野生松鼠源屎肠球菌的致病性与耐药性分析

doi:10.11843/j.issn.0366-6964.2023.07.032

摘要/Abstract

摘要： 屎肠球菌作为人及动物肠道中的机会致病菌，可在一定条件下引起宿主心内膜炎、败血症等。目前随着动物源致病屎肠球菌相关报道越来越多，其耐药性和致病性研究也日益增多，但野生动物源的相关研究仍较少。本试验为探究北京大兴野生动物园一只赤腹松鼠突然死亡的原因，对死亡赤腹松鼠进行大体剖检、病理组织切片观察、病原菌分离及其耐药性和致病性分析。结果显示，在肺和肾组织切片中观察到类圆或椭圆形呈短链状的蓝色菌丛；从肺、肝、脾以及腹腔积液中均分离出屎肠球菌；药物敏感性试验显示分离菌株均对磺胺异恶唑及泰妙菌素高度耐药；全基因组测序数据分析显示屎肠球菌均属于ST-324型，且都携带毒力基因efaAfm和耐药基因tetM、msrC，核心基因组单核苷酸多态性（SNPs）分析差异较小；小鼠致病性试验发现，分离菌株具有一定的致病性。本研究综合以上结果判断赤腹松鼠死亡原因为屎肠球菌感染导致的败血症。

关键词: 野生动物, 赤腹松鼠, 屎肠球菌, 全基因组测序

Abstract: Enterococcus faecium, as an opportunistic pathogen in the human and animal intestines, could cause endocarditis and sepsis in the host under certain conditions. Recently, multiple studies indicated that Enterococcus faecium, antimicrobial resistance and pathogenicity of Enterococcus faecium in food animal and wild animal are increasing, but there are fewer studies about AMR in the wild animal. In order to explore the cause of the sudden death of a red-bellied squirrel in Beijing Daxing Wildlife Park, gross autopsy, pathological tissue section, pathogenic bacteria isolation, antibiotic susceptibility testing and pathogenicity analysis were performed on the dead red-bellied squirrel. Results showed that blue flora with short chains of circular or oval shape was observed in lung and kidney sections, and bacteria pathogen were isolated from lung, liver, spleen and abdominal effusion. Antibiotic susceptibility tests have shown that the isolated strains are highly resistant to sulfisoxazole and telomectin; The results of whole genome sequencing showed that the isolated strains all belonged to Enterococcus faecium ST-324, and all carried the virulence gene efaAfm and antibiotic resistance genes tetM and msrC; the core genome single nucleotide polymorphisms (SNPs) analysis showed no significant differences. Animal pathogenicity tests suggested that isolated strains have pathogenicity. The results suggested that the cause of death of the squirrel was sepsis caused by Enterococcus faecium infection.

Key words: wild animals, red-bellied squirrel, Enterococcus faecium, whole genome sequencing

中图分类号:

S852.611

胡秀花, 孙芷馨, 赵梦洋, 谢佳琪, 王敏, 陈海良, 葛昕, 刘天龙, 王少林. 野生松鼠源屎肠球菌的致病性与耐药性分析[J]. 畜牧兽医学报, 2023, 54(7): 3012-3021.

HU Xiuhua, SUN Zhixin, ZHAO Mengyang, XIE Jiaqi, WANG Min, CHEN Hailiang, GE Xin, LIU Tianlong, WANG Shaolin. Pathogenicity and Resistance Analysis of Enterococcus faecium from Wild Squirrels[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3012-3021.

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