捻转血矛线虫阿苯达唑敏感株和耐药株比较转录组学分析

doi:10.11843/j.issn.0366-6964.2019.09.024

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (9): 1940-1944.doi: 10.11843/j.issn.0366-6964.2019.09.024

捻转血矛线虫阿苯达唑敏感株和耐药株比较转录组学分析

赵学亮¹, 王姝懿^1,2, 孙柯¹, 苏倩¹, 王文龙^1*, 刘春霞^3*

1. 内蒙古农业大学兽医学院, 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018;
2. 内蒙古自治区综合疾病预防控制中心, 呼和浩特 010018;
3. 内蒙古农业大学生命科学学院, 呼和浩特 010018

收稿日期:2019-03-08 出版日期:2019-09-23 发布日期:2019-09-23
通讯作者: 王文龙,主要从事寄生虫组学与分子免疫学研究,E-mail:wwl.imau@163.com;刘春霞,主要从事动物细胞发育分化调控机制研究,E-mail:lcx.imau@163.com
作者简介:赵学亮(1992-),男,河北沧州人,硕士生,主要从事寄生虫组学与分子免疫学研究,E-mail:zhaoxueliang92@sina.com
基金资助:
国家自然科学基金（31760731）；内蒙古自治区科技计划项目（201702074）

Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq

ZHAO Xueliang¹, WANG Shuyi^1,2, SUN Ke¹, SU Qian¹, WANG Wenlong^1*, LIU Chunxia^3*

1. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Center for Disease Control and Prevention, Hohhot 010018, China;
3. College of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2019-03-08 Online:2019-09-23 Published:2019-09-23

摘要/Abstract

摘要： 为探明捻转血矛线虫阿苯达唑敏感株和耐药株在转录组水平的差异，挖掘耐药相关基因，本研究采用Illumina HiSeq 4000对敏感虫株和耐药虫株进行转录组测序，构建cDNA文库，通过质控后，对其进行生物信息学分析。结果显示：敏感和耐药虫株相比较共获得218个显著（P ≤ 0.05）差异表达基因，对显著差异表达基因进行GO功能富集，有121、82和102个基因分别注释到生物学过程、细胞组分和分子功能三大类的206个GO terms上。KEGG数据库分析显示，有42个显著差异表达基因参与31条KEGG信号通路，显著富集在药物代谢-细胞色素P450、药物代谢-其他酶等通路。在敏感株和耐药株中，筛选出GST、CYPs和UGT等9个耐药相关基因，这些基因在捻转血矛线虫耐药中发挥重要的作用。本研究利用RNA-Seq预测出敏感虫株和耐药虫株差异表达基因在功能分类和代谢通路等方面的生物学特征，挖掘出耐药相关基因，进一步为捻转血矛线虫药物靶点预测及耐药性早期鉴别诊断的建立提供重要基础。

Abstract: To explore the differences in transcriptome levels between Haemonchus contortus albendazole sensitive and resistant strains, and to find out drug resistance-related genes. Samples of susceptible and resistant strains were analyzed using Illumina HiSeq 4000 platform and their transcriptome libraries were constructed. After quality filtering, bioinformatics analysis was carried out. The results showed that:A total of 218 significantly different expressed genes (DEGs) between albendazole-susceptible and resistant strains were obtained. Among these 218 genes, 121,82 and 102 DEGs have been annotated to 206 GO terms of three categories respectively, namely biological process, cellular component and molecular function. KEGG analysis showed that 42 significantly DEGs were involved in 31 KEGG pathways, and clustered significantly in pathways of drug metabolism-cytochrome P450, drug metabolism-other enzymes and so on. In addition, we identified 9 drug resistance genes in susceptible and resistant strains, such as GST, CYPs, UGT and so on, which play an important role in the drug resistance of Haemonchus contortus. In this study, the biological characteristics of DEGs in susceptible and resistant strains were predicted by RNA-Seq, which involved functional classification and metabolic pathways. This study provides a foundation for drug target prediction and the establishment of early differential diagnosis methods for drug resistance provides an important basis.

中图分类号:

S855.9

赵学亮, 王姝懿, 孙柯, 苏倩, 王文龙, 刘春霞. 捻转血矛线虫阿苯达唑敏感株和耐药株比较转录组学分析[J]. 畜牧兽医学报, 2019, 50(9): 1940-1944.

ZHAO Xueliang, WANG Shuyi, SUN Ke, SU Qian, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1940-1944.

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捻转血矛线虫阿苯达唑敏感株和耐药株比较转录组学分析

Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq

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