1株类NADC30与类JXA1重组的猪繁殖与呼吸综合征病毒基因组遗传特征分析

doi:10.11843/j.issn.0366-6964.2020.07.020

摘要/Abstract

摘要： 旨在分析本实验室2017年从临床病料中分离的1株猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）的分子遗传特征，测定了其全基因组序列，并运用生物信息学软件对其遗传特点进行了分析。结果显示，该毒株基因组全长15 054 bp，与代表性毒株VR-2332、NADC30、CH-1a、JXA1和QYYZ的核苷酸相似性分别为84.5%、93.4%、84.2%、83.9%和82.1%。系统进化树分析显示，该毒株与NADC30-like毒株处于同一进化分支，属于lineage 1。基于GP5和Nsp2蛋白的氨基酸序列分析显示，分离株GP5蛋白糖基化位点位于34位、44位和51位；在37-45 aa的线性表位区较为保守，在27-30 aa和37-45 aa的线性表位区存在特异的氨基酸位点变异；Nsp2蛋白存在NADC30-lke毒株特征性的131个（323-433位、483位、504-522位）不连续氨基酸的缺失。基因重组分析显示，该毒株以类NADC30为主要亲本、以类JXA1为次要亲本，在Nsp2的氨基端（1 340-2 082 nt）发生了基因重组。本研究成功绘制了1株NADC30-like毒株的基因组遗传特征，确定了其在Nsp2区域发生了基因重组，提示PRRSV通过基因重组在不断发生遗传演化，开展持续的PRRSV流行及遗传变异监测对于该病的防控具有重要意义。

关键词: 猪繁殖与呼吸综合征病毒, 同源性分析, 系统进化树, 遗传变异, 基因重组

Abstract: To investigate the genetic characteristics of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated in Henan Province in 2017, the complete genome was sequenced and further analyzed by bioinformatics. The results showed that the length of the genome is 15 054 bp, and the whole genome shared 84.5%, 93.4%, 84.2%, 83.9%, and 82.1% nucleotide similarities with VR-2332, NADC30, CH-1a, JXA1, and QYYZ, respectively. Phylogenetic analysis displayed that the isolate was clustered into the same branch with NADC30-like strains (lineage 1). For GP5 protein of the isolated strain, the N glycosylation sites were identified at 34, 44, and 51 sites. The linear epitope (37-45 aa) was conserved during NADC30-like strains while the other two linear epitopes (27-30 aa, 37-45 aa) have specific amino acid mutations. The Nsp2 of the isolate has 131 discontinuous amino acid deletion(323-433,483,504-522)which was a genetic marker of NADC30-like strains. Furthermore, the results of recombination analysis showed that the isolate was a mosaic virus between the major parent strain NADC30-like and minor parent strain JXA1-like. The recombinant region was in Nsp2 (1 340-2 082 nt). Our studies described the genetic characteristics of a NADC30-like strain and confirmed the recombinant events that happened in the Nsp2 region. It suggests that PRRSV undergoes genetic evolution through recombination and it is important to continuous monitoring of PRRSV prevalence and genetic variation.

Key words: porcine reproductive and respiratory syndrome virus, homological analysis, phylogenetic tree, genetic variation, gene recombination

中图分类号:

郭振华, 阮海宇, 耿瑞, 陈鑫鑫, 乔松林, 邓瑞广, 张改平. 1株类NADC30与类JXA1重组的猪繁殖与呼吸综合征病毒基因组遗传特征分析[J]. 畜牧兽医学报, 2020, 51(7): 1677-1687.

GUO Zhenhua, RUAN Haiyu, GENG Rui, CHEN Xinxin, QIAO Songlin, DENG Ruiguang, ZHANG Gaiping. Genetic Characteristics of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus between NADC30-like and JXA1-like[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1677-1687.

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