、克隆形成试验探究其对犬乳腺肿瘤细胞系CHMp增殖能力的影响，通过划痕试验和Transwell试验探索沉默其对犬乳腺肿瘤细胞系CHMp侵袭性的影响。研究结果表明，PREX1的低表达，可以抑制CHMp的增殖及侵袭能力。

关键词: 犬乳腺肿瘤, CHMp细胞, 增殖, 迁移, 侵袭

Abstract:

Canine mammary gland tumors are a prevalent neoplasm in dogs, with around 50% of cases exhibiting malignancy. They have a conspicuous inclination to spread to other parts of the body and have the potential to readily result in the demise of animals. When the PREX1 protein in the Rho-GTPase family is overstimulated, it can make Rac-GEF less effective, which can mess up the cell cycle and change how cells work. Alterations in PREX1 can potentially facilitate the development of cancerous cells. This study aimed to investigate the influence of PREX1 on the physiological functions of canine mammary tumor cells. In this study, shRNA was used to silence the expression of PREX1. A Western blot test was then used to see how the expression of PREX1 protein changed in the cells after the suppression. The CCK-8 test and colony formation test were used to see how the substance affected the ability of the CHMp canine mammary carcinoma cell line to divide. Additionally, the scratch test and Transwell test were utilized to examine the influence of silencing the substance on the invasiveness of the cell line. Research indicates that reduced expression of PREX1 can impede the proliferation and invasion capacity of CHMp cell lines.

Key words: canine mammary tumor, CHMp, proliferation, migration, invasion

中图分类号:

徐禧莹, 王毅恒, 区倩婷, 洪琳媛, 刘栩靖, 卢羡盈, 贾坤. 沉默PREX1表达对CHMp细胞增殖及侵袭性的影响[J]. 畜牧兽医学报, 2024, 55(8): 3706-3713.

Xiying XU, Yiheng WANG, Qianting OU, Linyuan HONG, Xujing LIU, Xianying LU, Kun JIA. Effects of Silencing PREX1 Expression on Proliferation and Invasiveness of CHMp[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3706-3713.

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