ART3抑制剂3-MBA对小鼠睾丸生精功能的影响

doi:10.11843/j.issn.0366-6964.2022.10.019

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (10): 3470-3479.doi: 10.11843/j.issn.0366-6964.2022.10.019

ART3抑制剂3-MBA对小鼠睾丸生精功能的影响

段晨莹^1,2, 李欣², 赵羚均^1,2, 许师源^1,2, 原开敏^1,2, 董智豪^2,3, 郭冠华^2,4, 王栋^2*

1. 吉林农业大学动物科学技术学院, 长春 130118;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 河南科技大学动物科技学院, 洛阳 471203;
4. 山西农业大学动物科学学院, 太谷 030801

收稿日期:2022-03-07 出版日期:2022-10-23 发布日期:2022-10-26
通讯作者: 王栋,主要从事动物遗传育种与繁殖研究,E-mail:dwangcn2002@vip.sina.com
作者简介:段晨莹(1997-),女,山西临汾人,硕士,主要从事动物遗传育种与繁殖研究,E-mail:duan19970103@163.com
基金资助:
国家自然科学基金（31772595）

Effect of ART3 Inhibitor 3-MBA on Spermatogenic Function of Mouse Testis

DUAN Chenying^1,2, LI Xin², ZHAO Lingjun^1,2, XU Shiyuan^1,2, YUAN Kaimin^1,2, DONG Zhihao^2,3, GUO Guanhua^2,4, WANG Dong^2*

1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471203, China;
4. College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Received:2022-03-07 Online:2022-10-23 Published:2022-10-26

摘要/Abstract

摘要： 旨在探究ADP-核糖基转移酶3（ADP-ribosyl transferase 3，ART3）调控精子发生机制，为改善精液品质，提高家畜繁殖性能提供理论依据。本试验设计了3个ART3抑制剂3-甲氧基苯甲酰胺（3-methoxybenzamide，3-MBA）浓度梯度（0.302、0.906和1.510 mg·mL^-1），分别对6～8周龄小鼠进行睾丸注射，并于注射后3 h、6 h、12 h、1 d、2 d、3 d和5 d采集小鼠睾丸和附睾组织，将睾丸组织制成石蜡切片进行HE染色，并对附睾尾精子进行动力学和形态学分析。结果发现，0.302 mg·mL^-13-MBA浓度组于注射后3 d小鼠睾丸曲细精管内空泡面积达到最大化，生精细胞减少，且排布散乱不规则，注射后5 d空泡面积减小，生精细胞有恢复趋势，而0.906和1.510 mg·mL^-1浓度组于注射后5 d空泡面积最大化，曲细精管出现空管现象；3个剂量组小鼠附睾尾精子密度、活力及前向运动精子均呈时间依赖性降低，畸形精子随时间推移逐渐增加，且精子尾部出现断裂、弯折和卷曲等异常形态。综上表明，ART3可能参与调控生精细胞增殖、分化和迁移及精子尾部形成等精子发生过程。

关键词: ART3, 精子发生, 繁殖性能, 3-MBA

Abstract: This study aimed to explore the mechanism of ADP-ribosyl transferase 3 (ART3) regulating spermatogenesis, and to provide a theoretical basis for improving semen quality and livestock reproductive performance. In this experiment, three kinds of concentrations of ART3 inhibitor 3-methoxybenzamide (3-MBA) (0.302, 0.906 and 1.510 mg·mL^-1) were injected into testis of mice in 3 groups aged 6-8 weeks respectively, the testis and epididymis tissues of each mouse were collected at 3 h, 6 h, 12 h, 1 d, 2 d, 3 d and 5 d after injection. The testis tissues were made into paraffin section for HE staining, and the dynamics and morphology of sperm in epididymis caudal were analyzed. The results showed that the area of vacuoles in the seminiferous tubules of mouse testis was maximized, the spermatogenic cells decreased, and arranged dispersedly and irregularly on 3 d after injection in the treatment of 0.302 mg·mL^-1 3-MBA; The area of vacuoles decreased, the spermatogenic cells tended to recover on 5 d after injection; While the area of vacuoles was maximized at 5 d after injection in the treatments of 0.906 and 1.510 mg·mL^-1 3-MBA, and the empty seminiferous tubules also appeared. In the 3 concentration groups, the density, motility and progressive of sperms in epididymis caudal was decreased in a time-dependent manner, and the sperms with abnormal morphology in epididymis caudal also gradually increased, and the abnormal morphology of sperm tails such as breakage, bending and curling appeared. In summary, ART3 may be involved in the regulation of spermatogenesis, such as proliferation, differentiation, migration of spermatogenic cells and formation of spermatogenic tail.

Key words: ART3, spermatogenesis, reproductive performance, 3-MBA

中图分类号:

S814.1

段晨莹, 李欣, 赵羚均, 许师源, 原开敏, 董智豪, 郭冠华, 王栋. ART3抑制剂3-MBA对小鼠睾丸生精功能的影响[J]. 畜牧兽医学报, 2022, 53(10): 3470-3479.

DUAN Chenying, LI Xin, ZHAO Lingjun, XU Shiyuan, YUAN Kaimin, DONG Zhihao, GUO Guanhua, WANG Dong. Effect of ART3 Inhibitor 3-MBA on Spermatogenic Function of Mouse Testis[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3470-3479.

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ART3抑制剂3-MBA对小鼠睾丸生精功能的影响

Effect of ART3 Inhibitor 3-MBA on Spermatogenic Function of Mouse Testis

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