奶牛分娩前后瘤胃代谢物变化规律及其代谢通路研究

doi:10.11843/j.issn.0366-6964.2021.011.015

摘要/Abstract

摘要： 本试验旨在阐明奶牛分娩前后瘤胃代谢物及其代谢通路的变化规律。选取10头体况和胎次相近的健康待产荷斯坦奶牛，分别于分娩前后晨饲前采集瘤胃液，采用UPLC-MS/MS代谢组学技术和MetaboAnanlyst 5.0中的通路分析方法研究奶牛分娩前后瘤胃发酵产物及其代谢通路的变化规律。结果表明，奶牛分娩后瘤胃中57种代谢物含量较分娩前发生了明显变化，其中34种代谢物含量显著降低，23种代谢物含量显著升高，9个代谢通路（牛磺酸和亚牛磺酸代谢、精氨酸和脯氨酸代谢、精氨酸的生物合成、D-谷氨酰胺和D-谷氨酸代谢、淀粉和蔗糖代谢、半乳糖代谢、丙氨酸、天冬氨酸和谷氨酸代谢、嘌呤代谢和维生素B₆代谢）发生了显著变化。本研究表明，奶牛在分娩前7~10 d与氨基酸、糖、核苷酸和维生素相关的瘤胃微生物菌群组成发生了应答性改变。

关键词: 奶牛分娩, 瘤胃代谢物, 代谢组学, 通路分析, 瘤胃微生物

Abstract: The aim of this experiment was to clarify the changes of rumen metabolites and their metabolic pathways of dairy cows before and after parturition. Ten healthy Holstein cows with similar body condition and parity were selected to collect fasting rumen fluid before and after parturition. The changes of rumen fermentation products and their metabolic pathways were analyzed by using UPLC-MS/MS metabonomics technology and pathway analysis method with MetaboAnanlyst 5.0. The results showed that the concentration of 57 rumen metabolites in dairy cows changed significantly before and after delivery, of which 34 metabolites were down-regulated and 23 were up-regulated after delivery. And 9 metabolic pathways (taurine and hypotaurine metabolism, arginine and proline metabolism, arginine biosynthesis, D-glutamine and D-glutamate metabolism, alanine, aspartic acid and glutamate metabolism, starch and sucrose metabolism, galactose metabolism, purine metabolism and vitamin B₆ metabolism) consequently underwent strikingly variation. These results indicated that the composition of rumen microflora related to amino acids, sugars, nucleotides and vitamins occured responsive change in dairy cows at 7~10 days before delivery.

Key words: dairy cows parturition, rumen metabolites, metabolomics, pathway analysis, rumen microorganisms

中图分类号:

S823.91
Q936

张瑞雪, 刘欣, 徐晓锋, 张博, 唐玉林, 任曼, 郭延生. 奶牛分娩前后瘤胃代谢物变化规律及其代谢通路研究[J]. 畜牧兽医学报, 2021, 52(11): 3137-3148.

ZHANG Ruixue, LIU Xin, XU Xiaofeng, ZHANG Bo, TANG Yulin, REN Man, GUO Yansheng. Study on the Changes of Rumen Metabolites and Metabolic Pathways in Dairy Cows before and after Parturition[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3137-3148.

参考文献 40

[1]	MAUVAIS-JARVIS F, CLEGG D J, HEVENER A L.The role of estrogens in control of energy balance and glucose homeostasis[J].Endocr Rev, 2013, 34(3):309-338.
[2]	SCHÄFERS S, VON SOOSTEN D, MEYER U, et al. Influence of conjugated linoleic acids and vitamin E on biochemical, hematological, and immunological variables of dairy cows during the transition period[J].J Dairy Sci, 2018, 101(2):1585-1600.
[3]	ABUELO A, WISNIESKI L, BROWN J L, et al. Rumination time around dry-off relative to the development of diseases in early-lactation cows[J].J Dairy Sci, 2021, 104(5):5909-5920.
[4]	ZHANG G S, ZWIERZCHOWSKI G, MANDAL R, et al. Serum metabolomics identifies metabolite panels that differentiate lame dairy cows from healthy ones[J].Metabolomics, 2020, 16(6):73.
[5]	KENÉZ Á, DÄNICKE S, ROLLE-KAMPCZYK U, et al. A metabolomics approach to characterize phenotypes of metabolic transition from late pregnancy to early lactation in dairy cows[J].Metabolomics, 2016, 12(11):165.
[6]	LUO Z Z, SHEN L H, JIANG J, et al. Plasma metabolite changes in dairy cows during parturition identified using untargeted metabolomics[J].J Dairy Sci, 2019, 102(5):4639-4650.
[7]	CHEN W, GONG L, GUO Z L, et al. A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites:application in the study of rice metabolomics[J].Mol Plant, 2013, 6(6):1769-1780.
[8]	FRAGA C G, CLOWERS B H, MOORE R J, et al. Signature-discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry, XCMS, and chemometrics[J].Anal Chem, 2010, 82(10):4165-4173.
[9]	牛化欣, 常杰, 胡宗福, 等. 基于组学技术研究反刍动物瘤胃微生物及其代谢功能的进展[J].畜牧兽医学报, 2019, 50(6):1113-1122.NIU H X, CHANG J, HU Z F, et al. Research progress on rumen microbiomes and their metabolic functions in ruminants based on omics technology[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(6):1113-1122.(in Chinese)
[10]	马涛, 张乃锋, 屠焰, 等. 羔羊瘤胃微生物区系建立、发展与调控技术研究进展[J].动物营养学报, 2020, 32(10):4733-4742.MA T, ZHANG N F, TU Y, et al. Research advance in establishment, development and regulation technology of rumen microbiota in lambs[J].Chinese Journal of Animal Nutrition, 2020, 32(10):4733-4742.(in Chinese)
[11]	LIU C, WU H, LIU S J, et al. Dynamic alterations in yak rumen bacteria community and metabolome characteristics in response to feed type[J].Front Microbiol, 2019, 10:1116.
[12]	徐晓锋, 胡丹丹, 郭婷婷, 等. 不同精粗比饲粮条件下奶牛瘤胃细菌菌群结构变化的研究[J].动物营养学报, 2019, 31(12):5541-5550.XU X F, HU D D, GUO T T, et al. Structure changes of rumen bacterial flora of dairy cows under different concentrate to roughage ratio diets[J].Chinese Journal of Animal Nutrition, 2019, 31(12):5541-5550.(in Chinese)
[13]	SCHIRMANN K, CHAPINAL N, WEARY D M, et al. Short communication:rumination and feeding behavior before and after calving in dairy cows[J].J Dairy Sci, 2013, 96(11):7088-7092.
[14]	DIREKVANDI E, MOHAMMADABADI T, SALEM A Z M.Effect of microbial feed additives on growth performance, microbial protein synthesis, and rumen microbial population in growing lambs[J].Transl Anim Sci, 2020, 4(4):txaa203.
[15]	ZEBELI Q, GHAREEB K, HUMER E, et al. Nutrition, rumen health and inflammation in the transition period and their role on overall health and fertility in dairy cows[J].Res Vet Sci, 2015, 103:126-136.
[16]	富丽霞, 马涛, 刁其玉, 等. 精料类型对肉羊饲粮养分消化率及尿嘌呤衍生物排出量的影响[J].畜牧兽医学报, 2018, 49(7):1394-1404.FU L X, MA T, DIAO Q Y, et al. Effect of different concentrates on nutrient digestibility and excretion of purine derivatives in urine of mutton sheep[J].Acta Veterinaria et Zootechnica Sinica, 2018, 49(7):1394-1404.(in Chinese)
[17]	钟书.温湿度指数(THI)介导山羊瘤胃细菌群落的变化[D].杨凌:西北农林科技大学, 2019.ZHONG S.Temperature and humidity index (THI) induced rumen bacterial community changes in goats[D].Yangling:Northwest A&F University, 2019.(in Chinese)
[18]	李子健, 李大彪, 高民, 等. 不同生理阶段荷斯坦奶牛瘤胃细菌多样性研究[J].动物营养学报, 2018, 30(8):3017-3025.LI Z J, LI D B, GAO M, et al. Rumen bacteria diversity in holstein dairy cows at different physiological phases[J].Chinese Journal of Animal Nutrition, 2018, 30(8):3017-3025.(in Chinese)
[19]	WEN C Y, LI F N, ZHANG L Y, et al. Taurine is involved in energy metabolism in muscles, adipose tissue, and the liver[J].Mol Nutr Food Res, 2019, 63(2):1800536.
[20]	韩正强.牛磺酸的生产及其在畜禽养殖中的应用[J].中国畜牧兽医, 2012, 39(3):110-113.HAN Z Q. The production and applications of taurine in livestock and poultry aquaculture[J].China Animal Husbandry & Veterinary Medicine, 2012, 39(3):110-113.(in Chinese)
[21]	JOO Y C, KO Y J, YOU S K, et al. Creating a new pathway in Corynebacterium glutamicum for the production of taurine as a food additive[J].J Agric Food Chem, 2018, 66(51):13454-13463.
[22]	江南大学.一种产牛磺酸菌株的筛选方法及用该菌株发酵法生产牛磺酸:中国, CN102965317A[P].2013-03-13.Jiangnan University.Method for screening taurine strains and method for producing taurine through fermenting strains:CN, CN102965317A[P].2013-03-13.(in Chinese)
[23]	AERTS L, VAN ASSCHE F A.Taurine and taurine-deficiency in the perinatal period[J].J Perinat Med, 2002;30(4):281-286.
[24]	程三宝, 朱俊玲, 马俪珍.微生物与发酵食品中的生物胺[J].食品工程, 2007(1):11-13.CHENG S B, ZHU J L, MA L Z.Microflora and biogenic amines of fermented foods[J].Food Engineering, 2007(1):11-13.(in Chinese)
[25]	BELANCHE A, DOREAU M, EDWARDS J E, et al. Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation[J].J Nutr, 2012, 142(9):1684-1692.
[26]	PAENGKOUM P, CHEN S, PAENGKOUM S.Effects of crude protein and undegradable intake protein on growth performance, nutrient utilization, and rumen fermentation in growing Thai-indigenous beef cattle[J].Trop Anim Health Prod, 2019, 51(5):1151-1159.
[27]	SECK M, LINTON J A V, ALLEN M S, et al. Apparent ruminal synthesis of B vitamins in lactating dairy cows fed diets with different forage-to-concentrate ratios[J].J Dairy Sci, 2017, 100(3):1914-1922.
[28]	TIFFANY M E, FELLNER V, SPEARS J W.Influence of cobalt concentration on vitamin B12 production and fermentation of mixed ruminal microorganisms grown in continuous culture flow-through fermentors[J].J Anim Sci, 2006, 84(3):635-640.
[29]	CASTAGNINO D S, SECK M, BEAUDET V, et al. Effects of forage family on apparent ruminal synthesis of B vitamins in lactating dairy cows[J].J Dairy Sci, 2016, 99(3):1884-1894.
[30]	王超, 王宝维, 葛文华, 等. 维生素B6对1~4周龄五龙鹅生长性能、屠宰性能及蛋白质代谢的影响[J].动物营养学报, 2014, 26(7):1814-1821.WANG C, WANG B W, GE W H, et al. Effects of vitamin B6 on growth performance, slaughter performance and protein metabolism of Wulong geese aged from 1 to 4 weeks[J].Chinese Journal of Animal Nutrition, 2014, 26(7):1814-1821.(in Chinese)
[31]	TANAKA T, TATENO Y, GOJOBORI T.Evolution of vitamin B6 (pyridoxine) metabolism by gain and loss of genes[J].Mol Biol Evol, 2005, 22(2):243-250.
[32]	BIRD R P.Chapter four-the emerging role of vitamin B6 in inflammation and carcinogenesis[J].Adv Food Nutr Res, 2018, 83:151-194.
[33]	UELAND P M, MCCANN A, MIDTTUN Ø, et al. Inflammation, vitamin B6 and related pathways[J].Mol Aspects Med, 2017, 53:10-27.
[34]	GUO W, LI Y, WANG L Z, et al. Evaluation of composition and individual variability of rumen microbiota in yaks by 16S rRNA high-throughput sequencing technology[J].Anaerobe, 2015, 34:74-79.
[35]	冯仰廉.反刍动物营养学[M].北京:科学出版社, 2004.FENG Y L.Ruminants nutrition[M].Beijing:Science Press, 2004.(in Chinese)
[36]	SHEN J, ZHENG L X, CHEN X D, et al. Metagenomic analyses of microbial and carbohydrate-active enzymes in the rumen of dairy goats fed different rumen degradable starch[J].Front Microbiol, 2020(11):1003.
[37]	KHAN M A, LEE H J, LEE W S, et al. Starch source evaluation in calf starter:Ⅱ.Ruminal parameters, rumen development, nutrient digestibilities, and nitrogen utilization in Holstein calves[J].J Dairy Sci, 2008, 91(3):1140-1149.
[38]	LOERCH S C, FLUHARTY F L.Physiological changes and digestive capabilities of newly received feedlot cattle[J].J Anim Sci, 1999, 77(5):1113-1119.
[39]	DUFFY E M, TIETZE S M, KNOELL A L, et al. Rumen bacterial composition in lambs is affected by β-adrenergic aganist supplementation and heat stress at the phylum level[J].Transl Anim Sci, 2018, 2(S1):S145-S148.
[40]	ASCHENBACH J R, BORAU T, BUTTER H, et al. Adrenoceptor heterogeneity in the ruminal epithelium of sheep[J].J Comp Physiol B, 2005, 175(4):249-255.